5 U/µl (1µl contains 5 units Taq DNA Polymerase) Amplify DNA target up to 6 kb Elongation velocity is 1kb/min Lacks of 3' to 5' exoneclease activity Makes 3'-dA overhangs PCR product, good for TA cloning 10x Reaction Buffer is included 

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DescriptionFast Pfu DNA Polymerase is a fast and highly efficient high fidelity DNA polymerase with 5 ′ -3 ′ DNA polymerase activity and 3 ′ -5 ′ exonuclease activity. This enzyme is modified from other high fidelity enzymes, with strong amplification ability, fast amplification speed, and high fidelity. It overcomes the shortcomings of poor amplification ability, low yield, and slow amplificationspeed of ordinary Pfu enzymes, greatly shortening the reaction time. The amplification efficiency of this product is 3-5kb/min. This product can be used for long fragment amplification and amplification of various complex templates. The 3 'end of the PCR product obtained from amplification does not contain the "A" base and can be directly cloned into a blunt end vector. If T/A cloning is required, "A" needs to be added to the end of the PCR product for cloning. This product is suitable for gene cloning, gene site directed mutagenesis, SNP and other amplification experiments. Definition of ActivityThe amount of enzyme required to incorporate 10nmol of deoxyribonucleotides into acidic insoluble substances within 30 minutes at 74℃ is defined as 1 active unit (U). Quality controlAfter multiple column purifications, SDS-PAGE analysis showed that its purity was greater than 98%; No exogenous nuclease activity detected; After being stored at room temperature for one month, there was no significant change in activity. Storage buffer [PH8.0] 20mM Tris HCl, 100mM KCl, 0.1mM EDTA, 0.1% NP-40 (v/v), 0.1% Tween-20 (v/v), 1mM DTT, 50% glycerol (v/v). Shipping and Storage-20°C

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SuperHiFi™ High Fidelity DNA Polymerase Features Exceptionally high fidelity – Over 50-fold better fidelity than Taq DNA Polymerase, eqivalent to Phusion polymerase Extremely efficient – With extension speed of 3-4kb/min, significantly shorter extension time  Robust performance – Amplification of long templates (up to15 kb) PCR products have blunt end  

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DNA Polymerase I Large (Klenow) Fragment is the large fragment of E. coli DNA Polymerase I. The Klenow Fragment retains the DNA-dependent DNA polymerase activity of the E. coli DNA Polymerase I but lacks the 5’→3’ exonuclease activity. With its inherent 3’→5’ exonuclease activity, Klenow possesses the polymerization fidelity of the holoenzyme without degrading 5'-termini. Applications: DNA blunting by filling-in 5'-overhangs with unlabeled or labeled dNTPs cDNA second-strand synthesis Generate single-stranded DNA probes using random primers Site-directed DNA mutagenesis using synthetic oligonucleotides Dideoxy DNA sequencing of single- or double-stranded DNA templates 3’→5’ exonuclease activity can blunt a 3’-overhang Concentration: 5 U/μl Storage: Store all components at -20°C. Storage Buffer: 25 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, and 50% (v/v) Glycerol. Note: One unit is defined as the amount of DNA Polymerase I Large (Klenow) Fragment that catalyzes the incorporation of 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C using poly(dA-dT):poly(dA-dT) as a template:primer. This product is distributed for laboratory research only. Caution: Not for diagnostic use.

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2x Taq PCR Premix is an optimized ready-to-use solution containing Taq DNA polymerase, standard Taq reaction buffer, dNTPs, tracking dyes and stabilizers. It is ideally suited to routine PCR applications such as sub-cloning, colony screening and genotyping. It can amplify up to 4 kb from complex genomic DNA or up to 5 kb from lambda DNA. Tracking dyes included in the mix: Blue dye migrates approximately 4 kb, and yellow dye migrates approximately 50 bp. ***** This is a cold item. You are required to select Overnight shipping for purchasing this item! *****

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2x Taq PCR Premix is an optimized ready-to-use solution containing Taq DNA polymerase, standard Taq reaction buffer, dNTPs and stabilizers. It is ideally suited to routine PCR applications such as sub-cloning, colony screening and genotyping. It can amplify up to 4 kb from complex genomic DNA or up to 5 kb from lambda DNA.  No tracking dye included in the mix. 20x1ml, contains 1000U Taq DNA polymerase ***** This is a cold item. You are requires to select Overnight shipping for purchase this item! *****

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An optimized ready-to-use solution containing Hot Start Taq DNA polymerase, standard Hot Start Taq reaction buffer, dNTPs, tracking dye and stabilizers. It is ideally suited to routine PCR applications such as sub-cloning, colony screening and genotyping. It can amplify up to 6 kb from complex genomic DNA. Blue dye migrates approximately at 4 kb. 10x1 ml. Storage: -20ºC ***** This is a cold item. You are requires to select Overnight shipping for purchase this item! *****

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General description Betaine also called trimethylglycine or N,N,N triethylammonium acetate, is an analog of glycine with three methyl groups. It is highly compatible with polymerase chain reaction (PCR) buffer mixture. Betaine is a PCR enhancing reagent that is widely used for improving the yield and specificity of PCR products, especially during the PCR amplification of targets rich in GC content or those that form secondary structures resulting in poor yield. Betaine facilitates DNA strand separation and manages the DNA melting temperature (Tm) difference between the GC and AT pairs in DNA. It stabilizes the dsDNA by equalizing the contribution of GC- and AT-base pairs. Betaine has been broadly used to optimize multiplex and ‘long and accurate’ polymerase chain reaction (LA-PCR). The addition of 1-2 M aqueous betaine to a PCR mixture has been reported to reduce the base pair composition dependence on DNA strand melting.  Application Betaine solution has been used as a component of loop-mediated isothermal amplification (LAMP) reactions polymerase chain reaction for genomic DNA amplification quantitative polymerase chain reaction (qPCR) for telomere length analysis in the PCR amplification of CGG repeats in the genomic DNA. Final Reaction Concentration: Most protocols use 1.0 M to 1.5 M as a starting point, which can be optimized up to 2.0 M for particularly difficult, GC-rich targets or templates that form tight secondary structures. Volume Calculation (using a 5M stock solution): If you are setting up a standard 20ul or 50ul PCR reaction, you will add enough of your 5M betaine solution to hit that final 1 M–2 M concentration. For example: To get 1.0 M final concentration in a 20ul reaction, you need to add 4ul of a 5M betaine stock. To get 1.5 M final concentration in a 20ul reaction, you need to add 6ul of a 5M betaine stock. Storage:  Short-Term Storage: Store at 4oC (refrigerated) for daily or weekly use. Long-Term Storage: Aliquot the solution into smaller volumes and store at -20oC to prevent contamination and repeated freeze-thaw cycles. Shipping: 5M betaine is a stable aqueous solution and does not require cold-chain (ice or dry ice) shipping. It can be shipped at ambient room temperature.

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