5 U/µl (1µl contains 5 units Taq DNA Polymerase) Amplify DNA target up to 6 kb Elongation velocity is 1kb/min Lacks of 3' to 5' exoneclease activity Makes 3'-dA overhangs PCR product, good for TA cloning 10x Reaction Buffer is included 

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PowerPCR™ Pfu DNA polymerase is a highly thermostable DNA polymerase from the hyperthermophilic archaeum Pyrococcus furiosus. 2.5 U/µl  Amplify DNA target up to 2 kb The elongation velocity is 0.2~0.4kb/min (70~75°C). 6-10 fold higher fidelity than Taq DNA polymerase Highly thermostable – remains 95% active after 2 hours incubation at 95°C Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides) Possesses 3' to 5' exonuclease proofreading activity Generates in blunt-ended PCR products Applications High fidelity PCR Generation of PCR products for cloning and expression RT-PCR for cDNA cloning and expression Blunt-end PCR cloning

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SuperHiFi™ High Fidelity DNA Polymerase Features Exceptionally high fidelity – Over 50-fold better fidelity than Taq DNA Polymerase, eqivalent to Phusion polymerase Extremely efficient – With extension speed of 3-4kb/min, significantly shorter extension time  Robust performance – Amplification of long templates (up to15 kb) PCR products have blunt end  

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DNA Polymerase I Large (Klenow) Fragment is the large fragment of E. coli DNA Polymerase I. The Klenow Fragment retains the DNA-dependent DNA polymerase activity of the E. coli DNA Polymerase I but lacks the 5’→3’ exonuclease activity. With its inherent 3’→5’ exonuclease activity, Klenow possesses the polymerization fidelity of the holoenzyme without degrading 5'-termini. Applications: DNA blunting by filling-in 5'-overhangs with unlabeled or labeled dNTPs cDNA second-strand synthesis Generate single-stranded DNA probes using random primers Site-directed DNA mutagenesis using synthetic oligonucleotides Dideoxy DNA sequencing of single- or double-stranded DNA templates 3’→5’ exonuclease activity can blunt a 3’-overhang Concentration: 5 U/μl Storage: Store all components at -20°C. Storage Buffer: 25 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, and 50% (v/v) Glycerol. Note: One unit is defined as the amount of DNA Polymerase I Large (Klenow) Fragment that catalyzes the incorporation of 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C using poly(dA-dT):poly(dA-dT) as a template:primer. This product is distributed for laboratory research only. Caution: Not for diagnostic use.

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2x Taq PCR Premix is an optimized ready-to-use solution containing Taq DNA polymerase, standard Taq reaction buffer, dNTPs, tracking dyes and stabilizers. It is ideally suited to routine PCR applications such as sub-cloning, colony screening and genotyping. It can amplify up to 4 kb from complex genomic DNA or up to 5 kb from lambda DNA. Tracking dyes included in the mix: Blue dye migrates approximately 4 kb, and yellow dye migrates approximately 50 bp. ***** This is a cold item. You are requires to select Overnight shipping for purchase this item! *****

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2x Taq PCR Premix is an optimized ready-to-use solution containing Taq DNA polymerase, standard Taq reaction buffer, dNTPs and stabilizers. It is ideally suited to routine PCR applications such as sub-cloning, colony screening and genotyping. It can amplify up to 4 kb from complex genomic DNA or up to 5 kb from lambda DNA.  No tracking dye included in the mix. 20x1ml, contains 1000U Taq DNA polymerase

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An optimized ready-to-use solution containing Hot Start Taq DNA polymerase, standard Hot Start Taq reaction buffer, dNTPs, tracking dye and stabilizers. It is ideally suited to routine PCR applications such as sub-cloning, colony screening and genotyping. It can amplify up to 6 kb from complex genomic DNA. Blue dye migrates approximately at 4 kb. 10x1 ml. Storage: -20ºC ***** This is a cold item. You are requires to select Overnight shipping for purchase this item! *****

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