Description
Fast Pfu DNA Polymerase is a fast and highly efficient high fidelity DNA polymerase with 5 ′ -3 ′ DNA polymerase activity and 3 ′ -5 ′ exonuclease activity. This enzyme is modified from other high fidelity enzymes, with strong amplification ability, fast amplification speed, and high fidelity. It overcomes the shortcomings of poor amplification ability, low yield, and slow amplification
speed of ordinary Pfu enzymes, greatly shortening the reaction time. The amplification efficiency of this product is 3-5kb/min.
This product can be used for long fragment amplification and amplification of various complex templates. The 3 'end of the PCR product obtained from amplification does not contain the "A" base and can be directly cloned into a blunt end vector. If T/A cloning is required, "A" needs to be added to the end of the PCR product for cloning. This product is suitable for gene cloning, gene site directed mutagenesis, SNP and other amplification experiments.
Definition of Activity
The amount of enzyme required to incorporate 10nmol of deoxyribonucleotides into acidic insoluble substances within 30 minutes at 74℃ is defined as 1 active unit (U).
Quality control
After multiple column purifications, SDS-PAGE analysis showed that its purity was greater than 98%; No exogenous nuclease activity detected; After being stored at room temperature for one month, there was no significant change in activity.
Storage buffer
[PH8.0] 20mM Tris HCl, 100mM KCl, 0.1mM EDTA, 0.1% NP-40 (v/v), 0.1% Tween-20 (v/v), 1mM DTT, 50% glycerol (v/v).
Shipping and Storage
-20°C
