PowerPCR™ Pfu DNA polymerase is a highly thermostable DNA polymerase from the hyperthermophilic archaeum Pyrococcus furiosus.
- 2.5 U/µl
- Amplify DNA target up to 2 kb
- The elongation velocity is 0.2~0.4kb/min (70~75°C).
- 6-10 fold higher fidelity than Taq DNA polymerase
- Highly thermostable – remains 95% active after 2 hours incubation at 95°C
- Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides)
- Possesses 3' to 5' exonuclease proofreading activity
- Generates in blunt-ended PCR products
Applications
- High fidelity PCR
- Generation of PCR products for cloning and expression
- RT-PCR for cDNA cloning and expression
- Blunt-end PCR cloning