Pfu DNA Polymerase (2.5 U/µl)

Description

PowerPCR™ Pfu DNA polymerase is a highly thermostable DNA polymerase from the hyperthermophilic archaeum Pyrococcus furiosus.

  • 2.5 U/µl
  •  Amplify DNA target up to 2 kb
  • The elongation velocity is 0.2~0.4kb/min (70~75°C).
  • 6-10 fold higher fidelity than Taq DNA polymerase
  • Highly thermostable – remains 95% active after 2 hours incubation at 95°C
  • Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides)
  • Possesses 3' to 5' exonuclease proofreading activity
  • Generates in blunt-ended PCR products

Applications

  • High fidelity PCR
  • Generation of PCR products for cloning and expression
  • RT-PCR for cDNA cloning and expression
  • Blunt-end PCR cloning

Read more

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SKU: E03-01

Pfu DNA Polymerase (2.5 U/µl)

PowerPCR™ Pfu DNA polymerase is a highly thermostable DNA polymerase from the hyperthermophilic archaeum Pyrococcus furiosus. 2.5 U/µl  Amplify DNA... Read more

$50.00 Excl. VAT

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    Description

    PowerPCR™ Pfu DNA polymerase is a highly thermostable DNA polymerase from the hyperthermophilic archaeum Pyrococcus furiosus.

    • 2.5 U/µl
    •  Amplify DNA target up to 2 kb
    • The elongation velocity is 0.2~0.4kb/min (70~75°C).
    • 6-10 fold higher fidelity than Taq DNA polymerase
    • Highly thermostable – remains 95% active after 2 hours incubation at 95°C
    • Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides)
    • Possesses 3' to 5' exonuclease proofreading activity
    • Generates in blunt-ended PCR products

    Applications

    • High fidelity PCR
    • Generation of PCR products for cloning and expression
    • RT-PCR for cDNA cloning and expression
    • Blunt-end PCR cloning

    Read more

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