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Pfu DNA Polymerase (2.5 U/µl)

    Description

    PowerPCR™ Pfu DNA polymerase is a highly thermostable DNA polymerase from the hyperthermophilic archaeum Pyrococcus furiosus.

    • 2.5 U/µl
    •  Amplify DNA target up to 2 kb
    • The elongation velocity is 0.2~0.4kb/min (70~75°C).
    • 6-10 fold higher fidelity than Taq DNA polymerase
    • Highly thermostable – remains 95% active after 2 hours incubation at 95°C
    • Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides)
    • Possesses 3' to 5' exonuclease proofreading activity
    • Generates in blunt-ended PCR products

    Applications

    • High fidelity PCR
    • Generation of PCR products for cloning and expression
    • RT-PCR for cDNA cloning and expression
    • Blunt-end PCR cloning

    Read more

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    SKU: E03-01

    Pfu DNA Polymerase (2.5 U/µl)

      PowerPCR™ Pfu DNA polymerase is a highly thermostable DNA polymerase from the hyperthermophilic archaeum Pyrococcus furiosus. 2.5 U/µl  Amplify DNA... Read more

      $50.00

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          Description

          PowerPCR™ Pfu DNA polymerase is a highly thermostable DNA polymerase from the hyperthermophilic archaeum Pyrococcus furiosus.

          • 2.5 U/µl
          •  Amplify DNA target up to 2 kb
          • The elongation velocity is 0.2~0.4kb/min (70~75°C).
          • 6-10 fold higher fidelity than Taq DNA polymerase
          • Highly thermostable – remains 95% active after 2 hours incubation at 95°C
          • Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides)
          • Possesses 3' to 5' exonuclease proofreading activity
          • Generates in blunt-ended PCR products

          Applications

          • High fidelity PCR
          • Generation of PCR products for cloning and expression
          • RT-PCR for cDNA cloning and expression
          • Blunt-end PCR cloning

          Read more

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