3 μl or 5 μl per loading for clear visualization during electrophoresison 15-well or 10-well mini-gel, respectively.
1.5~2.5 μl per well for general Western transferring.
Apply more for thicker (> 1.5 mm) or larger gel.
The ladder is supplied in gel loading buffer and is ready to use. Do not heat, dilute, or add reducing agent before loading.
Description: The All Blue Prestained Protein Ladder I is a protein standard with 10 pre-stained proteins covering a wide range molecular weightsfor 10 to 180 kDa. Proteins are covalently coupled with a blue chromophore and two reference bands ( 25 kDa and 72 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). The All Blue Prestained Protein Ladder I is designed for monitoring protein separated during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximate sizing of proteins.
Contents: Approximately 0.2~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25°C), 2 % SDS, 1 mM 2-mercaptoethanol, 3.6M Urea, and 15 % (v/v) Glycerol).
Quality Control: Under suggested conditions, All Blue Prestained Protein Ladder I resolves10 major bands in polyacrylamide gel with appropriate buffers andafterWestern blotting to nitrocellulose membrane.
Storage:
Stable at 25°C for 2 weeks
Stable at 4°C for 3 months
Stable at -20°C for 24 months
Dual Color Prestained Protein Maker I is a prestained mixture of ten high-purity recombinant proteins (no His-tag). The bands display on Tris-Glycine gel range from 8-195 kDa (~8, ~13, ~20, ~28, ~40, ~55, ~70, ~105, ~140, ~195 kDa), of which 70 kDa and 8 kDa are rose-red bands, others are blue bands. which is convenient formonitoring of electrophoresis and transfer efficiency. It is suitable as a protein molecular weight standard forSDS-PAGE and Western Blot.
Shipping and Storage ConditionsShip with wet ice; Store at -20°C, valid for 12 months after receiving.
Dual Color Prestained Protein Maker I is a prestained mixture of ten high-purity recombinant proteins (no His-tag), The bands display on Tris-Glycine gel range from 8-195 kDa (~8, ~13, ~20, ~28, ~40, ~55, ~70, ~105, ~140, ~195 kDa), of which 70 kDa and 8 kDa are rose-red bands, others are blue bands. which is convenient for monitoring of electrophoresis and transfer efficiency. It is suitable as a protein molecular weight standard for SDS-PAGE and Western Blot.
Shipping and Storage ConditionsShip with wet ice; Store at -20°C, valid for 12 months after receiving.
Dual Color Prestained Protein Maker III consists of 11 highly purified and pre-stained recombinant proteins. The indicated molecular weight range in Tris-Glycine Gel was 10-200 kDa (~10, ~15, ~20, ~30, ~40, ~55, ~65, ~90, ~110, ~140, ~200 kDa). With orange-red bands at 90 and 30 kDa. rose-red band at 10 kDa, blue bands at the others. Therefore, it is convenient to observe the electrophoretic state of protein dynamically or judge the effect of protein transmembrane. It is suitable as a protein molecular weight standard for SDS-PAGE and Western blot.
His-Tagged proteins: Proteins 40, ~90, ~110, ~140, ~200 kDa are His-tagged. When blotted with anti-His antibody, most of the bands show up after ECL. 40 kDa band is weak, 90 kDa band doesn't show. This is very helpful when the protein marker is used in His-tagged protein purification.
Shipping and Storage ConditionsShip with wet ice; Store at -20°C, valid for 12 months after receiving.
Dual Color Prestained Protein Maker III consists of 11 highly purified and pre-stained recombinant proteins. The indicated molecular weight range in Tris-Glycine Gel was 10-200 kDa (~10, ~15, ~20, ~30, ~40, ~55, ~65, ~90, ~110, ~140, ~200 kDa). With orange-red bands at 90 and 30 kDa. rose-red band at 10 kDa, blue bands at the others. Therefore, it is convenient to observe the electrophoretic state of protein dynamically or judge the effect of protein transmembrane. It is suitable as a protein molecular weight standard for SDS-PAGE and Western blot.
His-Tagged proteins: Proteins 40, ~90, ~110, ~140, ~200 kDa are His-tagged. When blotted with anti-His antibody, most of the bands show up after ECL. 40 kDa band is weak, 90 kDa band doesn't show. This is very helpful when the protein marker is used in His-tagged protein purification.
Shipping and Storage ConditionsShip with wet ice; Store at -20°C, valid for 12 months after receiving.
Dual Color Prestained Protein Maker V consists of 8 high-purity and prestained recombinant proteins, which is convenient for monitoring of electrophoresis and transfer efficiency. It is suitable as a protein molecular weight standard for SDS-PAGE and Western Blot. The bands display in Tris-Glycine gel range from 55-320 kDa (~55, ~65, ~90, ~130, ~165, ~210, ~270, ~320 kDa), With orange-red band at 90 kDa, and blue bands at the others.
His-Tagged proteins: All the proteins are His-tagged. When blotted with anti-His antibody, most of the bands show up after ECL. 55kDa and 68 kDa bands are weak, 90 kDa band not showing. This is very helpful when the protein marker is used in His-tagged protein purification.
Shipping and Storage ConditionsShip with wet ice; Store at -20°C, valid for 12 months after receiving.
DescriptionDual Color Prestained Protein Maker V consists of 8 high-purity and prestained recombinant proteins, which is convenient for monitoring of electrophoresis and transfer efficiency. It is suitable as a protein molecular weight standard for SDS-PAGE and Western Blot. The bands display in Tris-Glycine gel range from 55-320 kDa (~55, ~65, ~90, ~130, ~165, ~210, ~270, ~320 kDa), With orange-red band at 90 kDa, and blue bands at the others.
His-Tagged proteins: All the proteins are His-tagged. When blotted with anti-His antibody, most of the bands show up after ECL. 55kDa and 68 kDa bands are weak, 90 kDa band not showing. This is very helpful when the protein marker is used in His-tagged protein purification.
Shipping and Storage ConditionsShip with wet ice; Store at -20°C, valid for 12 months after receiving.
Developable Prestained Protein Maker VI is composed of 8 kinds of high purity recombinant proteins and 4 kinds of predyed recombinant proteins.The indicated molecular weight of the protein ranges from 13 to 154 kDa (~13, 22, 28, ~28, 38, 50, 62, ~70, 78, 113, ~140, 154 kDa). Among them, 70 kDa is rose red prestain band, 13, 28, 140 kDa is blue prestain band, which is convenient to dynamically observe the state of protein electrophoresis or judge the effect of protein transfer. 8 recombinant proteins, including 22, 28, 38, 50, 62, 78, 113, 154 kDa, can bind almost all types of antibodies (except chicken antibodies), and can bind antibodies with the target protein at the same time and by ECL orother means of color development.
Shipping and Storage ConditionsShip with wet ice; Store at -20°C, valid for 12 months after receiving.
Developable Prestained Protein Maker VI is composed of 8 kinds of high purity recombinant proteins and 4 kinds of predyed recombinant proteins.The indicated molecular weight of the protein ranges from 13 to 154 kDa (~13, 22, 28, ~28, 38, 50, 62, ~70, 78, 113, ~140, 154 kDa). Among them, 70 kDa is rose red prestain band, 13, 28, 140 kDa is blue prestain band, which is convenient to dynamically observe the state of protein electrophoresis or judge the effect of protein transfer. 8 recombinant proteins, including 22, 28, 38, 50, 62, 78, 113, 154 kDa, can bind almost all types of antibodies (except chicken antibodies), and can bind antibodies with the target protein at the same time and by ECL or other means of color development.
Shipping and Storage ConditionsShip with wet ice; Store at -20°C, valid for 12 months after receiving.
Developable Prestained Protein Maker VI is a prestained, visualizable mixture of 7 high-purity recombinant proteins and 3 prestained recombinant proteins. The bands display in Tris-Glycine gel range from 30-209 kDa (~30, ~38, ~50, ~62, ~70, 78, ~105, ~113, ~154, ~209 kDa), of which 70 kDa is the red pre-stained band, 30 kDa and 105 kDa are the blue pre-stained bands to monitor of electrophoresis and transfer efficiency. The seven recombinant proteins (~38, ~50, ~62, ~78, ~113, ~154, ~209 kDa) can bind almost all types of antibodies (except chicken antibodies) and can bind antibodies simultaneously with the target protein and can be colourised by ECL or other means.
Shipping and Storage ConditionsShip with wet ice; Store at -20°C, valid for 12 months after receiving.
Developable Prestained Protein Maker VI is a prestained, visualizable mixture of 7 high-purity recombinant proteins and 3 prestained recombinant proteins. The bands display in Tris-Glycine gel range from 30-209 kDa (~30, ~38, ~50, ~62, ~70, 78, ~105, ~113, ~154, ~209 kDa), of which 70 kDa is the red pre-stained band, 30 kDa and 105 kDa are the blue pre-stained bands to monitor of electrophoresis and transfer efficiency. The seven recombinant proteins (~38, ~50, ~62, ~78, ~113, ~154, ~209 kDa) can bind almost all types of antibodies (except chicken antibodies) and can bind antibodies simultaneously with the target protein and can be colourised by ECL or other means.
Shipping and Storage ConditionsShip with wet ice; Store at -20°C, valid for 12 months after receiving.
