Tris-Glycine Native Sample Buffer (2/4/6x) is used to prepare protein samples for native gel electrophoresis using Tris-Glycine gels or Tris Acetate gels. It has a pH of 6.8 and contains bromophenol blue as a tracking dye. To use: Heat the sample in a 1x dilution (reduced or non-reduced) at 85°C for 2–5 minutes for optimal results. Note: Heating samples at 100°C in SDS-containing buffers results in proteolysis. 2x Composition: 200 mM Tris HCl, 20% Glycerol, 0.01% Bromophenol blue (Non-reduced). Add ß-mercaptoethanol (8% V/V) before use (Reduced).   2x MSDS 4x Composition: 400 mM Tris HCl, 40% Glycerol, 0.02% Bromophenol blue (Non-reduced). Add ß-mercaptoethanol (8% V/V) before use (Reduced).      4x MSDS 6x Composition: 600mM Tris.HCl, 50% glycerol, 0.02% bromophenol blue (Non-reduced). Add ß-mercaptoethanol (9% V/V) before use (Reduced).

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Tris-Glycine SDS Sample Buffer (2X) is used to prepare protein samples for denaturing gel electrophoresis using Tris-Glycine gels. It has a pH of 6.8 and contains bromophenol blue as a tracking dye. To use: Heat the sample in a 1X dilution (reduced or non-reduced) at 85°C for 2–5 minutes for optimal results. Note: Heating samples at 100°C in SDS-containing buffers results in proteolysis. 2x Composition: 125 mM Tris HCl, 4% SDS, 20% Glycerol, 0.01% Bromophenol blue (Non-reduced). Add ß-mercaptoethanol (8% V/V) before use (Reduced).   2x MSDS 4x Composition: 250 mM Tris HCl, 8% SDS, 40% Glycerol, 0.01% Bromophenol blue (Non-reduced). Add ß-mercaptoethanol (8% V/V) before use (Reduced).   4x MSDS 6x Composition: 375 mM Tris.HCl, 9% SDS, 50% glycerol, 0.015% bromophenol blue (Non-reduced). Add ß-mercaptoethanol (9% V/V) before use (Reduced). 6x MSDS

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