Tris-Glycine SDS Sample Buffer (2X) is used to prepare protein samples for denaturing gel electrophoresis using Tris-Glycine gels. It has a pH of 6.8 and contains bromophenol blue as a tracking dye.
To use: Heat the sample in a 1X dilution (reduced or non-reduced) at 85°C for 2–5 minutes for optimal results.
Note: Heating samples at 100°C in SDS-containing buffers results in proteolysis.
2x Composition: 125 mM Tris HCl, 4% SDS, 20% Glycerol, 0.01% Bromophenol blue (Non-reduced). Add ß-mercaptoethanol (8% V/V) before use (Reduced).
2x MSDS
4x Composition: 250 mM Tris HCl, 8% SDS, 40% Glycerol, 0.01% Bromophenol blue (Non-reduced). Add ß-mercaptoethanol (8% V/V) before use (Reduced).
4x MSDS
6x Composition: 375 mM Tris.HCl, 9% SDS, 50% glycerol, 0.015% bromophenol blue (Non-reduced). Add ß-mercaptoethanol (9% V/V) before use (Reduced).
6x MSDS
