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2x Tris-Glycine SDS Sample Buffer (25 ml)

    Description

    Tris-Glycine SDS Sample Buffer (2X) is used to prepare protein samples for denaturing gel electrophoresis using Tris-Glycine gels. It has a pH of 6.8 and contains bromophenol blue as a tracking dye.

    To use: Heat the sample in a 1X dilution (reduced or non-reduced) at 85°C for 2–5 minutes for optimal results.

    Note: Heating samples at 100°C in SDS-containing buffers results in proteolysis.

    Composition: 125 mM Tris HCl, 4% SDS, 20% Glycerol, 0.01% Bromophenol blue (Non-reduced). Add ß-mercaptoethanol (8% V/V) before use (Reduced).  

    MSDS

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    Product form

    SKU: SAB01-01

    2x Tris-Glycine SDS Sample Buffer (25 ml)

      Tris-Glycine SDS Sample Buffer (2X) is used to prepare protein samples for denaturing gel electrophoresis using Tris-Glycine gels. It has... Read more

      $25.00

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        Description

        Tris-Glycine SDS Sample Buffer (2X) is used to prepare protein samples for denaturing gel electrophoresis using Tris-Glycine gels. It has a pH of 6.8 and contains bromophenol blue as a tracking dye.

        To use: Heat the sample in a 1X dilution (reduced or non-reduced) at 85°C for 2–5 minutes for optimal results.

        Note: Heating samples at 100°C in SDS-containing buffers results in proteolysis.

        Composition: 125 mM Tris HCl, 4% SDS, 20% Glycerol, 0.01% Bromophenol blue (Non-reduced). Add ß-mercaptoethanol (8% V/V) before use (Reduced).  

        MSDS

        Read more

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