Washing Buffers

Name 1x Composition Applications
10x PBS Phosphate buffer 10 mM, KCl 2.7 mM, NaCl 137 mM, pH 7.4 Most widely used washing buffer for Western blot, ELISA, Immuno-Histochemistry staining et al.
10x PBST Phosphate buffer 10 mM, KCl 2.7 mM, NaCl 137 mM, Tween-20 0.05%, pH 7.4 Western blot, ELISA, Immuno-Histochemical staining et. al.
10x TBS TBS01: Tris 50 mM, NaCl 150 mM, pH 7.4 Western blot, ELISA, Immuno-Histochemistry
20x TBS TBS03, TBS04: Tris 25 mM, NaCl 137 mM, KCl 2.7 mM, pH 7.6 Western blot, ELISA, Immuno-Histochemistry
10x TBST TBST01: Tris 50 mM, NaCl 150 mM, Tween-20 0.05%, pH 7.4 Western blot, ELISA, Immuno-Histochemistry
20x TBST TBST03, TBST04: Tris 25mM, NaCl 137mM, KCl 2.7mM, Tween 0.05%, pH 7.6 Western blot, ELISA, Immuno-Histochemistry
14 products

  • 1x PBS Buffer (10 L)

    1x PBS Buffer (10 L)

    SKU: PBS02-10L

    PBS (phosphate buffered saline) is a pH-adjusted blend of ultrapure-grade phosphate buffers and saline solutions. It  contains 137 mM NaCl, 2.7 mM KCl, 8 mM Na2HPO4, and 2 mM KH2PO4. It is ready to use.  Product Specification Appearance Clear, Colorless Form Liquid pH 7.4 ± 0.2 Composition 137 mM NaCl, 2.7 mM KCl, 8 mM Na2HPO4, and 2 mM KH2PO4 Suitable For Western blot, ELISA, immunoprecipitation Comment All components are dissolved in ultrapure (type I) water, adjusted pH and filtered with 0.45µm filter Safety Data Sheet MSDS

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    $80.00

  • 1x PBST Buffer (10 L)

    1x PBST Buffer (10 L)

    SKU: PBST02-10L

    PBST (phosphate buffered saline) is a pH-adjusted blend of ultrapure-grade phosphate buffers and saline solutions. It contains 137 mM NaCl, 2.7 mM KCl, 10 mM phosphate buffer and 0.05% Tween-20. Tween 20 reduces nonspecific binding and protein: protein interactions. The solution is ready to use.  Product Specification Appearance Clear, Colorless Form Liquid pH 7.4 ± 0.2 Composition 137 mM NaCl, 2.7 mM KCl, 10mM phosphate buffer and 0.05% Tween-20 Suitable For Western blot, ELISA, immunoprecipitation Comment All components except Tween-20 are dissolved in ultrapure (type I) water, adjusted pH and filtered with 0.45µm filter. Tween-20 is added and mixed before shipment. Safety Data Sheet MSDS

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    $80.00

  • 10x PBS Buffer (1 L)

    10x PBS Buffer (1 L)

    SKU: PBS01-1L

    PBS (phosphate buffered saline) is a pH-adjusted blend of ultrapure-grade phosphate buffers and saline solutions which, when diluted to a 1X working concentration, contains 137 mM NaCl, 2.7 mM KCl, 8 mM Na2HPO4, and 2 mM KH2PO4. Each 10X PBS solution is ready to use upon dilution to the desired concentration.  Product Specification Appearance Clear, Colorless Form Liquid pH 7.4 ± 0.2, after 1X dilution with DI water Composition 1.37 M NaCl, 27 mM KCl, 80 mM Na2HPO4, and 20 mM KH2PO4 Suitable For Western blot, ELISA, immunoprecipitation Comment All components are dissolved in ultrapure (type I) water, adjusted pH and filtered with 0.45µm filter MSDS

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    $21.20

  • 10x PBST Buffer (1 L)

    10x PBST Buffer (1 L)

    SKU: PBST01-1L

    PBST (phosphate buffered saline with Tween-20) is a pH-adjusted blend of ultrapure-grade phosphate buffers and saline solutions which, when diluted to a 1X working concentration, contains 137 mM NaCl, 2.7 mM KCl, 10 mM phosphate buffer and 0.05% Tween-20. Tween 20 reduces nonspecific binding and protein: protein interactions. Each 10X PBST solution is ready to use upon dilution to the desired concentration.  Product Specification Appearance Clear, Colorless Form Liquid pH 7.4 ± 0.2, after 1X dilution with DI water Composition 1.37 M NaCl, 27 mM KCl, 100mM phosphate buffer and 0.5% Tween-20 Suitable For Western blot, ELISA, immunoprecipitation Comment All components except Tween-20 are dissolved in ultrapure (type I) water, adjusted pH and filtered with 0.45µm filter. Tween-20 is added and mixed before shipment. MSDS

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    $23.50

  • 10x PBS Buffer (4 L)

    10x PBS Buffer (4 L)

    SKU: PBS01-4L

    PBS (phosphate buffered saline) is a pH-adjusted blend of ultrapure-grade phosphate buffers and saline solutions which, when diluted to a 1X working concentration, contains 137 mM NaCl, 2.7 mM KCl, 8 mM Na2HPO4, and 2 mM KH2PO4. Each 10X PBS solution is ready to use upon dilution to the desired concentration.  Product Specification Appearance Clear, Colorless Form Liquid pH 7.4 ± 0.2, after 1X dilution with DI water Composition 1.37 M NaCl, 27 mM KCl, 80 mM Na2HPO4, and 20 mM KH2PO4 Suitable For Western blot, ELISA, immunoprecipitation Comment All components are dissolved in ultrapure (type I) water, adjusted pH and filtered with 0.45µm filter MSDS

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    $70.60

  • 10x PBST Buffer (4 L)

    10x PBST Buffer (4 L)

    SKU: PBST01-4L

    PBST (phosphate buffered saline with Tween-20) is a pH-adjusted blend of ultrapure-grade phosphate buffers and saline solutions which, when diluted to a 1X working concentration, contains 137 mM NaCl, 2.7 mM KCl, 10 mM phosphate buffer and 0.05% Tween-20. Tween 20 reduces nonspecific binding and protein: protein interactions. Each 10X PBST solution is ready to use upon dilution to the desired concentration.  Product Specification Appearance Clear, Colorless Form Liquid pH 7.4 ± 0.2, after 1X dilution with DI water Composition 1.37 M NaCl, 27 mM KCl, 100mM phosphate buffer and 0.5% Tween-20 Suitable For Western blot, ELISA, immunoprecipitation Comment All components except Tween-20 are dissolved in ultrapure (type I) water, adjusted pH and filtered with 0.45µm filter. Tween-20 is added and mixed before shipment. MSDS

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    $76.50

  • 10x PBS Buffer (10 L)

    10x PBS Buffer (10 L)

    SKU: PBS01-10L

    PBS (phosphate buffered saline) is a pH-adjusted blend of ultrapure-grade phosphate buffers and saline solutions which, when diluted to a 1X working concentration, contains 137 mM NaCl, 2.7 mM KCl, 8 mM Na2HPO4, and 2 mM KH2PO4. Each 10X PBS solution is ready to use upon dilution to the desired concentration.  Product Specification Appearance Clear, Colorless Form Liquid pH 7.4 ± 0.2, after 1X dilution with DI water Composition 1.37 M NaCl, 27 mM KCl, 80 mM Na2HPO4, and 20 mM KH2PO4 Suitable For Western blot, ELISA, immunoprecipitation Comment All components are dissolved in ultrapure (type I) water, adjusted pH and filtered with 0.45µm filter MSDS

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    $141.20

  • 10x PBST Buffer (10 L)

    10x PBST Buffer (10 L)

    SKU: PBST01-10L

    PBST (phosphate buffered saline with Tween-20) is a pH-adjusted blend of ultrapure-grade phosphate buffers and saline solutions which, when diluted to a 1X working concentration, contains 137 mM NaCl, 2.7 mM KCl, 10 mM phosphate buffer and 0.05% Tween-20. Tween 20 reduces nonspecific binding and protein: protein interactions. Each 10X PBST solution is ready to use upon dilution to the desired concentration.  Product Specification Appearance Clear, Colorless Form Liquid pH 7.4 ± 0.2, after 1X dilution with DI water Composition 1.37 M NaCl, 27 mM KCl, 100mM phosphate buffer and 0.5% Tween-20 Suitable For Western blot, ELISA, immunoprecipitation Comment All components except Tween-20 are dissolved in ultrapure (type I) water, adjusted pH and filtered with 0.45µm filter. Tween-20 is added and mixed before shipment. MSDS

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    $164.70

  • 10x TBS Buffer (10 L)

    10x TBS Buffer (10 L)

    SKU: TBS01-10L

    TRIS-buffered saline (10x TBS) is used as a washing buffer for Western blot and other alkaline phosphatase and peroxidase conjugates assay, such as ELISA and dot blot assay. It acts as a pH stabilizer, which enable washing without disruption of antibody-antigen binding interactions. Each 10X TBS solution is ready to use upon dilution to the desired concentration.  Product Specification Appearance Clear, Colorless Form Liquid pH 7.4 ± 0.2 Composition (1x) 50mM Tris, 150mM NaCl Comment All components are dissolved in ultrapure (Type I) water, followed by pH adjustment and filtered through 0.45 micron filter. MSDS

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    $129.40

  • 10xTBST Buffer (10 L)

    10xTBST Buffer (10 L)

    SKU: TBST01-10L

    TRIS-buffered saline with Tween-20 (10x TBST) is used as a washing buffer for Western blot and other immunoassays, such as ELISA and dot blot assay. It acts as a pH stabilizer, which enable washing without disruption of antibody-antigen binding interactions. Tween 20 reduces nonspecific binding and protein: protein interactions. Each 10X TBST solution is ready to use upon dilution to the desired concentration.  Product Specification Appearance Clear, Colorless Form Liquid pH 7.4 ± 0.2 Composition (1x) 50mM Tris, 150mM NaCl, 0.05% Tween-20 Comment All components except Tween-20 are dissolved in ultrapure (Type I) water, followed by pH adjustment and filtered through 0.45 micron filter. Tween-20 is added and mixed before shipment. MSDS

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    $141.20

  • 10x TBS Buffer (1 L)

    10x TBS Buffer (1 L)

    SKU: TBS01-1L

    TRIS-buffered saline (10x TBS) is used as a washing buffer for Western blot and other alkaline phosphatase and peroxidase conjugates assay, such as ELISA and dot blot assay. It acts as a pH stabilizer, which enable washing without disruption of antibody-antigen binding interactions. Each 10X TBS solution is ready to use upon dilution to the desired concentration.  Product Specification Appearance Clear, Colorless Form Liquid pH 7.4 ± 0.2 Composition (1x) 50mM Tris, 150mM NaCl Comment All components are dissolved in ultrapure (Type I) water, followed by pH adjustment and filtered through 0.45 micron filter. MSDS

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    $23.50

  • 10x TBS Buffer (4 L)

    10x TBS Buffer (4 L)

    SKU: TBS01-4L

    TRIS-buffered saline (10x TBS) is used as a washing buffer for Western blot and other alkaline phosphatase and peroxidase conjugates assay, such as ELISA and dot blot assay. It acts as a pH stabilizer, which enable washing without disruption of antibody-antigen binding interactions. Each 10X TBS solution is ready to use upon dilution to the desired concentration.  Product Specification Appearance Clear, Colorless Form Liquid pH 7.4 ± 0.2 Composition (1x) 50mM Tris, 150mM NaCl Comment All components are dissolved in ultrapure (Type I) water, followed by pH adjustment and filtered through 0.45 micron filter. MSDS

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    $68.20

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