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Klenow Fragment (5U/ul)

    Description

    DNA Polymerase I Large (Klenow) Fragment is the large fragment of E. coli DNA Polymerase I. The Klenow Fragment retains the DNA-dependent DNA polymerase activity of the E. coli DNA Polymerase I but lacks the 5’→3’ exonuclease activity. With its inherent 3’→5’ exonuclease activity, Klenow possesses the polymerization fidelity of the holoenzyme without degrading 5'-termini.

    Applications:

    • DNA blunting by filling-in 5'-overhangs with unlabeled or labeled dNTPs
    • cDNA second-strand synthesis
    • Generate single-stranded DNA probes using random primers
    • Site-directed DNA mutagenesis using synthetic oligonucleotides
    • Dideoxy DNA sequencing of single- or double-stranded DNA templates
    • 3’→5’ exonuclease activity can blunt a 3’-overhang

    Concentration: 5 U/μl

    Storage: Store all components at -20°C.

    Storage Buffer: 25 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, and 50% (v/v) Glycerol.

    Note:

    • One unit is defined as the amount of DNA Polymerase I Large (Klenow) Fragment that catalyzes the incorporation of 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C using poly(dA-dT):poly(dA-dT) as a template:primer.
    • This product is distributed for laboratory research only. Caution: Not for diagnostic use.
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    SKU: E13-01

    Klenow Fragment (5U/ul)

      DNA Polymerase I Large (Klenow) Fragment is the large fragment of E. coli DNA Polymerase I. The Klenow Fragment retains... Read more

      $70.00

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          Description

          DNA Polymerase I Large (Klenow) Fragment is the large fragment of E. coli DNA Polymerase I. The Klenow Fragment retains the DNA-dependent DNA polymerase activity of the E. coli DNA Polymerase I but lacks the 5’→3’ exonuclease activity. With its inherent 3’→5’ exonuclease activity, Klenow possesses the polymerization fidelity of the holoenzyme without degrading 5'-termini.

          Applications:

          • DNA blunting by filling-in 5'-overhangs with unlabeled or labeled dNTPs
          • cDNA second-strand synthesis
          • Generate single-stranded DNA probes using random primers
          • Site-directed DNA mutagenesis using synthetic oligonucleotides
          • Dideoxy DNA sequencing of single- or double-stranded DNA templates
          • 3’→5’ exonuclease activity can blunt a 3’-overhang

          Concentration: 5 U/μl

          Storage: Store all components at -20°C.

          Storage Buffer: 25 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, and 50% (v/v) Glycerol.

          Note:

          • One unit is defined as the amount of DNA Polymerase I Large (Klenow) Fragment that catalyzes the incorporation of 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C using poly(dA-dT):poly(dA-dT) as a template:primer.
          • This product is distributed for laboratory research only. Caution: Not for diagnostic use.

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