Formulated for running proteins on Bis-Tris gels, including Invitrogen's NuPAGE® and Bolt Bis-Tris gel. MOPS/SDS Running Buffer is preferred for separating medium- to large-sized proteins. Both MES SDS Running Buffer and MOPS SDS Running Buffer can be used with Bis-Tris gels. MES has a lower pKa than MOPS, making the MES SDS Running Buffer faster than the MOPS SDS running buffers. The difference in ion migration affects stacking and results in a difference in protein separation range between these buffers. Use of MOPS buffer allows proteins to run slower than when using MES buffer. MOPS buffer is preferred for separating medium- to large-sized proteins. MES buffer is preferred for separating small- to medium-sized proteins.Composition: Tris 50 mM, MOPS 50 mM, SDS 0.1%, EDTA 1 mM, pH 7.7. (Exact same composition as NuPage and Bolt 20xMOPS Running Buffers) Compatibility: Bis-Tris precast gels, including Bolt gels and NuPAGE gel Storage: 4°C recommended. Shelf-life: 6 months, when stored properly. MSDS

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Formulated for running proteins on Bis-Tris gels, including Invitrogen's NuPAGE® and Bolt Bis-Tris gel. MOPS/SDS Running Buffer is preferred for separating medium- to large-sized proteins. Both MES SDS Running Buffer and MOPS SDS Running Buffer can be used with Bis-Tris gels. MES has a lower pKa than MOPS, making the MES SDS Running Buffer faster than the MOPS SDS running buffers. The difference in ion migration affects stacking and results in a difference in protein separation range between these buffers. Use of MOPS buffer allows proteins to run slower than when using MES buffer. MOPS buffer is preferred for separating medium- to large-sized proteins. MES buffer is preferred for separating small- to medium-sized proteins.Composition: Tris 50 mM, MOPS 50 mM, SDS 0.1%, EDTA 1 mM, pH 7.7. (Exact same composition as NuPage and Bolt 20xMOPS Running Buffers) Compatibility: Bis-Tris precast gels, including Bolt gels and NuPAGE gel Storage: 4°C recommended. Shelf-life: 6 months, when stored properly. MSDS

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Formulated for running proteins on Bis-Tris gels, including Invitrogen's NuPAGE® and Bolt Bis-Tris gel. MOPS/SDS Running Buffer is preferred for separating medium- to large-sized proteins. Both MES SDS Running Buffer and MOPS SDS Running Buffer can be used with Bis-Tris gels. MES has a lower pKa than MOPS, making the MES SDS Running Buffer faster than the MOPS SDS running buffers. The difference in ion migration affects stacking and results in a difference in protein separation range between these buffers. Use of MOPS buffer allows proteins to run slower than when using MES buffer. MOPS buffer is preferred for separating medium- to large-sized proteins. MES buffer is preferred for separating small- to medium-sized proteins.Composition: Tris 50 mM, MOPS 50 mM, SDS 0.1%, EDTA 1 mM, pH 7.7. (Exact same composition as NuPage and Bolt 20xMOPS Running Buffers) Compatibility: Bis-Tris precast gels, including Bolt gels and NuPAGE gel Storage: 4°C recommended. Shelf-life: 6 months, when stored properly. MSDS

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Formulated for running proteins on Bis-Tris gels, including Invitrogen's NuPAGE® Bis-Tris gel. MES SDS Running Buffer is preferred for separating small- to medium-sized proteins. Composition: 1xMES SDS Running Buffer: Tris 50 mM, MES 50 mM, SDS 0.1%, EDTA 1 mM, pH 7.3. (Exact same composition as NuPage and Bolt 20xMES Running Buffers) Compatibility: Bis-Tris precast gels, including Bolt gels and NuPAGE gel Storage: 4°C recommended. Shelf-life: 6 months, when stored properly. MSDS

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Formulated for running proteins on Bis-Tris gels, including Invitrogen's NuPAGE® Bis-Tris gel. MES SDS Running Buffer is preferred for separating small- to medium-sized proteins. Composition: 1xMES SDS Running Buffer: Tris 50 mM, MES 50 mM, SDS 0.1%, EDTA 1 mM, pH 7.3. (Exact same composition as NuPage and Bolt 20xMES Running Buffers) Compatibility: Bis-Tris precast gels, including Bolt gels and NuPAGE gel Storage: 4°C recommended. Shelf-life: 6 months, when stored properly. MSDS

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Formulated for running proteins on Bis-Tris gels, including Invitrogen's NuPAGE® Bis-Tris gel. MES SDS Running Buffer is preferred for separating small- to medium-sized proteins. Composition: 1xMES SDS Running Buffer: Tris 50 mM, MES 50 mM, SDS 0.1%, EDTA 1 mM, pH 7.3. (Exact same composition as NuPage and Bolt 20xMES Running Buffers) Compatibility: Bis-Tris precast gels, including Bolt gels and NuPAGE gel Storage: 4°C recommended. Shelf-life: 6 months, when stored properly. MSDS

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20x Tris-Acetate SDS Running Buffer is formulated for separation of proteins in their denatured state on Tris-Acetate gels. Tris-Acetate gels provide excellent separation of large molecular weight proteins when used with Tris-Acetate SDS Running Buffer, and LDS Sample Buffer. Tris-Acetate gels can also be run with Tris-Glycine Native Running Buffer and sample buffer to resolve native proteins more effectively than with the Tris-Glycine gel system. Composition 1x: Tris 50 mM, Tricine 50 mM, SDS 0.1%, pH 8.24 Storage 4°C for 1 year MSDS

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20x Tris-Acetate SDS Running Buffer is formulated for separation of proteins in their denatured state on Tris-Acetate gels. Tris-Acetate gels provide excellent separation of large molecular weight proteins when used with Tris-Acetate SDS Running Buffer, and LDS Sample Buffer. Tris-Acetate gels can also be run with Tris-Glycine Native Running Buffer and sample buffer to resolve native proteins more effectively than with the Tris-Glycine gel system. Composition 1x: Tris 50 mM, Tricine 50 mM, SDS 0.1%, pH 8.24 Storage 4°C for 1 year MSDS

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20x Tris-Acetate SDS Running Buffer is formulated for separation of proteins in their denatured state on Tris-Acetate gels. Tris-Acetate gels provide excellent separation of large molecular weight proteins when used with Tris-Acetate SDS Running Buffer, and LDS Sample Buffer. Tris-Acetate gels can also be run with Tris-Glycine Native Running Buffer and sample buffer to resolve native proteins more effectively than with the Tris-Glycine gel system. Composition 1x: Tris 50 mM, Tricine 50 mM, SDS 0.1%, pH 8.24 Storage 4°C for 1 year MSDS

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10xTris-Glycine Native Running Buffer is designed for protein gel electrophoresis under native running conditions with Tris-Glycine gels or Tris-Acetate gels. Tris-Glycine gels do not contain SDS and can be used to accurately separate both native and denatured proteins, depending upon the sample and running buffers used. To separate denatured proteins on Tris-Glycine gels, use Tris-Glycine SDS Sample Buffer and Tris-Glycine SDS Running Buffer. To separate native proteins use Tris-Glycine Native Sample Buffer and Tris-Glycine Native Running Buffer. Composition: 10xTGS: Tris 250 mM, Glycine 1.92 M, pH 8.3 Usage Recommendations To prepare 1X TG buffer, add 100 ml of 10X TG buffer to 900 ml of deionized water and mix well. 

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10xTris-Glycine Native Running Buffer is designed for protein gel electrophoresis under native running conditions with Tris-Glycine gels or Tris-Acetate gels. Tris-Glycine gels do not contain SDS and can be used to accurately separate both native and denatured proteins, depending upon the sample and running buffers used. To separate denatured proteins on Tris-Glycine gels, use Tris-Glycine SDS Sample Buffer and Tris-Glycine SDS Running Buffer. To separate native proteins use Tris-Glycine Native Sample Buffer and Tris-Glycine Native Running Buffer. Composition: 10xTGS: Tris 250 mM, Glycine 1.92 M, pH 8.3 Usage Recommendations To prepare 1X TG buffer, add 100 ml of 10X TG buffer to 900 ml of deionized water and mix well. 

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10xTris-Glycine Native Running Buffer is designed for protein gel electrophoresis under native running conditions with Tris-Glycine gels or Tris-Acetate gels. Tris-Glycine gels do not contain SDS and can be used to accurately separate both native and denatured proteins, depending upon the sample and running buffers used. To separate denatured proteins on Tris-Glycine gels, use Tris-Glycine SDS Sample Buffer and Tris-Glycine SDS Running Buffer. To separate native proteins use Tris-Glycine Native Sample Buffer and Tris-Glycine Native Running Buffer. Composition: 10xTGS: Tris 250 mM, Glycine 1.92 M, pH 8.3 Usage Recommendations To prepare 1X TG buffer, add 100 ml of 10X TG buffer to 900 ml of deionized water and mix well. 

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