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20x MOPS SDS Running Buffer (4 L)

    Description

    Formulated for running proteins on Bis-Tris gels, including Invitrogen's NuPAGE® and Bolt Bis-Tris gel. MOPS/SDS Running Buffer is preferred for separating medium- to large-sized proteins.

    Both MES SDS Running Buffer and MOPS SDS Running Buffer can be used with Bis-Tris gels. MES has a lower pKa than MOPS, making the MES SDS Running Buffer faster than the MOPS SDS running buffers. The difference in ion migration affects stacking and results in a difference in protein separation range between these buffers. Use of MOPS buffer allows proteins to run slower than when using MES buffer. MOPS buffer is preferred for separating medium- to large-sized proteins. MES buffer is preferred for separating small- to medium-sized proteins.

    Composition: Tris 50 mM, MOPS 50 mM, SDS 0.1%, EDTA 1 mM, pH 7.7. (Exact same composition as NuPage and Bolt 20xMOPS Running Buffers)

    Compatibility: Bis-Tris precast gels, including Bolt gels and NuPAGE gel

    Storage: 4°C recommended.

    Shelf-life: 6 months, when stored properly.

    MSDS

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    SKU: MOPS01-4L

    20x MOPS SDS Running Buffer (4 L)

      Formulated for running proteins on Bis-Tris gels, including Invitrogen's NuPAGE® and Bolt Bis-Tris gel. MOPS/SDS Running Buffer is preferred for separating medium-... Read more

      $230.00

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        Description

        Formulated for running proteins on Bis-Tris gels, including Invitrogen's NuPAGE® and Bolt Bis-Tris gel. MOPS/SDS Running Buffer is preferred for separating medium- to large-sized proteins.

        Both MES SDS Running Buffer and MOPS SDS Running Buffer can be used with Bis-Tris gels. MES has a lower pKa than MOPS, making the MES SDS Running Buffer faster than the MOPS SDS running buffers. The difference in ion migration affects stacking and results in a difference in protein separation range between these buffers. Use of MOPS buffer allows proteins to run slower than when using MES buffer. MOPS buffer is preferred for separating medium- to large-sized proteins. MES buffer is preferred for separating small- to medium-sized proteins.

        Composition: Tris 50 mM, MOPS 50 mM, SDS 0.1%, EDTA 1 mM, pH 7.7. (Exact same composition as NuPage and Bolt 20xMOPS Running Buffers)

        Compatibility: Bis-Tris precast gels, including Bolt gels and NuPAGE gel

        Storage: 4°C recommended.

        Shelf-life: 6 months, when stored properly.

        MSDS

        Read more

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