EasyPrep™ EndoFree Plasmid Midiprep Kit provides a simple, fast and cost effective method to purify plasmid DNAs from bacterial culture, when amount of the plasmid DNA needed is about 200 µg. The kit is especially convenient to use when multiple plasmids need to be purified at the same time, as the use of traditional commercial kits is time-consuming. Key to the kit is our proprietary DNA binding systems that allow the high efficient DNA binding  while proteins and other impurities are removed by wash buffer. Nucleic acids are easily eluted with Elution Buffer. Our proprietary endotoxin removal buffer, Buffer RET, is designed to remove endotoxin by a single washing step without tedious phase partitioning steps. The purified plasmid DNA, with endotoxin level less than 0.1 EU per µg of DNA, is ready for transfection of endotoxin sensitive cell lines and microinjections. Kit's Contents SKU PD03-21PD03-31 PD03-22PD03-32 PD03-23PD03-33 Midiprep DNA Columns 10 25 50 EasyFilter 10 (PD03-31) 25 (PD03-32) 50 (PD03-33) Buffer A1 30 ml 75 ml 150 ml Buffer B1 30 ml 75 ml 150 ml Buffer N3 15 ml 30 ml 60 ml Buffer RET 30 ml 75 ml 150 ml DNA Wash Buffer (5x)* 25 ml 50 ml 2x50 ml EndoFree Elution Buffer 10 ml 20 ml 30 ml RNase A 110 µl 250 µl 500 ul Manual 1 1 1 * Add ethanol to washing buffer before use

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EasyPrep™ EndoFree Plasmid Midiprep Kit provides a simple, fast and cost effective method to purify plasmid DNAs from bacterial culture, when amount of the plasmid DNA needed is about 200 µg. The kit is especially convenient to use when multiple plasmids need to be purified at the same time, as the use of traditional commercial kits is time-consuming. Key to the kit is our proprietary DNA binding systems that allow the high efficient DNA binding  while proteins and other impurities are removed by wash buffer. Nucleic acids are easily eluted with Elution Buffer. Our proprietary endotoxin removal buffer, Buffer RET, is designed to remove endotoxin by a single washing step without tedious phase partitioning steps. The purified plasmid DNA, with endotoxin level less than 0.1 EU per µg of DNA, is ready for transfection of endotoxin sensitive cell lines and microinjections. Kit's Contents SKU PD03-21PD03-31 PD03-22PD03-32 PD03-23PD03-33 Midiprep DNA Columns 10 25 50 EasyFilter 10 (PD03-31) 25 (PD03-32) 50 (PD03-33) Buffer A1 30 ml 75 ml 150 ml Buffer B1 30 ml 75 ml 150 ml Buffer N3 15 ml 30 ml 60 ml Buffer RET 30 ml 75 ml 150 ml DNA Wash Buffer (5x)* 25 ml 50 ml 2x50 ml EndoFree Elution Buffer 10 ml 20 ml 30 ml RNase A 110 µl 250 µl 500 ul Manual 1 1 1 * Add ethanol to washing buffer before use

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EasyPrep™ EndoFree Plasmid Midiprep-II Kit provides a simple, fast and cost effective method to purify plasmid DNAs from bacterial culture, when amount of the plasmid DNA needed is about 500 µg. The kit is especially convenient to use when multiple plasmids need to be purified at the same time, as the use of traditional commercial kits is time-consuming. Key to the kit is our proprietary DNA binding systems that allow the high efficient DNA binding  while proteins and other impurities are removed by wash buffer. Nucleic acids are easily eluted with Elution Buffer. Our proprietary endotoxin removal buffer, Buffer RET, is designed to remove endotoxin by a single washing step without tedious phase partitioning steps. The purified plasmid DNA, with endotoxin level less than 0.1 EU per µg of DNA, is ready for transfection of endotoxin sensitive cell lines and microinjections. Kit's Content SKU PD04-21PD04-31 PD04-22PD04-32 Midiprep-II DNA Columns 10 25 ezFilter 10 (PD04-31) 25 (PD04-32) Buffer A1 60 ml 140 ml Buffer A2 60 ml 140 ml Buffer N3 30 ml 70 ml Buffer KB 60 ml 140 ml Buffer RET 100 ml 250 ml DNA Wash Buffer (5x)* 50 ml 2x54 ml Column Buffer 35 ml 90 ml EndoFree Elution Buffer  15 ml 30 ml RNase A 220 µl 520 µl Manual 1 1 * Add ethanol to washing buffer before use

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EasyPrep™ EndoFree Plasmid Midiprep-II Kit provides a simple, fast and cost effective method to purify plasmid DNAs from bacterial culture, when amount of the plasmid DNA needed is about 500 µg. The kit is especially convenient to use when multiple plasmids need to be purified at the same time, as the use of traditional commercial kits is time-consuming. Key to the kit is our proprietary DNA binding systems that allow the high efficient DNA binding  while proteins and other impurities are removed by wash buffer. Nucleic acids are easily eluted with Elution Buffer. Our proprietary endotoxin removal buffer, Buffer RET, is designed to remove endotoxin by a single washing step without tedious phase partitioning steps. The purified plasmid DNA, with endotoxin level less than 0.1 EU per µg of DNA, is ready for transfection of endotoxin sensitive cell lines and microinjections. Kit's Content SKU PD04-21PD04-31 PD04-22PD04-32 Midiprep-II DNA Columns 10 25 ezFilter 10 (PD04-31) 25 (PD04-32) Buffer A1 60 ml 140 ml Buffer A2 60 ml 140 ml Buffer N3 30 ml 70 ml Buffer KB 60 ml 140 ml Buffer RET 100 ml 250 ml DNA Wash Buffer (5x)* 50 ml 2x54 ml Column Buffer 35 ml 90 ml EndoFree Elution Buffer  15 ml 30 ml RNase A 220 µl 520 µl Manual 1 1 * Add ethanol to washing buffer before use

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Isolate up to 2 - 3 mg of high copy number plasmid DNA from 500 mL bacterial culture

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Isolate up to 2 - 3 mg of high copy number plasmid DNA from 500 mL bacterial culture

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Isolate up to 5-6 mg of high copy number plasmid DNA from 1000 mL bacterial culture

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Isolate up to 5-6 mg of high copy number plasmid DNA from 1000 mL bacterial culture

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Isolate up to 2 - 10 mg of high copy number plasmid DNA from 500 -2000 mL culture

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Isolate up to 10 mg of high copy number plasmid DNA from 2000 mL bacterial culture

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EasyPrep™ EndoFree Plasmid Maxiprep Kit provides a simple, fast and cost effective method to purify plasmid DNAs from bacterial culture, when amount of the plasmid DNA needed is about 1,000 µg. The kit is especially convenient to use when multiple plasmids need to be purified at the same time, as the use of traditional commercial kits is time-consuming. Key to the kit is our proprietary DNA binding systems that allow the high efficient DNA binding  while proteins and other impurities are removed by wash buffer. Nucleic acids are easily eluted with Elution Buffer. Our proprietary endotoxin removal buffer, Buffer RET, is designed to remove endotoxin by a single washing step without tedious phase partitioning steps. The purified plasmid DNA, with endotoxin level less than 0.1 EU per µg of DNA, is ready for transfection of endotoxin sensitive cell lines and microinjections. Kit's Contents SKU PD05-21PD05-31 PD05-22PD05-32 Maxiprep DNA Columns 10 25 EasyFilter 10 (PD05-31) 25 (PD05-32) Buffer A1 120 ml 270 ml Buffer A2 120 ml 270 ml Buffer N3 60 ml 140 ml Buffer KB 120 ml 270 ml Buffer RET 200 ml 500 ml DNA Wash Buffer (5x)* 50 ml 2x 50 ml Column Buffer 35 ml 90 ml Elution Buffer 20 ml 30 ml RNase A 420 µl 960 µl Manual 1 1 * Add ethanol to washing buffer before use

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EasyPrep™ EndoFree Plasmid Maxiprep Kit provides a simple, fast and cost effective method to purify plasmid DNAs from bacterial culture, when amount of the plasmid DNA needed is about 1,000 µg. The kit is especially convenient to use when multiple plasmids need to be purified at the same time, as the use of traditional commercial kits is time-consuming. Key to the kit is our proprietary DNA binding systems that allow the high efficient DNA binding  while proteins and other impurities are removed by wash buffer. Nucleic acids are easily eluted with Elution Buffer. Our proprietary endotoxin removal buffer, Buffer RET, is designed to remove endotoxin by a single washing step without tedious phase partitioning steps. The purified plasmid DNA, with endotoxin level less than 0.1 EU per µg of DNA, is ready for transfection of endotoxin sensitive cell lines and microinjections. Kit's Contents SKU PD05-21PD05-31 PD05-22PD05-32 Maxiprep DNA Columns 10 25 ezFilter 10(PD05-31) 25(PD05-32) Buffer A1 120 ml 270 ml Buffer B1 120 ml 270 ml Buffer N3 60 ml 150 ml Buffer RET 120 ml 270 ml DNA Wash Buffer (5x)* 54 ml 2x 54 ml EndoFree Elution Buffer 20 ml 30 ml RNase A 420 µl 920 µl Manual 1 1 * Add ethanol to washing buffer before use

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