EasyPrep™ EndoFree Plasmid Midiprep Kit provides a simple, fast and cost effective method to purify plasmid DNAs from bacterial culture, when amount of the plasmid DNA needed is about 200 µg. The kit is especially convenient to use when multiple plasmids need to be purified at the same time, as the use of traditional commercial kits is time-consuming. Key to the kit is our proprietary DNA binding systems that allow the high efficient DNA binding  while proteins and other impurities are removed by wash buffer. Nucleic acids are easily eluted with Elution Buffer. Our proprietary endotoxin removal buffer, Buffer RET, is designed to remove endotoxin by a single washing step without tedious phase partitioning steps. The purified plasmid DNA, with endotoxin level less than 0.1 EU per µg of DNA, is ready for transfection of endotoxin sensitive cell lines and microinjections. Kit's Contents SKU PD03-21PD03-31 PD03-22PD03-32 PD03-23PD03-33 Midiprep DNA Columns 10 25 50 EasyFilter 10 (PD03-31) 25 (PD03-32) 50 (PD03-33) Buffer A1 30 ml 75 ml 150 ml Buffer B1 30 ml 75 ml 150 ml Buffer N3 15 ml 30 ml 60 ml Buffer RET 30 ml 75 ml 150 ml DNA Wash Buffer (5x)* 25 ml 50 ml 2x50 ml EndoFree Elution Buffer 10 ml 20 ml 30 ml RNase A 110 µl 250 µl 500 ul Manual 1 1 1 * Add ethanol to washing buffer before use

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EasyPrep™ EndoFree Plasmid Midiprep Kit provides a simple, fast and cost effective method to purify plasmid DNAs from bacterial culture, when amount of the plasmid DNA needed is about 200 µg. The kit is especially convenient to use when multiple plasmids need to be purified at the same time, as the use of traditional commercial kits is time-consuming. Key to the kit is our proprietary DNA binding systems that allow the high efficient DNA binding  while proteins and other impurities are removed by wash buffer. Nucleic acids are easily eluted with Elution Buffer. Our proprietary endotoxin removal buffer, Buffer RET, is designed to remove endotoxin by a single washing step without tedious phase partitioning steps. The purified plasmid DNA, with endotoxin level less than 0.1 EU per µg of DNA, is ready for transfection of endotoxin sensitive cell lines and microinjections. Kit's Contents SKU PD03-21PD03-31 PD03-22PD03-32 PD03-23PD03-33 Midiprep DNA Columns 10 25 50 EasyFilter 10 (PD03-31) 25 (PD03-32) 50 (PD03-33) Buffer A1 30 ml 75 ml 150 ml Buffer B1 30 ml 75 ml 150 ml Buffer N3 15 ml 30 ml 60 ml Buffer RET 30 ml 75 ml 150 ml DNA Wash Buffer (5x)* 25 ml 50 ml 2x50 ml EndoFree Elution Buffer 10 ml 20 ml 30 ml RNase A 110 µl 250 µl 500 ul Manual 1 1 1 * Add ethanol to washing buffer before use

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EasyPrep™ EndoFree Plasmid Midiprep-II Kit provides a simple, fast and cost effective method to purify plasmid DNAs from bacterial culture, when amount of the plasmid DNA needed is about 500 µg. The kit is especially convenient to use when multiple plasmids need to be purified at the same time, as the use of traditional commercial kits is time-consuming. Key to the kit is our proprietary DNA binding systems that allow the high efficient DNA binding  while proteins and other impurities are removed by wash buffer. Nucleic acids are easily eluted with Elution Buffer. Our proprietary endotoxin removal buffer, Buffer RET, is designed to remove endotoxin by a single washing step without tedious phase partitioning steps. The purified plasmid DNA, with endotoxin level less than 0.1 EU per µg of DNA, is ready for transfection of endotoxin sensitive cell lines and microinjections. Kit's Content SKU PD04-21PD04-31 PD04-22PD04-32 Midiprep-II DNA Columns 10 25 ezFilter 10 (PD04-31) 25 (PD04-32) Buffer A1 60 ml 140 ml Buffer A2 60 ml 140 ml Buffer N3 30 ml 70 ml Buffer KB 60 ml 140 ml Buffer RET 100 ml 250 ml DNA Wash Buffer (5x)* 50 ml 2x54 ml Column Buffer 35 ml 90 ml EndoFree Elution Buffer  15 ml 30 ml RNase A 220 µl 520 µl Manual 1 1 * Add ethanol to washing buffer before use

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EasyPrep™ EndoFree Plasmid Midiprep-II Kit provides a simple, fast and cost effective method to purify plasmid DNAs from bacterial culture, when amount of the plasmid DNA needed is about 500 µg. The kit is especially convenient to use when multiple plasmids need to be purified at the same time, as the use of traditional commercial kits is time-consuming. Key to the kit is our proprietary DNA binding systems that allow the high efficient DNA binding  while proteins and other impurities are removed by wash buffer. Nucleic acids are easily eluted with Elution Buffer. Our proprietary endotoxin removal buffer, Buffer RET, is designed to remove endotoxin by a single washing step without tedious phase partitioning steps. The purified plasmid DNA, with endotoxin level less than 0.1 EU per µg of DNA, is ready for transfection of endotoxin sensitive cell lines and microinjections. Kit's Content SKU PD04-21PD04-31 PD04-22PD04-32 Midiprep-II DNA Columns 10 25 ezFilter 10 (PD04-31) 25 (PD04-32) Buffer A1 60 ml 140 ml Buffer A2 60 ml 140 ml Buffer N3 30 ml 70 ml Buffer KB 60 ml 140 ml Buffer RET 100 ml 250 ml DNA Wash Buffer (5x)* 50 ml 2x54 ml Column Buffer 35 ml 90 ml EndoFree Elution Buffer  15 ml 30 ml RNase A 220 µl 520 µl Manual 1 1 * Add ethanol to washing buffer before use

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EasyPrep™ EndoFree Plasmid Maxiprep Kit provides a simple, fast and cost effective method to purify plasmid DNAs from bacterial culture, when amount of the plasmid DNA needed is about 1,000 µg. The kit is especially convenient to use when multiple plasmids need to be purified at the same time, as the use of traditional commercial kits is time-consuming. Key to the kit is our proprietary DNA binding systems that allow the high efficient DNA binding  while proteins and other impurities are removed by wash buffer. Nucleic acids are easily eluted with Elution Buffer. Our proprietary endotoxin removal buffer, Buffer RET, is designed to remove endotoxin by a single washing step without tedious phase partitioning steps. The purified plasmid DNA, with endotoxin level less than 0.1 EU per µg of DNA, is ready for transfection of endotoxin sensitive cell lines and microinjections. Kit's Contents SKU PD05-21PD05-31 PD05-22PD05-32 Maxiprep DNA Columns 10 25 EasyFilter 10 (PD05-31) 25 (PD05-32) Buffer A1 120 ml 270 ml Buffer A2 120 ml 270 ml Buffer N3 60 ml 140 ml Buffer KB 120 ml 270 ml Buffer RET 200 ml 500 ml DNA Wash Buffer (5x)* 50 ml 2x 50 ml Column Buffer 35 ml 90 ml Elution Buffer 20 ml 30 ml RNase A 420 µl 960 µl Manual 1 1 * Add ethanol to washing buffer before use

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EasyPrep™ EndoFree Plasmid Maxiprep Kit provides a simple, fast and cost effective method to purify plasmid DNAs from bacterial culture, when amount of the plasmid DNA needed is about 1,000 µg. The kit is especially convenient to use when multiple plasmids need to be purified at the same time, as the use of traditional commercial kits is time-consuming. Key to the kit is our proprietary DNA binding systems that allow the high efficient DNA binding  while proteins and other impurities are removed by wash buffer. Nucleic acids are easily eluted with Elution Buffer. Our proprietary endotoxin removal buffer, Buffer RET, is designed to remove endotoxin by a single washing step without tedious phase partitioning steps. The purified plasmid DNA, with endotoxin level less than 0.1 EU per µg of DNA, is ready for transfection of endotoxin sensitive cell lines and microinjections. Kit's Contents SKU PD05-21PD05-31 PD05-22PD05-32 Maxiprep DNA Columns 10 25 ezFilter 10(PD05-31) 25(PD05-32) Buffer A1 120 ml 270 ml Buffer B1 120 ml 270 ml Buffer N3 60 ml 150 ml Buffer RET 120 ml 270 ml DNA Wash Buffer (5x)* 54 ml 2x 54 ml EndoFree Elution Buffer 20 ml 30 ml RNase A 420 µl 920 µl Manual 1 1 * Add ethanol to washing buffer before use

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EasyPrep™ EndoFree Plasmid Midiprep-II Kit provides a simple, fast and cost effective method to purify plasmid DNAs from bacterial culture, when amount of the plasmid DNA needed is about 500 µg. The kit is especially convenient to use when multiple plasmids need to be purified at the same time, as the use of traditional commercial kits is time-consuming. Key to the kit is our proprietary DNA binding systems that allow the high efficient DNA binding  while proteins and other impurities are removed by wash buffer. Nucleic acids are easily eluted with Elution Buffer. Our proprietary endotoxin removal buffer, Buffer RET, is designed to remove endotoxin by a single washing step without tedious phase partitioning steps. The purified plasmid DNA, with endotoxin level less than 0.1 EU per µg of DNA, is ready for transfection of endotoxin sensitive cell lines and microinjections. Kit's Content SKU PD04-21PD04-31 PD04-22PD04-32 Midiprep-II DNA Columns 10 25 ezFilter 10 (PD04-31) 25 (PD04-32) Buffer A1 60 ml 140 ml Buffer A2 60 ml 140 ml Buffer N3 30 ml 70 ml Buffer KB 60 ml 140 ml Buffer RET 100 ml 250 ml DNA Wash Buffer (5x)* 50 ml 2x54 ml Column Buffer 35 ml 90 ml EndoFree Elution Buffer  15 ml 30 ml RNase A 220 µl 520 µl Manual 1 1 * Add ethanol to washing buffer before use

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EasyPrep™ EndoFree Plasmid Midiprep-II Kit provides a simple, fast and cost effective method to purify plasmid DNAs from bacterial culture, when amount of the plasmid DNA needed is about 500 µg. The kit is especially convenient to use when multiple plasmids need to be purified at the same time, as the use of traditional commercial kits is time-consuming. Key to the kit is our proprietary DNA binding systems that allow the high efficient DNA binding  while proteins and other impurities are removed by wash buffer. Nucleic acids are easily eluted with Elution Buffer. Our proprietary endotoxin removal buffer, Buffer RET, is designed to remove endotoxin by a single washing step without tedious phase partitioning steps. The purified plasmid DNA, with endotoxin level less than 0.1 EU per µg of DNA, is ready for transfection of endotoxin sensitive cell lines and microinjections. Kit's Content SKU PD04-21PD04-31 PD04-22PD04-32 Midiprep-II DNA Columns 10 25 ezFilter 10 (PD04-31) 25 (PD04-32) Buffer A1 60 ml 140 ml Buffer A2 60 ml 140 ml Buffer N3 30 ml 70 ml Buffer KB 60 ml 140 ml Buffer RET 100 ml 250 ml DNA Wash Buffer (5x)* 50 ml 2x54 ml Column Buffer 35 ml 90 ml EndoFree Elution Buffer  15 ml 30 ml RNase A 220 µl 520 µl Manual 1 1 * Add ethanol to washing buffer before use

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EasyPrep™ EndoFree Plasmid Midiprep Kit provides a simple, fast and cost effective method to purify plasmid DNAs from bacterial culture, when amount of the plasmid DNA needed is about 200 µg. The kit is especially convenient to use when multiple plasmids need to be purified at the same time, as the use of traditional commercial kits is time-consuming. Key to the kit is our proprietary DNA binding systems that allow the high efficient DNA binding  while proteins and other impurities are removed by wash buffer. Nucleic acids are easily eluted with Elution Buffer. Our proprietary endotoxin removal buffer, Buffer RET, is designed to remove endotoxin by a single washing step without tedious phase partitioning steps. The purified plasmid DNA, with endotoxin level less than 0.1 EU per µg of DNA, is ready for transfection of endotoxin sensitive cell lines and microinjections. Kit's Contents SKU PD03-21PD03-31 PD03-22PD03-32 PD03-23PD03-33 Midiprep DNA Columns 10 25 50 EasyFilter 10 (PD03-31) 25 (PD03-32) 50 (PD03-33) Buffer A1 30 ml 75 ml 150 ml Buffer B1 30 ml 75 ml 150 ml Buffer N3 15 ml 30 ml 60 ml Buffer RET 30 ml 75 ml 150 ml DNA Wash Buffer (5x)* 25 ml 50 ml 2x50 ml EndoFree Elution Buffer 10 ml 20 ml 30 ml RNase A 110 µl 250 µl 500 ul Manual 1 1 1 * Add ethanol to washing buffer before use

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EasyPrep™ EndoFree Plasmid Midiprep Kit provides a simple, fast and cost effective method to purify plasmid DNAs from bacterial culture, when amount of the plasmid DNA needed is about 200 µg. The kit is especially convenient to use when multiple plasmids need to be purified at the same time, as the use of traditional commercial kits is time-consuming. Key to the kit is our proprietary DNA binding systems that allow the high efficient DNA binding  while proteins and other impurities are removed by wash buffer. Nucleic acids are easily eluted with Elution Buffer. Our proprietary endotoxin removal buffer, Buffer RET, is designed to remove endotoxin by a single washing step without tedious phase partitioning steps. The purified plasmid DNA, with endotoxin level less than 0.1 EU per µg of DNA, is ready for transfection of endotoxin sensitive cell lines and microinjections. Kit's Contents SKU PD03-21PD03-31 PD03-22PD03-32 PD03-23PD03-33 Midiprep DNA Columns 10 25 50 EasyFilter 10 (PD03-31) 25 (PD03-32) 50 (PD03-33) Buffer A1 30 ml 75 ml 150 ml Buffer B1 30 ml 75 ml 150 ml Buffer N3 15 ml 35 ml 70 ml Buffer RET 30 ml 75 ml 150 ml DNA Wash Buffer (5x)* 25 ml 50 ml 2x50 ml EndoFree Elution Buffer 10 ml 20 ml 30 ml RNase A 110 µl 250 µl 500 ul Manual 1 1 1 * Add ethanol to washing buffer before use

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EasyPrep™ EndoFree Plasmid Midiprep Kit provides a simple, fast and cost effective method to purify plasmid DNAs from bacterial culture, when amount of the plasmid DNA needed is about 200 µg. The kit is especially convenient to use when multiple plasmids need to be purified at the same time, as the use of traditional commercial kits is time-consuming. Key to the kit is our proprietary DNA binding systems that allow the high efficient DNA binding  while proteins and other impurities are removed by wash buffer. Nucleic acids are easily eluted with Elution Buffer. Our proprietary endotoxin removal buffer, Buffer RET, is designed to remove endotoxin by a single washing step without tedious phase partitioning steps. The purified plasmid DNA, with endotoxin level less than 0.1 EU per µg of DNA, is ready for transfection of endotoxin sensitive cell lines and microinjections. Kit's Contents SKU PD03-21PD03-31 PD03-22PD03-32 PD03-23PD03-33 Midiprep DNA Columns 10 25 50 EasyFilter 10 (PD03-31) 25 (PD03-32) 50 (PD03-33) Buffer A1 30 ml 75 ml 150 ml Buffer B1 30 ml 75 ml 150 ml Buffer N3 15 ml 35 ml 70 ml Buffer RET 30 ml 75 ml 150 ml DNA Wash Buffer (5x)* 25 ml 50 ml 2x50 ml EndoFree Elution Buffer 10 ml 20 ml 30 ml RNase A 110 µl 250 µl 500 ul Manual 1 1 1 * Add ethanol to washing buffer before use

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Key to the kit is our proprietary DNA binding systems that allow the high efficient reversible binding of DNA to the mini column while proteins and other impurities are removed by wash buffer. Nucleic acids are then eluted with sterile water or elution buffer. Plasmid isolated with traditional protocol normally contains high level of endotoxins (lipopolysaccharides or LPS). For transfection of endotoxin sensitive cell lines or microinjection, the endotoxins should be removed before the applications. The EasyPrep™ endofree system uses a specially formulated buffer that extracts the endotoxin from the plasmid DNA. Two rounds of extraction will reduce the endotoxin level to 0.1 EU (Endotoxin) per µg of plasmid DNA. The endofree plasmid miniprep kit provides an efficient endotoxin removal step into the traditional purification procedure to produce transfection grade plasmid DNA. This kit is designed for fast and efficient purification of plasmid DNA from 3 to 10 mL of E. coli culture. The mini column II has a DNA binding capacity of 80 µg. The purified endofree DNA is ready for downstream applications such as transfection of endotoxin-sensitive cell lines, primary cultured cells or microinjection. Kit's Contents SKU PD02-11 PD02-12 Preps 50 250 DNA Miniprep-II Columns 50 250 Buffer A1 25 mL 125 mL Buffer A2 25 mL 125 mL Buffer N3 5 mL 25 mL Buffer KB 30 mL 135 mL Buffer RET 50 mL 250 mL DNA Wash Buffer* 20 mL 3 x 24 mL EndoFree Elution Buffer 10 mL 30 mL RNase A 175 μL 625 μL User Manual 1 1 * Add ethanol to washing buffer before use  

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