Key to the kit is our proprietary DNA binding systems that allow the high efficient reversible binding of DNA to the mini column while proteins and other impurities are removed by wash buffer. Nucleic acids are then eluted with sterile water or elution buffer.
Plasmid isolated with traditional protocol normally contains high level of endotoxins (lipopolysaccharides or LPS). For transfection of endotoxin sensitive cell lines or microinjection, the endotoxins should be removed before the applications. The EasyPrep™ endofree system uses a specially formulated buffer that extracts the endotoxin from the plasmid DNA. Two rounds of extraction will reduce the endotoxin level to 0.1 EU (Endotoxin) per µg of plasmid DNA. The endofree plasmid miniprep kit provides an efficient endotoxin removal step into the traditional purification procedure to produce transfection grade plasmid DNA.
This kit is designed for fast and efficient purification of plasmid DNA from 3 to 10 mL of E. coli culture. The mini column II has a DNA binding capacity of 80 µg. The purified endofree DNA is ready for downstream applications such as transfection of endotoxin-sensitive cell lines, primary cultured cells or microinjection.
Kit's Contents
| SKU |
PD02-11 |
PD02-12 |
| Preps |
50 |
250 |
| DNA Miniprep-II Columns |
50 |
250 |
| Buffer A1 |
25 mL |
125 mL |
| Buffer A2 |
25 mL |
125 mL |
| Buffer N3 |
5 mL |
25 mL |
| Buffer KB |
30 mL |
135 mL |
| Buffer RET |
50 mL |
250 mL |
| DNA Wash Buffer* |
20 mL |
3 x 24 mL |
| EndoFree Elution Buffer |
10 mL |
30 mL |
| RNase A |
175 μL |
625 μL |
| User Manual |
1 |
1 |
* Add ethanol to washing buffer before use
