EasyPrep™ EndoFree Plasmid Midiprep-II Kit provides a simple, fast and cost effective method to purify plasmid DNAs from bacterial culture, when amount of the plasmid DNA needed is about 500 µg. The kit is especially convenient to use when multiple plasmids need to be purified at the same time, as the use of traditional commercial kits is time-consuming. Key to the kit is our proprietary DNA binding systems that allow the high efficient DNA binding  while proteins and other impurities are removed by wash buffer. Nucleic acids are easily eluted with Elution Buffer. Our proprietary endotoxin removal buffer, Buffer RET, is designed to remove endotoxin by a single washing step without tedious phase partitioning steps. The purified plasmid DNA, with endotoxin level less than 0.1 EU per µg of DNA, is ready for transfection of endotoxin sensitive cell lines and microinjections. Kit's Content SKU PD04-21PD04-31 PD04-22PD04-32 Midiprep-II DNA Columns 10 25 ezFilter 10 (PD04-31) 25 (PD04-32) Buffer A1 60 ml 140 ml Buffer A2 60 ml 140 ml Buffer N3 30 ml 70 ml Buffer KB 60 ml 140 ml Buffer RET 100 ml 250 ml DNA Wash Buffer (5x)* 50 ml 2x54 ml Column Buffer 35 ml 90 ml EndoFree Elution Buffer  15 ml 30 ml RNase A 220 µl 520 µl Manual 1 1 * Add ethanol to washing buffer before use

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EasyPrep™ EndoFree Plasmid Midiprep-II Kit provides a simple, fast and cost effective method to purify plasmid DNAs from bacterial culture, when amount of the plasmid DNA needed is about 500 µg. The kit is especially convenient to use when multiple plasmids need to be purified at the same time, as the use of traditional commercial kits is time-consuming. Key to the kit is our proprietary DNA binding systems that allow the high efficient DNA binding  while proteins and other impurities are removed by wash buffer. Nucleic acids are easily eluted with Elution Buffer. Our proprietary endotoxin removal buffer, Buffer RET, is designed to remove endotoxin by a single washing step without tedious phase partitioning steps. The purified plasmid DNA, with endotoxin level less than 0.1 EU per µg of DNA, is ready for transfection of endotoxin sensitive cell lines and microinjections. Kit's Content SKU PD04-21PD04-31 PD04-22PD04-32 Midiprep-II DNA Columns 10 25 ezFilter 10 (PD04-31) 25 (PD04-32) Buffer A1 60 ml 140 ml Buffer A2 60 ml 140 ml Buffer N3 30 ml 70 ml Buffer KB 60 ml 140 ml Buffer RET 100 ml 250 ml DNA Wash Buffer (5x)* 50 ml 2x54 ml Column Buffer 35 ml 90 ml EndoFree Elution Buffer  15 ml 30 ml RNase A 220 µl 520 µl Manual 1 1 * Add ethanol to washing buffer before use

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EasyPrep™ EndoFree Plasmid Midiprep Kit provides a simple, fast and cost effective method to purify plasmid DNAs from bacterial culture, when amount of the plasmid DNA needed is about 200 µg. The kit is especially convenient to use when multiple plasmids need to be purified at the same time, as the use of traditional commercial kits is time-consuming. Key to the kit is our proprietary DNA binding systems that allow the high efficient DNA binding  while proteins and other impurities are removed by wash buffer. Nucleic acids are easily eluted with Elution Buffer. Our proprietary endotoxin removal buffer, Buffer RET, is designed to remove endotoxin by a single washing step without tedious phase partitioning steps. The purified plasmid DNA, with endotoxin level less than 0.1 EU per µg of DNA, is ready for transfection of endotoxin sensitive cell lines and microinjections. Kit's Contents SKU PD03-21PD03-31 PD03-22PD03-32 PD03-23PD03-33 Midiprep DNA Columns 10 25 50 EasyFilter 10 (PD03-31) 25 (PD03-32) 50 (PD03-33) Buffer A1 30 ml 75 ml 150 ml Buffer B1 30 ml 75 ml 150 ml Buffer N3 15 ml 30 ml 60 ml Buffer RET 30 ml 75 ml 150 ml DNA Wash Buffer (5x)* 25 ml 50 ml 2x50 ml EndoFree Elution Buffer 10 ml 20 ml 30 ml RNase A 110 µl 250 µl 500 ul Manual 1 1 1 * Add ethanol to washing buffer before use

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EasyPrep™ EndoFree Plasmid Midiprep Kit provides a simple, fast and cost effective method to purify plasmid DNAs from bacterial culture, when amount of the plasmid DNA needed is about 200 µg. The kit is especially convenient to use when multiple plasmids need to be purified at the same time, as the use of traditional commercial kits is time-consuming. Key to the kit is our proprietary DNA binding systems that allow the high efficient DNA binding  while proteins and other impurities are removed by wash buffer. Nucleic acids are easily eluted with Elution Buffer. Our proprietary endotoxin removal buffer, Buffer RET, is designed to remove endotoxin by a single washing step without tedious phase partitioning steps. The purified plasmid DNA, with endotoxin level less than 0.1 EU per µg of DNA, is ready for transfection of endotoxin sensitive cell lines and microinjections. Kit's Contents SKU PD03-21PD03-31 PD03-22PD03-32 PD03-23PD03-33 Midiprep DNA Columns 10 25 50 EasyFilter 10 (PD03-31) 25 (PD03-32) 50 (PD03-33) Buffer A1 30 ml 75 ml 150 ml Buffer B1 30 ml 75 ml 150 ml Buffer N3 15 ml 35 ml 70 ml Buffer RET 30 ml 75 ml 150 ml DNA Wash Buffer (5x)* 25 ml 50 ml 2x50 ml EndoFree Elution Buffer 10 ml 20 ml 30 ml RNase A 110 µl 250 µl 500 ul Manual 1 1 1 * Add ethanol to washing buffer before use

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EasyPrep™ EndoFree Plasmid Midiprep Kit provides a simple, fast and cost effective method to purify plasmid DNAs from bacterial culture, when amount of the plasmid DNA needed is about 200 µg. The kit is especially convenient to use when multiple plasmids need to be purified at the same time, as the use of traditional commercial kits is time-consuming. Key to the kit is our proprietary DNA binding systems that allow the high efficient DNA binding  while proteins and other impurities are removed by wash buffer. Nucleic acids are easily eluted with Elution Buffer. Our proprietary endotoxin removal buffer, Buffer RET, is designed to remove endotoxin by a single washing step without tedious phase partitioning steps. The purified plasmid DNA, with endotoxin level less than 0.1 EU per µg of DNA, is ready for transfection of endotoxin sensitive cell lines and microinjections. Kit's Contents SKU PD03-21PD03-31 PD03-22PD03-32 PD03-23PD03-33 Midiprep DNA Columns 10 25 50 EasyFilter 10 (PD03-31) 25 (PD03-32) 50 (PD03-33) Buffer A1 30 ml 75 ml 150 ml Buffer B1 30 ml 75 ml 150 ml Buffer N3 15 ml 35 ml 70 ml Buffer RET 30 ml 75 ml 150 ml DNA Wash Buffer (5x)* 25 ml 50 ml 2x50 ml EndoFree Elution Buffer 10 ml 20 ml 30 ml RNase A 110 µl 250 µl 500 ul Manual 1 1 1 * Add ethanol to washing buffer before use

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Key to the kit is our proprietary DNA binding systems that allow the high efficient reversible binding of DNA to the mini column while proteins and other impurities are removed by wash buffer. Nucleic acids are then eluted with sterile water or elution buffer. Plasmid isolated with traditional protocol normally contains high level of endotoxins (lipopolysaccharides or LPS). For transfection of endotoxin sensitive cell lines or microinjection, the endotoxins should be removed before the applications. The EasyPrep™ endofree system uses a specially formulated buffer that extracts the endotoxin from the plasmid DNA. Two rounds of extraction will reduce the endotoxin level to 0.1 EU (Endotoxin) per µg of plasmid DNA. The endofree plasmid miniprep kit provides an efficient endotoxin removal step into the traditional purification procedure to produce transfection grade plasmid DNA. This kit is designed for fast and efficient purification of plasmid DNA from 3 to 10 mL of E. coli culture. The mini column II has a DNA binding capacity of 80 µg. The purified endofree DNA is ready for downstream applications such as transfection of endotoxin-sensitive cell lines, primary cultured cells or microinjection. Kit's Contents SKU PD02-11 PD02-12 Preps 50 250 DNA Miniprep-II Columns 50 250 Buffer A1 25 mL 125 mL Buffer A2 25 mL 125 mL Buffer N3 5 mL 25 mL Buffer KB 30 mL 135 mL Buffer RET 50 mL 250 mL DNA Wash Buffer* 20 mL 3 x 24 mL EndoFree Elution Buffer 10 mL 30 mL RNase A 175 μL 625 μL User Manual 1 1 * Add ethanol to washing buffer before use  

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For 100 plasmid maxi preps (up to 2 ml/Plasmid prep).

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Eppendorf - Refurbished Pipettes Research Plus, 8-channel MuLtichannel Pipette Variable, 10 - 100 uL Product Description: The Eppendorf Research Plus Multichannel Pipette combines the spring-loaded tip cone, a secondary calibration option and improved volume display all in an ultra-light, fully autoclavable pipette. The spring-loaded tip cone saves your energy and time. No need for rocking, the spring guarantees a reproducible and safe tip fit. It provides increased user to user reproducibility for more uniform results among members of the lab. Eppendorf Research Plus Multichannel Pipette features and benefits: Air-displacement pipette for accurate pipetting of aqueous solutions. Feel the difference in weight and pipetting forces: the ultra-light mechanical pipette is designed according to the strict criteria of the Eppendorf PhysioCare Concept and limits the strain on your hand and arm. Spring-loaded tip cone for minimal tip attachment forces helps to reduce stress. The low tip ejection force can be as low as 3.6 N. Adjust your pipette in seconds for better accuracy when pipetting various difficult liquids like ethanol or even when pipetting at high altitudes. Return to factory adjustment without calibration. Autoclave the entire pipette or only the lower part according to your needs to ensure decontamination. Enjoy excellent flexibility and choice of variable volume 8 and 12 channel pipettes. Applications: Forward pipetting. Reverse pipetting. Removal of supernatants. Sample mixing. Phase extraction. Filling of plates, gels, and reaction vessels. Model Test Tip epT.I.P.S. Test Volume (μl) Systematic error (±%) Systematic error(±μl) Random error (±%) Random error(±μl) 10μl - 100μl yellow 8/12 channel 2μl - 200μl yellow 53 mm 10 3.0 0.3 2.0 0.2 50 1.0 0.5 0.8 0.4 100 0.8 0.8 0.3 0.3

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Eppendorf - Refurbished Pipettes Research Plus, 8-channel Multichannel Pipette Variable, 120 - 1200 uL Product Description: The Eppendorf Research Plus Multichannel Pipette combines the spring-loaded tip cone, a secondary calibration option and improved volume display all in an ultra-light, fully autoclavable pipette. The spring-loaded tip cone saves your energy and time. No need for rocking, the spring guarantees a reproducible and safe tip fit. It provides increased user to user reproducibility for more uniform results among members of the lab. Eppendorf Research Plus Multichannel Pipette features and benefits: Air-displacement pipette for accurate pipetting of aqueous solutions. Feel the difference in weight and pipetting forces: the ultra-light mechanical pipette is designed according to the strict criteria of the Eppendorf PhysioCare Concept and limits the strain on your hand and arm. Spring-loaded tip cone for minimal tip attachment forces helps to reduce stress. The low tip ejection force can be as low as 3.6 N. Adjust your pipette in seconds for better accuracy when pipetting various difficult liquids like ethanol or even when pipetting at high altitudes. Return to factory adjustment without calibration. Autoclave the entire pipette or only the lower part according to your needs to ensure decontamination. Enjoy excellent flexibility and choice of variable volume 8 and 12 channel pipettes. Applications: Forward pipetting. Reverse pipetting. Removal of supernatants. Sample mixing. Phase extraction. Filling of plates, gels, and reaction vessels. Model Test Tip epT.I.P.S. Test Volume (μl) Systematic error (±%) Systematic error(±μl) Random error (±%) Random error(±μl) 120μl - 1200μl dark green 8/12 channel 50μl - 1250μl dark green 103 mm 120  6.0 7.2 0.9 1.08 600 2.7 16.2 0.4 2.4 1200 1.2 14.4 0.3 3.6

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≥99.7% EtOH CAS No: 64-17-5 Formula: C2H6O or CH3CH2OH Molecular weight: 46.07g/mol Density: 0.7900g/mL Storage temperature: 5 to 30°C Boiling point: 78°C Melting point: -114°C Flash point: 14°C Meets ACS specifications Manufactured in ISO9001 certified facilities  Packaged under nitrogen 0.2um filtered Container material: HDPE Ship to US inland address only. This item is not for cross border or international shipping! Safety Data Sheet MSDS

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≥99.7% EtOH CAS No: 64-17-5 Formula: C2H6O or CH3CH2OH Molecular weight: 46.07g/mol Density: 0.7900g/mL Storage temperature: 5 to 30°C Boiling point: 78°C Melting point: -114°C Flash point: 14°C Meets ACS specifications Manufactured in ISO9001 certified facilities  Packaged under nitrogen 0.2um filtered Container material: HDPE Ship to US inland address only. This item is not for cross border or international shipping! Safety Data Sheet MSDS

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≥99.7% EtOH CAS No: 64-17-5 Formula: C2H6O or CH3CH2OH Molecular weight: 46.07g/mol Density: 0.7900g/mL Storage temperature: 5 to 30°C Boiling point: 78°C Melting point: -114°C Flash point: 14°C Meets ACS specifications Manufactured in ISO9001 certified facilities  Packaged under nitrogen 0.2um filtered Container material: HDPE Ship to US inland address only. This item is not for cross border or international shipping! Safety Data Sheet MSDS

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