A negative control siRNA should be included in a complete siRNA experiment. Our negative control siRNA and fluorescence-labeled negative control siRNA don’t have homology with any mammalian gene. The fluorescence-labeled negative control siRNA can be used for optimizing transfection condition and as a non-specific negative control. 1 OD=2.5-3 nmol Shipping cost for all siNRA orders is $40.

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A negative control siRNA should be included in a complete siRNA experiment. Our negative control siRNA and fluorescence-labeled negative control siRNA don’t have homology with any mammalian gene. The fluorescence-labeled negative control siRNA can be used for optimizing transfection condition and as a non-specific negative control. 1 OD=2.5-3 nmol Shipping cost for all siNRA orders is $30.

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A positive control siRNA is a known siRNA which can effectively silence a specific gene. It can be used in a parallel control experiment to ensure optimized experimental conditions and the effect of gene silencing. Available for Positive Control siRNA duplex: Lamin A/C MAPK1 p53 GFP274 β-actin Vimentin Luciferase GL2 GAPDH Cyclophilin B 1 OD=2.5-3 nmol Shipping cost for all siNRA orders is $30.

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A positive control siRNA is a known siRNA which can effectively silence a specific gene. It can be used in a parallel control experiment to ensure optimized experimental conditions and the effect of gene silencing. Available for Positive Control siRNA duplex: Lamin A/C MAPK1 p53 GFP274 β-actin Vimentin Luciferase GL2 GAPDH Cyclophilin B 1 OD=2.5-3 nmol Shipping cost for all siNRA orders is $30.

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A positive control siRNA is a known siRNA which can effectively silence a specific gene. It can be used in a parallel control experiment to ensure optimized experimental conditions and the effect of gene silencing. Available for Positive Control siRNA duplex: Lamin A/C MAPK1 p53 GFP274 β-actin Vimentin Luciferase GL2 GAPDH Cyclophilin B 1 OD=2.5-3 nmol Shipping cost for all siNRA orders is $30.

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A positive control siRNA is a known siRNA which can effectively silence a specific gene. It can be used in a parallel control experiment to ensure optimized experimental conditions and the effect of gene silencing. Available for Positive Control siRNA duplex: Lamin A/C MAPK1 p53 GFP274 β-actin Vimentin Luciferase GL2 GAPDH Cyclophilin B 1 OD=2.5-3 nmol Shipping cost for all siNRA orders is $30.

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The siRNA oligo can be labeled in the four different ends of double stranded by multiple markers. The labeled siRNA can be observed by flow cytometry, fluorescence microscopy, and laser scanning confocal microscope, which can determine whether the transfection is effective, and optimize transfection conditions. The labeled siRNA can also be used in siRNA intracellular localization and double labeling experiments (with labeled antibody) to track those siRNA transfected cells, then the reduction of target protein expression will be integrated with the transfection. Labeling of the anti-sense 5’ end will influence the gene silencing activity, so labeling of this site is not recommended. Modification of any of other three ends has no influence on silencing activity. We recommend to modifying the 5’ end of sense strand, which is the best recognized chemical labeling locus. Fluorescence-label includes 5'-FAM (green), 5'-Cy3 (red) and 3'-Cy3 (red). The labeled siRNA oligos are in double stranded form (duplex), and purified by HPLC. 1 OD=2.5-3 nmol Shipping cost for all siNRA orders is $30.  

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The siRNA oligo can be labeled in the four different ends of double stranded by multiple markers. The labeled siRNA can be observed by flow cytometry, fluorescence microscopy, and laser scanning confocal microscope, which can determine whether the transfection is effective, and optimize transfection conditions. The labeled siRNA can also be used in siRNA intracellular localization and double labeling experiments (with labeled antibody) to track those siRNA transfected cells, then the reduction of target protein expression will be integrated with the transfection. Labeling of the anti-sense 5’ end will influence the gene silencing activity, so labeling of this site is not recommended. Modification of any of other three ends has no influence on silencing activity. We recommend to modifying the 5’ end of sense strand, which is the best recognized chemical labeling locus. Fluorescence-label includes 5'-FAM (green), 5'-Cy3 (red) and 3'-Cy3 (red). The labeled siRNA oligos are in double stranded form (duplex), and purified by HPLC. 1 OD=2.5-3 nmol Shipping cost for all siNRA orders is $30.  

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The siRNA oligo can be labeled in the four different ends of double stranded by multiple markers. The labeled siRNA can be observed by flow cytometry, fluorescence microscopy, and laser scanning confocal microscope, which can determine whether the transfection is effective, and optimize transfection conditions. The labeled siRNA can also be used in siRNA intracellular localization and double labeling experiments (with labeled antibody) to track those siRNA transfected cells, then the reduction of target protein expression will be integrated with the transfection. Labeling of the anti-sense 5’ end will influence the gene silencing activity, so labeling of this site is not recommended. Modification of any of other three ends has no influence on silencing activity. We recommend to modifying the 5’ end of sense strand, which is the best recognized chemical labeling locus. Fluorescence-label includes 5'-FAM (green), 5'-Cy3 (red) and 3'-Cy3 (red). The labeled siRNA oligos are in double stranded form (duplex), and purified by HPLC. 1 OD=2.5-3 nmol Shipping cost for all siNRA orders is $30.  

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The siRNA oligo can be labeled in the four different ends of double stranded by multiple markers. The labeled siRNA can be observed by flow cytometry, fluorescence microscopy, and laser scanning confocal microscope, which can determine whether the transfection is effective, and optimize transfection conditions. The labeled siRNA can also be used in siRNA intracellular localization and double labeling experiments (with labeled antibody) to track those siRNA transfected cells, then the reduction of target protein expression will be integrated with the transfection. Labeling of the anti-sense 5’ end will influence the gene silencing activity, so labeling of this site is not recommended. Modification of any of other three ends has no influence on silencing activity. We recommend to modifying the 5’ end of sense strand, which is the best recognized chemical labeling locus. Fluorescence-label includes 5'-FAM (green), 5'-Cy3 (red) and 3'-Cy3 (red). The labeled siRNA oligos are in double stranded form (duplex), and purified by HPLC. 1 OD=2.5-3 nmol Shipping cost for all siNRA orders is $30.  

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EasyPrep™ Fungal gDNA Miniprep Kits allow rapid and reliable isolation of high-quality total cellular DNA from a wide variety of Fungal species and tissues. Up to 100mg of wet tissue (or up to 50 mg dry tissue) can be processed in less than 1 hour. The system combines the reversible nucleic acid-binding properties of Bioland’s matrix with the speed and versatility of spin column technology to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from fungal tissue lysates. Purified DNA is suitable for PCR, restriction digestion, and hybridization techniques. There are no organic extractions thus reducing plastic waste and hands-on time to allow multiple samples to be processed in parallel. Storage and Stability All components of the EasyPrep™ Fungal DNA Miniprep Kit are stable for at least 12 months from date of purchase when stored at 22°C-25°C. During shipment or storage in cool ambient conditions, precipitates may form in Buffer FG 3. It is possible to dissolve such deposits by warming the solution at 37°C. Kit's Contents SKU GD06-01 GD06-02 DNA Columns 50 250 Collection Tubes 100 500 Buffer FG1 40 mL 180 mL Buffer FG2 12mL 50 mL Buffer FG3 15mL 200 mL Buffer CFG1 30 mL 130 mL DNA Wash Buffer 12 mL 54 mL Elution Buffer 15 mL 50 mL RNase A 100 μL 500 μL Manual 1 1  

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EasyPrep™ Fungal gDNA Miniprep Kits allow rapid and reliable isolation of high-quality total cellular DNA from a wide variety of Fungal species and tissues. Up to 100mg of wet tissue (or up to 50 mg dry tissue) can be processed in less than 1 hour. The system combines the reversible nucleic acid-binding properties of Bioland’s matrix with the speed and versatility of spin column technology to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from fungal tissue lysates. Purified DNA is suitable for PCR, restriction digestion, and hybridization techniques. There are no organic extractions thus reducing plastic waste and hands-on time to allow multiple samples to be processed in parallel. Storage and Stability All components of the EasyPrep™ Fungal DNA Miniprep Kit are stable for at least 12 months from date of purchase when stored at 22°C-25°C. During shipment or storage in cool ambient conditions, precipitates may form in Buffer FG 3. It is possible to dissolve such deposits by warming the solution at 37°C. Kit's Contents SKU GD06-01 GD06-02 DNA Columns 50 250 Collection Tubes 100 500 Buffer FG1 40 mL 180 mL Buffer FG2 12mL 50 mL Buffer FG3 15mL 200 mL Buffer CFG1 30 mL 130 mL DNA Wash Buffer 12 mL 54 mL Elution Buffer 15 mL 50 mL RNase A 100 μL 500 μL Manual 1 1  

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