10xTris-Glycine SDS Running Buffer is formulated for separation of proteins in their denatured state on Tris-Glycine gels. Tris-Glycine gels provide reproducible separation of a wide range of proteins into well-resolved bands. Composition: 10xTGS: Tris 250 mM, Glycine 1.92 M, 1%SDS, pH 8.3, Western blot running buffer Application: Tris-glycine-SDS (TGS) running buffer is the most commonly used buffer for sodium dodecyl sulfate – polyacrylamide gel electrophoresis (SDS-PAGE) of proteins. TGS is usually used for both the anode buffer and the cathode buffer. Recommended running conditions is 150 volts for mini vertical gel electrophoresis units. Usage Recommendations To prepare 1X TGS buffer, add 100 ml of 10X TGS buffer to 900 ml of deionized water and mix well.  MSDS

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Application 20xTris-HEPES SDS running buffer is formulated for SDS-PAGE gel electrophoresis, such as for Thermo Scientific Precise and Pierce Precast Gels. Composition 1xTris-HEPES SDS Running Buffer: Tris 0.1 M, HEPES 0.1M, SDS 3mM, pH 8 Storage Room temperature or 4oC 

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Application 20xTris-HEPES SDS running buffer is formulated for SDS-PAGE gel electrophoresis, such as for Thermo Scientific Precise and Pierce Precast Gels. Composition 1xTris-HEPES SDS Running Buffer: Tris 0.1 M, HEPES 0.1M, SDS 3mM, pH 8 Storage Room temperature or 4°C

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Application 20xTris-HEPES SDS running buffer is formulated for SDS-PAGE gel electrophoresis, such as for Thermo Scientific Precise and Pierce Precast Gels. Composition 1xTris-HEPES SDS Running Buffer: Tris 0.1 M, HEPES 0.1M, SDS 3mM, pH 8 Storage Room temperature or 4°C 

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TRIS-buffered saline with Tween-20 (10x TBST) is used as a washing buffer for Western blot and other immunoassays, such as ELISA and dot blot assay. It acts as a pH stabilizer, which enable washing without disruption of antibody-antigen binding interactions. Tween 20 reduces nonspecific binding and protein: protein interactions. Each 10X TBST solution is ready to use upon dilution to the desired concentration.  Product Specification Appearance Clear, Colorless Form Liquid pH 7.4 ± 0.2 Composition (1x) 50mM Tris, 150mM NaCl, 0.05% Tween-20 Comment All components except Tween-20 are dissolved in ultrapure (Type I) water, followed by pH adjustment and filtered through 0.45 micron filter. Tween-20 is added and mixed before shipment. MSDS

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TRIS-buffered saline with Tween-20 (10x TBST) is used as a washing buffer for Western blot and other immunoassays, such as ELISA and dot blot assay. It acts as a pH stabilizer, which enable washing without disruption of antibody-antigen binding interactions. Tween 20 reduces nonspecific binding and protein: protein interactions. Each 10X TBST solution is ready to use upon dilution to the desired concentration.  Product Specification Appearance Clear, Colorless Form Liquid pH 7.6 ± 0.2 Composition (1x) 25mM Tris, 137mM NaCl, 2.7mM KCl, 0.05% Tween-20 Comment All components except Tween-20 are dissolved in ultrapure (Type I) water, followed by pH adjustment and filtered through 0.45 micron filter. Tween-20 is added and mixed before shipment. MSDS

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TRIS-buffered saline with Tween-20 (10x TBST) is used as a washing buffer for Western blot and other immunoassays, such as ELISA and dot blot assay. It acts as a pH stabilizer, which enable washing without disruption of antibody-antigen binding interactions. Tween 20 reduces nonspecific binding and protein: protein interactions. Each 10X TBST solution is ready to use upon dilution to the desired concentration.  Product Specification Appearance Clear, Colorless Form Liquid pH 7.4 ± 0.2 Composition (1x) 50mM Tris, 150mM NaCl, 0.05% Tween-20 Comment All components except Tween-20 are dissolved in ultrapure (Type I) water, followed by pH adjustment and filtered through 0.45 micron filter. Tween-20 is added and mixed before shipment. MSDS

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TRIS-buffered saline with Tween-20 (10x TBST) is used as a washing buffer for Western blot and other immunoassays, such as ELISA and dot blot assay. It acts as a pH stabilizer, which enable washing without disruption of antibody-antigen binding interactions. Tween 20 reduces nonspecific binding and protein: protein interactions. Each 10X TBST solution is ready to use upon dilution to the desired concentration.  Product Specification Appearance Clear, Colorless Form Liquid pH 7.6 ± 0.2 Composition (1x) 25mM Tris, 137mM NaCl, 2.7mM KCl, 0.05% Tween-20 Comment All components except Tween-20 are dissolved in ultrapure (Type I) water, followed by pH adjustment and filtered through 0.45 micron filter. Tween-20 is added and mixed before shipment. MSDS

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TRIS-buffered saline with Tween-20 (10x TBST) is used as a washing buffer for Western blot and other immunoassays, such as ELISA and dot blot assay. It acts as a pH stabilizer, which enable washing without disruption of antibody-antigen binding interactions. Tween 20 reduces nonspecific binding and protein: protein interactions. Each 10X TBST solution is ready to use upon dilution to the desired concentration.  Product Specification Appearance Clear, Colorless Form Liquid pH 7.4 ± 0.2 Composition (1x) 50mM Tris, 150mM NaCl, 0.05% Tween-20 Comment All components except Tween-20 are dissolved in ultrapure (Type I) water, followed by pH adjustment and filtered through 0.45 micron filter. Tween-20 is added and mixed before shipment. MSDS

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TRIS-buffered saline with Tween-20 (10x TBST) is used as a washing buffer for Western blot and other immunoassays, such as ELISA and dot blot assay. It acts as a pH stabilizer, which enable washing without disruption of antibody-antigen binding interactions. Tween 20 reduces nonspecific binding and protein: protein interactions. Each 10X TBST solution is ready to use upon dilution to the desired concentration.  Product Specification Appearance Clear, Colorless Form Liquid pH 7.6 ± 0.2 Composition (1x) 25mM Tris, 137mM NaCl, 2.7mM KCl, 0.05% Tween-20 Comment All components except Tween-20 are dissolved in ultrapure (Type I) water, followed by pH adjustment and filtered through 0.45 micron filter. Tween-20 is added and mixed before shipment. MSDS

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1xTG: Tris 25 mM, Glycine 192 mM, pH 8.3, with 20% methanol. Western blot transfer buffer Application: For Western blotting and gel electrophoresis. Tris-glycine buffer is used to make a Tris-glycine-methanol transfer buffer, which is the most common protein transfer buffer for wet blot transfers. The methanol prevents the gel from swelling during the transfer and enhances the protein binding to membranes. Usage Recommendations: To prepare 1X TG transfer buffer:  add 100 ml of 10X TG buffer to 700 ml of deionized water, then add 200 ml methanol and mix well.  Use fresh 1X TG transfer buffer. A sufficient amount of transfer buffer should be made to cover the electrode wires in the wet blot transfer unit and to soak the gel, membrane and blotting paper. MSDS

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1xTG: Tris 25 mM, Glycine 192 mM, pH 8.3, with 20% methanol. Western blot transfer buffer Application: For Western blotting and gel electrophoresis. Tris-glycine buffer is used to make a Tris-glycine-methanol transfer buffer, which is the most common protein transfer buffer for wet blot transfers. The methanol prevents the gel from swelling during the transfer and enhances the protein binding to membranes. Usage Recommendations: To prepare 1X TG transfer buffer:  add 100 ml of 10X TG buffer to 700 ml of deionized water, then add 200 ml methanol and mix well.  Use fresh 1X TG transfer buffer. A sufficient amount of transfer buffer should be made to cover the electrode wires in the wet blot transfer unit and to soak the gel, membrane and blotting paper. MSDS

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