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10xTBST Buffer (1 L)

    Description

    TRIS-buffered saline with Tween-20 (10x TBST) is used as a washing buffer for Western blot and other immunoassays, such as ELISA and dot blot assay. It acts as a pH stabilizer, which enable washing without disruption of antibody-antigen binding interactions. Tween 20 reduces nonspecific binding and protein: protein interactions. Each 10X TBST solution is ready to use upon dilution to the desired concentration. 

    Product Specification

    Appearance Clear, Colorless
    Form Liquid
    pH 7.6 ± 0.2
    Composition (1x) 25mM Tris, 137mM NaCl, 2.7mM KCl, 0.05% Tween-20
    Comment All components except Tween-20 are dissolved in ultrapure (Type I) water, followed by pH adjustment and filtered through 0.45 micron filter. Tween-20 is added and mixed before shipment.

    MSDS

    Product form

    SKU: TBST03-1L

    10xTBST Buffer (1 L)

      TRIS-buffered saline with Tween-20 (10x TBST) is used as a washing buffer for Western blot and other immunoassays, such as... Read more

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          Description

          TRIS-buffered saline with Tween-20 (10x TBST) is used as a washing buffer for Western blot and other immunoassays, such as ELISA and dot blot assay. It acts as a pH stabilizer, which enable washing without disruption of antibody-antigen binding interactions. Tween 20 reduces nonspecific binding and protein: protein interactions. Each 10X TBST solution is ready to use upon dilution to the desired concentration. 

          Product Specification

          Appearance Clear, Colorless
          Form Liquid
          pH 7.6 ± 0.2
          Composition (1x) 25mM Tris, 137mM NaCl, 2.7mM KCl, 0.05% Tween-20
          Comment All components except Tween-20 are dissolved in ultrapure (Type I) water, followed by pH adjustment and filtered through 0.45 micron filter. Tween-20 is added and mixed before shipment.

          MSDS

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