TRIS-buffered saline with Tween-20 (10x TBST) is used as a washing buffer for Western blot and other immunoassays, such as ELISA and dot blot assay. It acts as a pH stabilizer, which enable washing without disruption of antibody-antigen binding interactions. Tween 20 reduces nonspecific binding and protein: protein interactions. Each 10X TBST solution is ready to use upon dilution to the desired concentration.
Product Specification
| Appearance |
Clear, Colorless |
| Form |
Liquid |
| pH |
7.6 ± 0.2 |
| Composition (1x) |
25mM Tris, 137mM NaCl, 2.7mM KCl, 0.05% Tween-20 |
| Comment |
All components except Tween-20 are dissolved in ultrapure (Type I) water, followed by pH adjustment and filtered through 0.45 micron filter. Tween-20 is added and mixed before shipment. |
MSDS
