10xTBST Buffer (10 L)

    Description

    TRIS-buffered saline with Tween-20 (10x TBST) is used as a washing buffer for Western blot and other immunoassays, such as ELISA and dot blot assay. It acts as a pH stabilizer, which enable washing without disruption of antibody-antigen binding interactions. Tween 20 reduces nonspecific binding and protein: protein interactions. Each 10X TBST solution is ready to use upon dilution to the desired concentration. 

    Product Specification

    Appearance Clear, Colorless
    Form Liquid
    pH 7.4 ± 0.2
    Composition (1x) 50mM Tris, 150mM NaCl, 0.05% Tween-20
    Comment All components except Tween-20 are dissolved in ultrapure (Type I) water, followed by pH adjustment and filtered through 0.45 micron filter. Tween-20 is added and mixed before shipment.
    MSDS

    Product form

    SKU: TBST01-10L

    10xTBST Buffer (10 L)

      TRIS-buffered saline with Tween-20 (10x TBST) is used as a washing buffer for Western blot and other immunoassays, such as... Read more

      $141.20

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          MSDS

        Description

        TRIS-buffered saline with Tween-20 (10x TBST) is used as a washing buffer for Western blot and other immunoassays, such as ELISA and dot blot assay. It acts as a pH stabilizer, which enable washing without disruption of antibody-antigen binding interactions. Tween 20 reduces nonspecific binding and protein: protein interactions. Each 10X TBST solution is ready to use upon dilution to the desired concentration. 

        Product Specification

        Appearance Clear, Colorless
        Form Liquid
        pH 7.4 ± 0.2
        Composition (1x) 50mM Tris, 150mM NaCl, 0.05% Tween-20
        Comment All components except Tween-20 are dissolved in ultrapure (Type I) water, followed by pH adjustment and filtered through 0.45 micron filter. Tween-20 is added and mixed before shipment.
        MSDS

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