TRIS-buffered saline (10x TBS) is used as a washing buffer for Western blot and other alkaline phosphatase and peroxidase conjugates assay, such as ELISA and dot blot assay. It acts as a pH stabilizer, which enable washing without disruption of antibody-antigen binding interactions. Each 10X TBS solution is ready to use upon dilution to the desired concentration.  Product Specification Appearance Clear, Colorless Form Liquid pH 7.4 ± 0.2 Composition (1x) 50mM Tris, 150mM NaCl Comment All components are dissolved in ultrapure (Type I) water, followed by pH adjustment and filtered through 0.45 micron filter. MSDS

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TRIS-buffered saline (10x TBS) is used as a washing buffer for Western blot and other alkaline phosphatase and peroxidase conjugates assay, such as ELISA and dot blot assay. It acts as a pH stabilizer, which enable washing without disruption of antibody-antigen binding interactions. Each 10X TBS solution is ready to use upon dilution to the desired concentration.  Product Specification Appearance Clear, Colorless Form Liquid pH 7.6 ± 0.2 Composition (1x) 25mM Tris, 137mM NaCl, 2.7mM KCl Comment All components are dissolved in ultrapure (Type I) water, followed by pH adjustment and filtered through 0.45 micron filter.  MSDS

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TRIS-buffered saline (10x TBS) is used as a washing buffer for Western blot and other alkaline phosphatase and peroxidase conjugates assay, such as ELISA and dot blot assay. It acts as a pH stabilizer, which enable washing without disruption of antibody-antigen binding interactions. Each 10X TBS solution is ready to use upon dilution to the desired concentration.  Product Specification Appearance Clear, Colorless Form Liquid pH 7.4 ± 0.2 Composition (1x) 50mM Tris, 150mM NaCl Comment All components are dissolved in ultrapure (Type I) water, followed by pH adjustment and filtered through 0.45 micron filter. MSDS

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TRIS-buffered saline (10x TBS) is used as a washing buffer for Western blot and other alkaline phosphatase and peroxidase conjugates assay, such as ELISA and dot blot assay. It acts as a pH stabilizer, which enable washing without disruption of antibody-antigen binding interactions. Each 10X TBS solution is ready to use upon dilution to the desired concentration.  Product Specification Appearance Clear, Colorless Form Liquid pH 7.6 ± 0.2 Composition (1x) 25mM Tris, 137mM NaCl, 2.7mM KCl Comment All components are dissolved in ultrapure (Type I) water, followed by pH adjustment and filtered through 0.45 micron filter.  MSDS

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10x Tricine SDS Running Buffer is formulated for separation of proteins in their denatured state on tricine gels. Tricine gels are specifically designed for the resolution of low molecular weight proteins. In this buffer system, tricine substitutes glycine in the running buffer, resulting in more efficient stacking and destacking of low molecular weight proteins and higher resolution of smaller peptides.Recommended buffers: Use with tricine sample buffer to obtain optimal separation with tricine SDS running buffer. Composition: 1x buffer contains Tris base (100 mM), tricine (100 mM), SDS (0.1%), pH 8.3 Storage: 4oC to room temperature for 1 year

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10x Tricine SDS Running Buffer is formulated for separation of proteins in their denatured state on tricine gels. Tricine gels are specifically designed for the resolution of low molecular weight proteins. In this buffer system, tricine substitutes glycine in the running buffer, resulting in more efficient stacking and destacking of low molecular weight proteins and higher resolution of smaller peptides.Recommended buffers: Use with tricine sample buffer to obtain optimal separation with tricine SDS running buffer. Composition: 1x buffer contains Tris base (100 mM), tricine (100 mM), SDS (0.1%), pH 8.3 Storage: 4°C to room temperature for 1 year

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10x Tricine SDS Running Buffer is formulated for separation of proteins in their denatured state on tricine gels. Tricine gels are specifically designed for the resolution of low molecular weight proteins. In this buffer system, tricine substitutes glycine in the running buffer, resulting in more efficient stacking and destacking of low molecular weight proteins and higher resolution of smaller peptides.Recommended buffers: Use with tricine sample buffer to obtain optimal separation with tricine SDS running buffer. Composition: 1x buffer contains Tris base (100 mM), tricine (100 mM), SDS (0.1%), pH 8.3 Storage: 4°C to room temperature for 1 year

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10xTris-Glycine Native Running Buffer is designed for protein gel electrophoresis under native running conditions with Tris-Glycine gels or Tris-Acetate gels. Tris-Glycine gels do not contain SDS and can be used to accurately separate both native and denatured proteins, depending upon the sample and running buffers used. To separate denatured proteins on Tris-Glycine gels, use Tris-Glycine SDS Sample Buffer and Tris-Glycine SDS Running Buffer. To separate native proteins use Tris-Glycine Native Sample Buffer and Tris-Glycine Native Running Buffer. Composition: 10xTGS: Tris 250 mM, Glycine 1.92 M, pH 8.3 Usage Recommendations To prepare 1X TG buffer, add 100 ml of 10X TG buffer to 900 ml of deionized water and mix well. 

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10xTris-Glycine Native Running Buffer is designed for protein gel electrophoresis under native running conditions with Tris-Glycine gels or Tris-Acetate gels. Tris-Glycine gels do not contain SDS and can be used to accurately separate both native and denatured proteins, depending upon the sample and running buffers used. To separate denatured proteins on Tris-Glycine gels, use Tris-Glycine SDS Sample Buffer and Tris-Glycine SDS Running Buffer. To separate native proteins use Tris-Glycine Native Sample Buffer and Tris-Glycine Native Running Buffer. Composition: 10xTGS: Tris 250 mM, Glycine 1.92 M, pH 8.3 Usage Recommendations To prepare 1X TG buffer, add 100 ml of 10X TG buffer to 900 ml of deionized water and mix well. 

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10xTris-Glycine Native Running Buffer is designed for protein gel electrophoresis under native running conditions with Tris-Glycine gels or Tris-Acetate gels. Tris-Glycine gels do not contain SDS and can be used to accurately separate both native and denatured proteins, depending upon the sample and running buffers used. To separate denatured proteins on Tris-Glycine gels, use Tris-Glycine SDS Sample Buffer and Tris-Glycine SDS Running Buffer. To separate native proteins use Tris-Glycine Native Sample Buffer and Tris-Glycine Native Running Buffer. Composition: 10xTGS: Tris 250 mM, Glycine 1.92 M, pH 8.3 Usage Recommendations To prepare 1X TG buffer, add 100 ml of 10X TG buffer to 900 ml of deionized water and mix well. 

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10xTris-Glycine SDS Running Buffer is formulated for separation of proteins in their denatured state on Tris-Glycine gels. Tris-Glycine gels provide reproducible separation of a wide range of proteins into well-resolved bands. Composition: 10xTGS: Tris 250 mM, Glycine 1.92 M, 1%SDS, pH 8.3, Western blot running buffer Application: Tris-glycine-SDS (TGS) running buffer is the most commonly used buffer for sodium dodecyl sulfate – polyacrylamide gel electrophoresis (SDS-PAGE) of proteins. TGS is usually used for both the anode buffer and the cathode buffer. Recommended running conditions is 150 volts for mini vertical gel electrophoresis units. Usage Recommendations To prepare 1X TGS buffer, add 100 ml of 10X TGS buffer to 900 ml of deionized water and mix well.  MSDS

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10xTris-Glycine SDS Running Buffer is formulated for separation of proteins in their denatured state on Tris-Glycine gels. Tris-Glycine gels provide reproducible separation of a wide range of proteins into well-resolved bands. Composition: 10xTGS: Tris 250 mM, Glycine 1.92 M, 1%SDS, pH 8.3, Western blot running buffer Application: Tris-glycine-SDS (TGS) running buffer is the most commonly used buffer for sodium dodecyl sulfate – polyacrylamide gel electrophoresis (SDS-PAGE) of proteins. TGS is usually used for both the anode buffer and the cathode buffer. Recommended running conditions is 150 volts for mini vertical gel electrophoresis units. Usage Recommendations To prepare 1X TGS buffer, add 100 ml of 10X TGS buffer to 900 ml of deionized water and mix well.  MSDS

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