Synonyms: alpha-D-Glucopyranoside , Sucrose , D-(+)-Saccharose , D(+)-Saccharose , beta-D-Fructofuranosyl alpha-D-glucopyranoside , Sugar Formula: C12H22O11 MW: 342.3 g/mol Storage Temperature: Ambient CAS Number: 57-50-1 EINECS: 200-334-9 Categories Specification Result Appearance White crystalline powder Conform Specific optical rotation 66.2° - 66.7° Conform Appearance of solution Comply with the standard Conform Water Insoluble ≤0.002% 0.001% Loss on drying ≤0.03% 0.02% Residue on ignition ≤0.01% 0.007% Acidity ≤0.08% 0.03% Chloride ≤0.0005% 0.0003% Sulphates ≤0.002% 0.001% Iron ≤0.00001% 0.00002% Heavy metals ≤0.0005% 0.00005% Reducing sugar Comply with the standard Conform Safety Data Sheet MSDS AR Grade- The standard Mallinckrodt grade of analytical reagents; suitable for laboratory and general use. Analytical Research Grade chemicals are a very high grade of chemical suitable for the specialist analytical testing carried out in laboratories where a high degree of accuracy is required.

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Synonyms: alpha-D-Glucopyranoside , Sucrose , D-(+)-Saccharose , D(+)-Saccharose , beta-D-Fructofuranosyl alpha-D-glucopyranoside , Sugar Formula: C12H22O11 MW: 342.3 g/mol Storage Temperature: Ambient CAS Number: 57-50-1 EINECS: 200-334-9 Categories Specification Result Appearance White crystalline powder Conform Specific optical rotation 66.2° - 66.7° Conform Appearance of solution Comply with the standard Conform Water Insoluble ≤0.002% 0.001% Loss on drying ≤0.03% 0.02% Residue on ignition ≤0.01% 0.007% Acidity ≤0.08% 0.03% Chloride ≤0.0005% 0.0003% Sulphates ≤0.002% 0.001% Iron ≤0.00001% 0.00002% Heavy metals ≤0.0005% 0.00005% Reducing sugar Comply with the standard Conform Safety Data Sheet MSDS AR Grade- The standard Mallinckrodt grade of analytical reagents; suitable for laboratory and general use. Analytical Research Grade chemicals are a very high grade of chemical suitable for the specialist analytical testing carried out in laboratories where a high degree of accuracy is required.

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SuperHiFi™ High Fidelity DNA Polymerase Features Exceptionally high fidelity – Over 50-fold better fidelity than Taq DNA Polymerase, eqivalent to Phusion polymerase Extremely efficient – With extension speed of 3-4kb/min, significantly shorter extension time  Robust performance – Amplification of long templates (up to15 kb) PCR products have blunt end  

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Molecular cloning assisted by vectors is the most popular and common method to obtain genes of interest. Yeastern Biotech’s T&A™ Cloning Kit offers a quick, reliable and efficient method for cloning a variety of DNA sequences. The T&A™ Cloning Kit contains the T&A™ Cloning Vector and all the reagents needed for ligation. It is a convenient pack for cloning PCR product generated using thermostable DNA polymerases, such as YEAtaq DNA polymerase, which add a single terminal 3′-dA nucleotide overhang. After ligation, the mixture can be used directly for transformation into competent cells (ECOS™) or be purified first to achieve higher transformation efficiency. Features Fast ligation, completed in only 5 minutes More accurate results Accept a wide range of inserts with different sizes Two types of ligation buffers provided for your convenience Allow blue/white screening Contain ampicillin marker for antibiotic selection Include M13 primer sites for convenient sequencing Applications Cloning of terminal 3’-dA nucleotides overhang PCR products up to 5 kb Quality Control DNA concentration of T&A™ Cloning Vector is 25 ng/μl The absorbance ratio (A260/A280) is between 1.6~2.0 The size of T&A™ Cloning Vector is about 2.7 kb The colony number of background control is less than 50 when the transformation efficiency of competent cells is 1 × 108cfu/μg DNA The colony number ratio of self-ligation control to positive control is less than 15% The colony number of the positive control is more than 500 when the transformation efficiency of competent cells is 5 × 108cfu/μg DNA The ligation correctness with the control insert into T&A™ Cloning Vector is more than 87.5% Store At T&A™ Cloning Vector 40 μl (25 ng/ μl) Control Insert DNA 10 μl (10 ng/ μl) yT4 DNA Ligase 20 μl (2U/ μl) 10X Ligation Buffer A 50 μl 10X Ligation Buffer B 50 μl Forward Primer (M13-F) 50 μl (10 μM) Reverse Primer (M13-R) 50 μl (10 μM)   ***** Warning ! This is cold item, product requires to select Overnight shipping! *****

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Description This product is expressed by Escherichia coli, and the source of the expressed gene is T4 bacteriophage. Due to the simultaneous activity of 5'→3' DNA polymerase and 3'→5' DNA exonuclease, T4 DNA polymerase can be used to flatten the 5' protruding end or flatten the 3' protruding end. It can also be used for labeling DNA probe synthesis through displacement reactions, analyzing the starting point of mRNA transcription through primer elongation, synthesizing the second strand during site-specific mutagenesis, and cloning PCR products that do not rely on linkage reactions.The 3'→5' DNA exonuclease activity of this T4 DNA polymerase is about 100-1000 times higher than Klenow Fragment, and it has higher activity for single stranded DNA than double stranded DNA. This enzyme does not contain exonuclease activity of 5'→3' DNA, and can be inactivated by heating at 70°C for 10 minutes. Metal ion chelating agents can inhibit its activity. Unit definition The amount of enzyme required to incorporate 10 nmol of whole nucleotides into acid insoluble precipitate is defined as 1 active unit (U) within 30 minutes, using thermally denatured calf thymus DNA as a template/primer, at 37°C and pH 8.8. Quality control 2U of this enzyme reacted with 1μg of Closed Circular (RFI) pBR322 DNA at 37℃ for 16 hours, and the electrophoresis bands of the DNA remained unchanged. Shipping and Storage -20°C Components SKU TDP01-01 TDP01-02 T4 DNA Polymerase (3U/μl) 50 μl 250 μl 10×T4 DNA Polymerase Reaction Buffer 1 ml 4x1 ml

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T4 Polynucleotide Kinase, abbreviated as T4 PNK, is expressed in Escherichia coli. The gene is derived from T4 bacteriophage and can catalyze the phosphorylation of ATP γ- The 5'-hydroxy terminus and 3'-monophosphate nucleosides of the nucleotide chain (double stranded or single stranded DNA or RNA). T4 PNK also possesses 3'-phosphatase activity, which hydrolyze the 3'-phosphate group from the 3'-phosphate terminus of the oligonucleotide, deoxygenated 3'-monophosphate nucleoside, and deoxygenated 3'-diphosphate nucleoside. The T4 PNK can be used for 5' end labeling or phosphorylation of oligonucleotides, DNA, or RNA; the phosphorylation of 5' single nucleotide or removal of the 3' terminal phosphate group. Heating this product at 75°C for 10 minutes or adding EDTA can inactivate it. Ion chelating agents, phosphates, ammonium ions, KCl ≥50 mM, and NaCl can significantly inhibit its activity. Unit Definition At 37°C, within 30 minutes, the amount of enzyme required for the transfer of 1 nmol of γ- Phosphate group on ATP to the end of 5′-OH on DNA is defined as 1 active unit. Quality Control After multiple column purification, the purity of SDS-PAGE was more than 99%; No contamination of endonuclease, exonuclease, phosphatase and RNase activities was detected. Precautions Because the ammonium salt can strongly inhibit the activity of T4 polynucleotide kinase, the DNA obtained from ammonium salt precipitation cannot be used for the labeling reaction of T4 polynucleotide kinase. PEG can promote the rate and efficiency of phosphorylation reaction, and PEG should be added to the exchange reaction system. When using, the enzyme should be stored in an ice box or on an ice bath, and should be stored at -20°C immediately after use.

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Tank Sanitization Module RAPRC0127 can effectively keep microbial growth at a lower level inside the tank by UV light. Purification Technologies:  UV Light Suitable for: 30/60/100 L Genie tank

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Tank UV Lamp for Genie, 254nm RAUV357A7 can effectively keep microbial growth at a lower level. Purification Technologies:  Degerming for killing microorganisms Suitable for: 30/60/100 L Genie tank 100/350 L Super-Genie tank

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Tank Vent Filter with CO2 scavenger RATANKVN7 is designed to prevent airborne particles, CO2, organic and bacteria from entering the PE water tank. It contains a scavenger to remove CO2. Purification Technologies:  Activated Carbon Membrane Filtration Chemistry Adsorption Technical Specifications:  Height 393.8mm Diameter φ90mm Suitable for: 30 / 60 / 100 L Genie tank

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5 U/µl (1µl contains 5 units Taq DNA Polymerase) Amplify DNA target up to 6 kb Elongation velocity is 1kb/min Lacks of 3' to 5' exoneclease activity Makes 3'-dA overhangs PCR product, good for TA cloning 10x Reaction Buffer is included 

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A novel recombinant reverse transcriptase that exhibits much higher efficiency in the first-strand cDNA synthesis from RNA templates with secondary structures and high GC content. PowerScriptTM Plus Reverse Transcriptase is engineered to perform under high temperatures (50°C - 60°C), facilitating the elimination of secondary structures associated with GC-rich RNA templates. Due to this unique feature, ThermoScriptTM Plus can synthesize full-length cDNA libraries from RNA templates up to 12 kb in length.  Features  Lack RNase H activity: higher yield of cDNA.  Thermal stable: the optimum reaction temperature is 50°C, the highest is 60°C. Can overcome the template RNA secondary structure, and finish the reverse transcriptase experiment smoothly.  Wide temperature range: can reverse transcript from 37-60°C, with more than 80% of the highest activity at 42°C-60°C. customer can choose the reaction temperature freely.  Strong amplification activity: Gene mutation enhanced the binding capacity of the enzyme and RNA. Increased the amplification speed, can obtain the quality cDNA, suitable for cDNA library construction.  Applications Synthesizing cDNA from a ssRNA Constructing cDNA library Source Recombination of E. coli containing Moloney murine leukemia virus reverse transcriptase gene from clone of Moloney murine. Unit definition Using Poly (A) as the template and oligo (dT) as the primer, the enzyme required to catalyze the incorporation of 1 nmol of dTTP within 10 minutes at 37 oC is defined as one active unit (U). Concentration: 200 U/ul Size: 25 ul Form: Enzyme supplied with 5X RT buffer  Storage: Store all components at -20°C

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The EasyPrepTM DNA/RNA Kit provides a simple and rapid method for simultaneously isolating genomic DNA and total RNA from a biological samples, which includes cultured cells, tissues, whole blood, plasma, serum, and body fluids. This co-purification system saves time on sample handling, avoids the sample variation and saves precious samples, which is essential when the amount of available samples are limited Instead of using proteinase K to digest the biological materials, the EasyPrepTM DNA/RNA Kit applies the lysis buffer directly to the cells or tissues. After homogenization, the lysates are transferred to DNA columns. Thus the time-consuming steps of material preparation are eliminated. The DNA-binding-specific matrix in the DNA column allows the high efficient binding of DNA, while RNA, proteins and other cellular components are passed through the DNA columns and are collected. Genomic DNA are then purified from the DNA columns, while total RNA are purified from the collected eluate through RNA binding columns. Purified DNA can be directly used for downstream applications such as PCR, Southern blotting, sequencing and restriction enzyme digestion, while purified total RNA can be used for RT-PCR, Northern blotting or microarray analysis.  Kit's Contents SKU DR01-01 DR01-02 Storage Preps 50 250 - DNA columns 50 250 RT RNA columns 50 250 RT GD Lysis Buffer 60 ml 260 ml RT DNA Wash Buffer 12 ml 50 ml RT DNA Elution Buffer 10 ml 25 ml RT RNA Wash Buffer 12 ml 50 ml RT DEPC H2O 10 ml 25 ml RT Collection tubes 100 500 RT Manual 1 1 -  

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