The EasyPrepTM DNA/RNA Kit provides a simple and rapid method for simultaneously isolating genomic DNA and total RNA from a biological samples, which includes cultured cells, tissues, whole blood, plasma, serum, and body fluids. This co-purification system saves time on sample handling, avoids the sample variation and saves precious samples, which is essential when the amount of available samples are limited
Instead of using proteinase K to digest the biological materials, the EasyPrepTM DNA/RNA Kit applies the lysis buffer directly to the cells or tissues. After homogenization, the lysates are transferred to DNA columns. Thus the time-consuming steps of material preparation are eliminated. The DNA-binding-specific matrix in the DNA column allows the high efficient binding of DNA, while RNA, proteins and other cellular components are passed through the DNA columns and are collected. Genomic DNA are then purified from the DNA columns, while total RNA are purified from the collected eluate through RNA binding columns. Purified DNA can be directly used for downstream applications such as PCR, Southern blotting, sequencing and restriction enzyme digestion, while purified total RNA can be used for RT-PCR, Northern blotting or microarray analysis.
Kit's Contents
| SKU |
DRP01-01 |
DRP01-02 |
Storage |
Preps
|
50 |
250 |
- |
| DNA columns |
50 |
250 |
RT |
| RNA columns |
50 |
250 |
RT |
| GD Lysis Buffer |
60 ml |
260 ml |
RT |
| DNA Wash Buffer |
12 ml |
50 ml |
RT |
| DNA Elution Buffer |
10 ml |
25 ml |
RT |
| RNA Wash Buffer |
12 ml |
50 ml |
RT |
| DEPC H2O |
10 ml |
25 ml |
RT |
| Collection tubes |
100 |
500 |
RT |
| Protein Isolation Buffer |
40 ml |
200 ml |
RT |
| Manual |
1 |
1 |
- |
