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Yeast Genomic DNA Miniprep Kit (50 Preps)

    Description

    The EasyPrepTM Yeast gDNA Kit allows rapid and reliable isolation of high-quality total cellular DNA from a wide variety of yeast species. Up to 3 mL of log-phase culture (OD600 of 1.0 in YPD medium) can be processed. The system combines the reversible nucleic acid-binding properties of DNA-binding matrix with the speed and versatility of spin column technology to yield approximately 15-30 μg of DNA with an A260/A280 ratio of 1.7-1.9. The kit will isolate all cellular DNA, including plasmid DNA. Purified DNA is suitable for PCR, restriction digestion, and hybridization techniques. There are no organic extractions thus reducing plastic waste and hands-on time to allow multiple samples to be processed in parallel.

    Storage and Stability

    All components of the EasyPrepTM Yeast gDNA Kit, except the Proteinase K, RNase A and Lyticase can be stored at 22 -25 °C and are guaranteed for at least 24 months from the dated of purchase. Once reconstituted in water, Proteinase K and Lyticase must be stored at –20°C. Store RNase A at 4°C. Under cool ambient conditions, a precipitate may form in the Buffer YBL/YTL. In case of such an event, heat the bottle at 37°C to dissolve. Store Buffer YTL/ YBL at room temperature.

    Kit's Contents

    SKU GD05-01 GD05-02
    DNA Mini Columns
    50 250
    2 mL Collection Tubes 100 500
    Buffer YTL 20 mL 100 mL
    Buffer YBL 20 mL 100 mL
    Buffer KB 28 mL 135 mL
    DNA Wash Buffer 30 mL 3 x 36 mL
    Glass Beads 3 g 15 g
    Elution Buffer 15 mL 70 mL
    Buffer SE 30 mL 135 mL
    PK storage Buffer 5 mL 10 mL
    Lyticase (Units) 150 750
    Proteinase K 30 mg 150 mg
    RNase A 100 μL 500 μL
    Manual 1 1

     

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    SKU: GD05-01

    Yeast Genomic DNA Miniprep Kit (50 Preps)

      The EasyPrepTM Yeast gDNA Kit allows rapid and reliable isolation of high-quality total cellular DNA from a wide variety of... Read more

      $85.00 Excl. VAT

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          Description

          The EasyPrepTM Yeast gDNA Kit allows rapid and reliable isolation of high-quality total cellular DNA from a wide variety of yeast species. Up to 3 mL of log-phase culture (OD600 of 1.0 in YPD medium) can be processed. The system combines the reversible nucleic acid-binding properties of DNA-binding matrix with the speed and versatility of spin column technology to yield approximately 15-30 μg of DNA with an A260/A280 ratio of 1.7-1.9. The kit will isolate all cellular DNA, including plasmid DNA. Purified DNA is suitable for PCR, restriction digestion, and hybridization techniques. There are no organic extractions thus reducing plastic waste and hands-on time to allow multiple samples to be processed in parallel.

          Storage and Stability

          All components of the EasyPrepTM Yeast gDNA Kit, except the Proteinase K, RNase A and Lyticase can be stored at 22 -25 °C and are guaranteed for at least 24 months from the dated of purchase. Once reconstituted in water, Proteinase K and Lyticase must be stored at –20°C. Store RNase A at 4°C. Under cool ambient conditions, a precipitate may form in the Buffer YBL/YTL. In case of such an event, heat the bottle at 37°C to dissolve. Store Buffer YTL/ YBL at room temperature.

          Kit's Contents

          SKU GD05-01 GD05-02
          DNA Mini Columns
          50 250
          2 mL Collection Tubes 100 500
          Buffer YTL 20 mL 100 mL
          Buffer YBL 20 mL 100 mL
          Buffer KB 28 mL 135 mL
          DNA Wash Buffer 30 mL 3 x 36 mL
          Glass Beads 3 g 15 g
          Elution Buffer 15 mL 70 mL
          Buffer SE 30 mL 135 mL
          PK storage Buffer 5 mL 10 mL
          Lyticase (Units) 150 750
          Proteinase K 30 mg 150 mg
          RNase A 100 μL 500 μL
          Manual 1 1

           

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