The Ultrasense Pico Western Substrate, as a luminol-based enhanced chemiluminescent substrate, is sensitive and compatible with conducting immunoblots with horseradish peroxidase (HRP) – conjugated secondary antibodies. The low picogram to mid femtogram detection of antigen is enabled by Ultrasense Pico Ultra Western Substrate’s excellent sensitivity and long signal duration. Further, its long chemiluminescent signal duration makes both digital and film-based imaging possible without any loss of the signal. Appropriate primary and secondary antibody dilutions are suggested for attaining optimal signal intensity and duratio
Features:
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No optimization required. Switching to the Ultrasense Pico Western Substrate from other brands, such as Thermo Scientific™ SuperSignal™ West Dura and Millipore™ Immobilon™ substrates, does not require optimization or protocol changes.
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High degree of sensitivity and enhanced chemiluminescence duration. UltraSense Pico Western Substrate enables an accurate low picogram to mid femtogram detection of protein on the same immunoblot after a single exposure.
- Optimized for use with PVDF and nitrocellulose membranes
- Compatible with Western Blotting Markers
- Optimized for film- and CCD-based imaging.
Chemiluminescent development
- Keep the membrane moist in the wash buffer while preparing the substrate mixture. Make sure the membrane does not dry out in the next steps.
- The chemiluminescent substrate solution is sufficiently stirred to prepare the 0.1ml of solution / cm2 of the membrane.
- For a small membrane (7 x 8.5 cm), 4 ml of solution is sufficient.
- For a medium-sized membrane (8.5 x 135 cm), 10 ml of solution is sufficient.
- Place the membrane on a clean, flat surface or in a clean container.
- Remove the membrane from the chemiluminescent substrate solution and drain the excess solution.
- Place the membrane in a plastic sheet protector or plastic wrap to prevent the film from drying out.
- Imaging the membrane with a digital imager or by exposure to the X-ray film.
