A novel recombinant reverse transcriptase that exhibits much higher efficiency in the first-strand cDNA synthesis from RNA templates with secondary structures and high GC content. PowerScriptTM Plus Reverse Transcriptase is engineered to perform under high temperatures (50°C - 60°C), facilitating the elimination of secondary structures associated with GC-rich RNA templates. Due to this unique feature, ThermoScriptTM Plus can synthesize full-length cDNA libraries from RNA templates up to 12 kb in length.
Features
- Lack RNase H activity: higher yield of cDNA.
- Thermal stable: the optimum reaction temperature is 50°C, the highest is 60°C. Can overcome the template RNA secondary structure, and finish the reverse transcriptase experiment smoothly.
- Wide temperature range: can reverse transcript from 37-60°C, with more than 80% of the highest activity at 42°C-60°C. customer can choose the reaction temperature freely.
- Strong amplification activity: Gene mutation enhanced the binding capacity of the enzyme and RNA. Increased the amplification speed, can obtain the quality cDNA, suitable for cDNA library construction.
Applications
- Synthesizing cDNA from a ssRNA
- Constructing cDNA library
Source
Recombination of E. coli containing Moloney murine leukemia virus reverse transcriptase gene from clone of Moloney murine.
Unit definition
Using Poly (A) as the template and oligo (dT) as the primer, the enzyme required to catalyze the incorporation of 1 nmol of dTTP within 10 minutes at 37 oC is defined as one active unit (U).
Concentration: 200 U/ul
Size: 25 ul
Form: Enzyme supplied with 5X RT buffer
Storage: Store all components at -20°C
