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T4 DNA Polymerase (3U/ul, 50ul)

    Description

    Description

    This product is expressed by Escherichia coli, and the source of the expressed gene is T4 bacteriophage. Due to the simultaneous activity of 5'→3' DNA polymerase and 3'→5' DNA exonuclease, T4 DNA polymerase can be used to flatten the 5' protruding end or flatten the 3' protruding end. It can also be used for labeling DNA probe synthesis through displacement reactions, analyzing the starting point of mRNA transcription through primer elongation, synthesizing the second strand during site-specific mutagenesis, and cloning PCR products that do not rely on linkage reactions.The 3'→5' DNA exonuclease activity of this T4 DNA polymerase is about 100-1000 times higher than Klenow Fragment, and it has higher activity for single stranded DNA than double stranded DNA. This enzyme does not contain exonuclease activity of 5'→3' DNA, and can be inactivated by heating at 70°C for 10 minutes. Metal ion chelating agents can inhibit its activity.

    Unit definition

    The amount of enzyme required to incorporate 10 nmol of whole nucleotides into acid insoluble precipitate is defined as 1 active unit (U) within 30 minutes, using thermally denatured calf thymus DNA as a template/primer, at 37°C and pH 8.8.

    Quality control

    2U of this enzyme reacted with 1μg of Closed Circular (RFI) pBR322 DNA at 37℃ for 16 hours, and the electrophoresis bands of the DNA remained unchanged.

    Shipping and Storage

    -20°C

    Components

    SKU TDP01-01 TDP01-02
    T4 DNA Polymerase (3U/μl) 50 μl 250 μl
    10×T4 DNA Polymerase Reaction Buffer
    1 ml 4x1 ml
    Product form

    SKU: TDP01-01

    T4 DNA Polymerase (3U/ul, 50ul)

      Description This product is expressed by Escherichia coli, and the source of the expressed gene is T4 bacteriophage. Due to... Read more

      $100.00 Excl. VAT

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          Description

          Description

          This product is expressed by Escherichia coli, and the source of the expressed gene is T4 bacteriophage. Due to the simultaneous activity of 5'→3' DNA polymerase and 3'→5' DNA exonuclease, T4 DNA polymerase can be used to flatten the 5' protruding end or flatten the 3' protruding end. It can also be used for labeling DNA probe synthesis through displacement reactions, analyzing the starting point of mRNA transcription through primer elongation, synthesizing the second strand during site-specific mutagenesis, and cloning PCR products that do not rely on linkage reactions.The 3'→5' DNA exonuclease activity of this T4 DNA polymerase is about 100-1000 times higher than Klenow Fragment, and it has higher activity for single stranded DNA than double stranded DNA. This enzyme does not contain exonuclease activity of 5'→3' DNA, and can be inactivated by heating at 70°C for 10 minutes. Metal ion chelating agents can inhibit its activity.

          Unit definition

          The amount of enzyme required to incorporate 10 nmol of whole nucleotides into acid insoluble precipitate is defined as 1 active unit (U) within 30 minutes, using thermally denatured calf thymus DNA as a template/primer, at 37°C and pH 8.8.

          Quality control

          2U of this enzyme reacted with 1μg of Closed Circular (RFI) pBR322 DNA at 37℃ for 16 hours, and the electrophoresis bands of the DNA remained unchanged.

          Shipping and Storage

          -20°C

          Components

          SKU TDP01-01 TDP01-02
          T4 DNA Polymerase (3U/μl) 50 μl 250 μl
          10×T4 DNA Polymerase Reaction Buffer
          1 ml 4x1 ml

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