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RNase H (60U/ul, 10ul)

    Description

    RNase H, Recombinant is a ribonucleic acid endonuclease that specifically breaks down RNA strands in RNA DNA hybrids, and cannot digest single or double stranded DNA.This product can be used for the detection of DNA-RNA hybrids, removing mRNA during the synthesis of the second strand of cDNA, and removing the poly (A) tail of mRNA hybridized to poly (dT).

    Features

    This product is a thermally stable RNase H enzyme that still exhibits RNase H activity after incubation at 70°C or above for 10 minutes.

    Unit definition

    Using poly (rA)·poly (dT) as the substrate, the enzyme required to produce 1nmol of acid soluble substance within 20 minutes at 37°C is defined as 1 active unit (U)

    Storage Buffer

    Tris-HCl (pH7.5) 25mM，NaCl 30mM，DTT 1mM，EDTA 0.5mM，Glycerol 50%.

    Shipping and Storage

    -20°C.

    Components

    SKU RNH01-01
    RNase H, Recombinant, 60U/μl 10μl
    5×RNase H Reaction Buffer
    		 300μl

    Read more

    Product form

    SKU: RNH01-01

    RNase H (60U/ul, 10ul)

      RNase H, Recombinant is a ribonucleic acid endonuclease that specifically breaks down RNA strands in RNA DNA hybrids, and cannot... Read more

      $100.00 Excl. VAT

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          Description

          RNase H, Recombinant is a ribonucleic acid endonuclease that specifically breaks down RNA strands in RNA DNA hybrids, and cannot digest single or double stranded DNA.This product can be used for the detection of DNA-RNA hybrids, removing mRNA during the synthesis of the second strand of cDNA, and removing the poly (A) tail of mRNA hybridized to poly (dT).

          Features

          This product is a thermally stable RNase H enzyme that still exhibits RNase H activity after incubation at 70°C or above for 10 minutes.

          Unit definition

          Using poly (rA)·poly (dT) as the substrate, the enzyme required to produce 1nmol of acid soluble substance within 20 minutes at 37°C is defined as 1 active unit (U)

          Storage Buffer

          Tris-HCl (pH7.5) 25mM，NaCl 30mM，DTT 1mM，EDTA 0.5mM，Glycerol 50%.

          Shipping and Storage

          -20°C.

          Components

          SKU RNH01-01
          RNase H, Recombinant, 60U/μl 10μl
          5×RNase H Reaction Buffer
          		 300μl

          Read more

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