RNase H, Recombinant is a ribonucleic acid endonuclease that specifically breaks down RNA strands in RNA DNA hybrids, and cannot digest single or double stranded DNA.This product can be used for the detection of DNA-RNA hybrids, removing mRNA during the synthesis of the second strand of cDNA, and removing the poly (A) tail of mRNA hybridized to poly (dT).
Features
This product is a thermally stable RNase H enzyme that still exhibits RNase H activity after incubation at 70°C or above for 10 minutes.
Unit definition
Using poly (rA)·poly (dT) as the substrate, the enzyme required to produce 1nmol of acid soluble substance within 20 minutes at 37°C is defined as 1 active unit (U)
Storage Buffer
Tris-HCl (pH7.5) 25mM，NaCl 30mM，DTT 1mM，EDTA 0.5mM，Glycerol 50%.
Shipping and Storage
-20°C.
Components
| SKU |
RNH01-01 |
| RNase H, Recombinant, 60U/μl |
10μl |
5×RNase H Reaction Buffer
|
300μl |
