Green DNA Gel Stain is a new and safe class fluorescent dye, used for agarose gel or polyacylamide gel staining to visualize DNA or RNA. It is a safe replacement of Ethidium Bromide, a potent mutagen. Green DNA Gel Stain showed negative results in Ames test, indicating that it's not carcinogenic.
Green DNA Gel Stain is used the same way as Ethidium Bromide but only use LED light instead of UV light. It emits green fluorescence when bound to DNA under LED light. It has the excitation maxima: 290nm.
Protocol
- Prepare a 50 ml agarose or polyacrylamide solution.
- Add 5 ul Safe DNA Gel Stain to the gel solution.
- Mix gently; the solution should have no air bubbles.
- For agarose gel, let the solution cool down to 60 - 70oC and cast the gel. For polyacrylamide gel, add APS and TEMED and cast the gel according to regular polyacrylamide gel casting protocol.
- Run gel electrophoresis.
- View the results under blue LED light.
