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Endofree EasyFilter Plasmid Midiprep Kit (10 Preps)

    Description

    EasyPrep™ EndoFree Plasmid Midiprep Kit provides a simple, fast and cost effective method to purify plasmid DNAs from bacterial culture, when amount of the plasmid DNA needed is about 200 µg. The kit is especially convenient to use when multiple plasmids need to be purified at the same time, as the use of traditional commercial kits is time-consuming.

    Key to the kit is our proprietary DNA binding systems that allow the high efficient DNA binding  while proteins and other impurities are removed by wash buffer. Nucleic acids are easily eluted with Elution Buffer. Our proprietary endotoxin removal buffer, Buffer RET, is designed to remove endotoxin by a single washing step without tedious phase partitioning steps. The purified plasmid DNA, with endotoxin level less than 0.1 EU per µg of DNA, is ready for transfection of endotoxin sensitive cell lines and microinjections.

    Kit's Contents

    SKU PD03-21
    PD03-31
    PD03-22
    PD03-32
    Midiprep DNA Columns 10 25
    EasyFilter 10
    (PD03-31)
    25
    (PD03-32)
    Buffer A1 30 ml 75 ml
    Buffer B1 30 ml 75 ml
    Buffer N3 15 ml 30 ml
    Buffer RET 30 ml 75 ml
    DNA Wash Buffer (5x)* 25 ml 50 ml
    EndoFree Elution Buffer 10 ml 20 ml
    RNase A 110 µl 250 µl
    Manual 1 1

    * Add ethanol to washing buffer before use

    Product form

    SKU: PD03-31

    Endofree EasyFilter Plasmid Midiprep Kit (10 Preps)

      EasyPrep™ EndoFree Plasmid Midiprep Kit provides a simple, fast and cost effective method to purify plasmid DNAs from bacterial culture,... Read more

      $95.00 Excl. VAT

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            Instruction Manual

          Description

          EasyPrep™ EndoFree Plasmid Midiprep Kit provides a simple, fast and cost effective method to purify plasmid DNAs from bacterial culture, when amount of the plasmid DNA needed is about 200 µg. The kit is especially convenient to use when multiple plasmids need to be purified at the same time, as the use of traditional commercial kits is time-consuming.

          Key to the kit is our proprietary DNA binding systems that allow the high efficient DNA binding  while proteins and other impurities are removed by wash buffer. Nucleic acids are easily eluted with Elution Buffer. Our proprietary endotoxin removal buffer, Buffer RET, is designed to remove endotoxin by a single washing step without tedious phase partitioning steps. The purified plasmid DNA, with endotoxin level less than 0.1 EU per µg of DNA, is ready for transfection of endotoxin sensitive cell lines and microinjections.

          Kit's Contents

          SKU PD03-21
          PD03-31
          PD03-22
          PD03-32
          Midiprep DNA Columns 10 25
          EasyFilter 10
          (PD03-31)
          25
          (PD03-32)
          Buffer A1 30 ml 75 ml
          Buffer B1 30 ml 75 ml
          Buffer N3 15 ml 30 ml
          Buffer RET 30 ml 75 ml
          DNA Wash Buffer (5x)* 25 ml 50 ml
          EndoFree Elution Buffer 10 ml 20 ml
          RNase A 110 µl 250 µl
          Manual 1 1

          * Add ethanol to washing buffer before use

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