Introduction
EasyMag® Viral/Pathogen DNA/RNA Kit is designed to use magnetic bead-based technology for rapid and reliable isolation of total nucleic acids from viruses, bacteria, and yeast in whole blood, serum, plasma, swabs, saliva, urine, and other bodily fluids. The kit combines the enhanced lysis/binding buffer formulation and reversible nucleic acid- binding properties of magnetic particles that provides the highest sensitivity in viral/pathogen DNA/RNA recovery. Viral/Pathogen DNA or RNA extraction can be performed manually or on automated platforms with equivalent results. The isolated viral DNA or RNA can be directly used in downstream applications such as amplification and enzymatic reactions without the need for further purification.
Features:
- Optimized for isolation from bacterial and viral samples.
- Magnetic bead-based chemistry with no centrifugation or filtration
- Rapid and reliable purification of nucleic acids
- Adaptable to various automated liquid handling workstations
Kit Contents
| SKU |
GDR05-02 |
GDR05-03 |
| Preps |
200 |
2000 |
| Magnetic Beads |
2.2 ml |
22 ml |
| VDR Buffer |
100 ml |
1000 ml |
| DRW1 Buffer* |
100 ml |
1000 ml |
| DRW2 Buffer* |
2x 40 ml |
2x 400 ml |
| RNA Elution Buffer |
100 ml |
1000 ml |
| Proteinase K |
4.4 ml |
44 ml |
| LEM-1 ** |
4.4 ml |
44 ml |
*Ethanol must be added prior to use. See Preparation of Reagents
** Lysis Enzyme Mix 1 (LEM-1) is stable at 2-8°C (3 months). Keep at -20°C.
Storage and Stability
The kit is shipped at ambient temperature. All components are stable for 12 months when stored accordingly. Magnetic Beads, Proteinase K Solution can be stored at room temperature (15-25°C) for 12 months, to prolong the shelf-life, storage at 2-8°C is recommended. LEM I are stable at 2-8°C (3 months). For longer storage, keep at -20°C. All other components can be stored at room temperature.
Manual Protocol
Automation Protocol
