EasyPrep™ Plasmid Midiprep-II Kit provides a simple, fast and cost effective method to purify plasmid DNAs from bacterial culture, when amount of the plasmid DNA needed is about 400-500 µg. The kit is especially convenient to use when multiple plasmids need to be purified at the same time, as the use of traditional commercial kits is time-consuming. EasyPrepTM Plasmid Midiprep-II Kit applies DNA-binding-specific matrix, which allows the high efficient binding of DNA, while proteins and other cellular components are easily removed during washing. Plasmid DNAs are easily eluted with Tris buffer or pure water.
Plasmid DNAs purified from EasyPrep™ Plasmid Midiprep-II Kit can be used directly for downstream applications such as transfection, restriction mapping, library screening, sequencing, as well as gene therapy and genetic vaccinations.
Kit's Contents
| SKU |
PD04-01
PD04-11 |
PD04-02
PD04-12 |
| Midiprep DNA Columns |
10 |
25 |
| EasyFilter |
10
(PD04-11) |
25
(PD04-12) |
| Buffer A1 |
60 ml |
150 ml |
| Buffer A2 |
60 ml |
150 ml |
| Buffer A3 |
75 ml |
180 ml |
| Elution Buffer |
10 ml |
20 ml |
| Column Buffer |
35 ml |
80 ml |
| RNase A |
210 µl |
520 µl |
| 50ml conical tubes |
10 |
25 |
| Manual |
1 |
1 |
