BioE Electroporation Solution provides a convenient and economical way to transfect primary and suspension cells. It provides excellent transfection efficiencies and cell viabilities during electroporation of DNA and RNA. This solution can be used with a broad range of cells and is compatible with many square wave or exponential decay wave electroporators.
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Recommended electroporation conditions for select cell types using Square Wave Systems
|
Cell Line
|
Nucleic Acid
|
Voltage (V)
|
Pulse Duration (msec)
|
Cuvette
|
|
A549
|
Plasmid or siRNA
|
160
|
15
|
2 mm
|
|
CHO
|
Plasmid or siRNA
|
160
|
15
|
2 mm
|
|
CV1
|
Plasmid or siRNA
|
100
|
25
|
2 mm
|
|
BHK21
|
Plasmid or siRNA
|
140
|
25
|
2 mm
|
|
HL60
|
Plasmid or siRNA
|
140
|
25
|
2 mm
|
|
HuT78
|
Plasmid or siRNA
|
130
|
25
|
2 mm
|
- Recommended electroporation conditions for select cell types using Exponential Decay Wave Systems
|
Cell Line
|
Nucleic Acid
|
Voltage (V)
|
Capacitance (µF)
|
Resistance (ohms)
|
Cuvette
|
|
HeLa
|
Plasmid or siRNA
|
160
|
500
|
¥
|
2 mm
|
|
Jurkat
|
Plasmid or siRNA
|
140
|
1000
|
¥
|
2 mm
|
|
K562
|
Plasmid or siRNA
|
155
|
1000
|
¥
|
2 mm
|
|
3T3
|
Plasmid or siRNA
|
160
|
500
|
¥
|
2 mm
|
