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BioE, DNA/RNA Electroporation solution (15 ml)

    Description

    BioE Electroporation Solution provides a convenient and economical way to transfect primary and suspension cells. It provides excellent transfection efficiencies and cell viabilities during electroporation of DNA and RNA. This solution can be used with a broad range of cells and is compatible with many square wave or exponential decay wave electroporators.

    • Recommended electroporation conditions for select cell types using Square Wave Systems

    Cell Line

    Nucleic Acid

    Voltage (V)

    Pulse Duration (msec)

    Cuvette

    A549

    Plasmid or siRNA

    160

    15

    2 mm

    CHO

    Plasmid or siRNA

    160

    15

    2 mm

    CV1

    Plasmid or siRNA

    100

    25

    2 mm

    BHK21

    Plasmid or siRNA

    140

    25

    2 mm

    HL60

    Plasmid or siRNA

    140

    25

    2 mm

    HuT78

    Plasmid or siRNA

    130

    25

    2 mm

    • Recommended electroporation conditions for select cell types using Exponential Decay Wave Systems

    Cell Line

    Nucleic Acid

    Voltage 

    (V)

    Capacitance (µF)

    Resistance (ohms)

    Cuvette

    HeLa

    Plasmid or siRNA

    160

    500

    ¥

    2 mm

    Jurkat

    Plasmid or siRNA

    140

    1000

    ¥

    2 mm

    K562

    Plasmid or siRNA

    155

    1000

    ¥

    2 mm

    3T3

    Plasmid or siRNA

    160

    500

    ¥

    2 mm

     

    Product form

    SKU: B02-02

    BioE, DNA/RNA Electroporation solution (15 ml)

      BioE Electroporation Solution provides a convenient and economical way to transfect primary and suspension cells. It provides excellent transfection efficiencies... Read more

      $495.00

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            Protocols

          Description

          BioE Electroporation Solution provides a convenient and economical way to transfect primary and suspension cells. It provides excellent transfection efficiencies and cell viabilities during electroporation of DNA and RNA. This solution can be used with a broad range of cells and is compatible with many square wave or exponential decay wave electroporators.

          • Recommended electroporation conditions for select cell types using Square Wave Systems

          Cell Line

          Nucleic Acid

          Voltage (V)

          Pulse Duration (msec)

          Cuvette

          A549

          Plasmid or siRNA

          160

          15

          2 mm

          CHO

          Plasmid or siRNA

          160

          15

          2 mm

          CV1

          Plasmid or siRNA

          100

          25

          2 mm

          BHK21

          Plasmid or siRNA

          140

          25

          2 mm

          HL60

          Plasmid or siRNA

          140

          25

          2 mm

          HuT78

          Plasmid or siRNA

          130

          25

          2 mm

          • Recommended electroporation conditions for select cell types using Exponential Decay Wave Systems

          Cell Line

          Nucleic Acid

          Voltage 

          (V)

          Capacitance (µF)

          Resistance (ohms)

          Cuvette

          HeLa

          Plasmid or siRNA

          160

          500

          ¥

          2 mm

          Jurkat

          Plasmid or siRNA

          140

          1000

          ¥

          2 mm

          K562

          Plasmid or siRNA

          155

          1000

          ¥

          2 mm

          3T3

          Plasmid or siRNA

          160

          500

          ¥

          2 mm

           

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