The EasyPrepTM Bacterial gDNA kit provides a rapid and reliable method for isolating high-quality total cellular DNA from a wide variety of bacterial species. Bacterial cells are grown to log-phase and harvested. The bacterial cell wall is digested by lysozyme and Proteinase K. Following lysis, binding conditions are adjusted and the sample is applied to a DNA column.
Key to this kit is our proprietary DNA binding system that allows the high efficient binding of DNA to the membrane while proteins and other contaminates are removed under optimal conditions. Nucleic acids are easily eluted with sterile water or Elution Buffer. Purified DNA is suitable for PCR, restriction digestion, and hybridization techniques. Each DNA column can bind approximately 100 μg genomic DNA and process up to 1 x 109 bacterial cells. The system combines the reversible nucleic acid-binding properties of membrane matrix with the speed and versatility of spin column technology to yield up to approximately 15-30 μg of DNA with an OD260/280 ratio of 1.7-1.9. The kit will isolate all cellular DNA, including plasmid DNA.
Kit's Contents
| SKU |
GD04-01 |
GD04-02 |
DNA Mini Columns
|
50 |
250 |
| 2 mL Collection Tubes |
100 |
500 |
| Buffer TL |
15 ml |
70 ml |
| Buffer BL |
15 ml |
70 ml |
| Buffer KB |
28 ml |
135 ml |
| DNA Wash Buffer |
15 ml |
3x24ml |
| Glass Beads |
1.5 g |
7 g |
| Elution Buffer |
15 ml |
60 ml |
| Lysozyme |
50 mg |
5x 50 mg |
| Proteinase K |
30 mg |
5x30mg |
| RNase A (20mg/ml) |
270 µl |
1.4 ml |
| Manual |
1 |
1 |
