The virus purification kit is designed for fast and efficient purification of recombinant Adenovirus-associated viruses from transfected cell culture supernatant of 1-2 T75 flasks. Up to 1x1013 viral particles could be purified. The elution buffer could be exchanged with any proper buffer and virus could be concentrated by using de-salting column, which is included.
Important: each purification column is used for 2 preps with the same virus.
Kit's Contents
| SKU |
AAV02-00 |
AAV02-01 |
AAV02-02 |
Store @ |
Preps
|
2 |
10 |
20 |
- |
| AAV Purification Columns |
1 |
5 |
10 |
4°C
|
| Press-On Cap |
1 |
5 |
10 |
RT |
| Centrifugal Filter (desalting column) |
1 |
5 |
10 |
RT |
| 15 ml Collection Tube |
2 |
10 |
20 |
RT |
| AAV Binding Buffer |
30 ml |
200 ml |
400 ml |
RT |
| AAV Elution Buffer |
10 ml |
50 ml |
100 ml |
RT |
| Regeneration Buffer |
10 ml |
30 ml |
100 ml |
RT |
| 100xNuclease Reaction Buffer |
100 μl |
500 μl |
1000 μl |
4°C
|
| Nuclease 25u/μl |
12 μl |
55 μl |
110 μl |
-20°C |
