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6x Tris-Glycine SDS Sample buffer (50 ml)

    Description

    Tris-Glycine SDS Sample Buffer (6x) is used to prepare protein samples for denaturing gel electrophoresis using Tris-Glycine gels. It has a pH of 6.8 and contains bromophenol blue as a tracking dye.

    To use: Heat the sample in a 1X dilution (reduced or non-reduced) at 85°C for 2–5 minutes for optimal results.

    Note: Heating samples at 100°C in SDS-containing buffers results in proteolysis.

    Composition: 375 mM Tris.HCl, 9% SDS, 50% glycerol, 0.015% bromophenol blue (Non-reduced). Add ß-mercaptoethanol (9% V/V) before use (Reduced).

    MSDS

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    SKU: SAB03-02

    6x Tris-Glycine SDS Sample buffer (50 ml)

      Tris-Glycine SDS Sample Buffer (6x) is used to prepare protein samples for denaturing gel electrophoresis using Tris-Glycine gels. It has... Read more

      $40.00 $30.00

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          Description

          Tris-Glycine SDS Sample Buffer (6x) is used to prepare protein samples for denaturing gel electrophoresis using Tris-Glycine gels. It has a pH of 6.8 and contains bromophenol blue as a tracking dye.

          To use: Heat the sample in a 1X dilution (reduced or non-reduced) at 85°C for 2–5 minutes for optimal results.

          Note: Heating samples at 100°C in SDS-containing buffers results in proteolysis.

          Composition: 375 mM Tris.HCl, 9% SDS, 50% glycerol, 0.015% bromophenol blue (Non-reduced). Add ß-mercaptoethanol (9% V/V) before use (Reduced).

          MSDS

          Read more

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