6x Tris-Glycine Native Sample Buffer (25ml)

Description

Description

Tris-Glycine Native Sample Buffer (6x) is used to prepare protein samples for native gel electrophoresis using Tris-Glycine gels or Tris-Acetate gels. It has a pH of 6.8 and contains bromophenol blue as a tracking dye.

To use: Heat the sample in a 1X dilution (reduced or non-reduced) at 85°C for 2–5 minutes for optimal results.

Note: Heating samples at 100°C in SDS-containing buffers results in proteolysis.

Composition: 600mM Tris.HCl, 50% glycerol, 0.02% bromophenol blue (Non-reduced). Add ß-mercaptoethanol (9% V/V) before use (Reduced).

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SKU: SABN03-01

6x Tris-Glycine Native Sample Buffer (25ml)

DescriptionTris-Glycine Native Sample Buffer (6x) is used to prepare protein samples for native gel electrophoresis using Tris-Glycine gels or Tris-Acetate gels.... Read more

$25.00 Excl. VAT

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    Description

    Description

    Tris-Glycine Native Sample Buffer (6x) is used to prepare protein samples for native gel electrophoresis using Tris-Glycine gels or Tris-Acetate gels. It has a pH of 6.8 and contains bromophenol blue as a tracking dye.

    To use: Heat the sample in a 1X dilution (reduced or non-reduced) at 85°C for 2–5 minutes for optimal results.

    Note: Heating samples at 100°C in SDS-containing buffers results in proteolysis.

    Composition: 600mM Tris.HCl, 50% glycerol, 0.02% bromophenol blue (Non-reduced). Add ß-mercaptoethanol (9% V/V) before use (Reduced).

    Read more

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