Ethium bromide replacement!
6x Red Fluorescent DNA Loading Dye is formulated by mixing fluorescent dye with standard DNA loading dye and is used by adding to DNA samples directly. There is no need to add safe DNA gel stain or other fluorescent dye to the gel or running buffer. After the electrophoresis, the red DNA bands could be viewed under UV light.
Specifications
| Storage |
Store at 4 °C. |
| Shelf life |
Two years from date of shipping. |
| Safety |
Non-carcinogenic by the ames test. May cause skin and eye irritation. Always wear gloves when working with the product. |
| Disposal |
Dispose as you would any other non-carcinogenic fluorescent dye (eg. Acridine orange; Propidium iodide). |
Protocol
- Prepare a 100 ml agarose or polyacrylamide solution with no stain. Mix gently to avoid bubbles.
- For agarose gels, let the solution cool down to 60 - 70 oC before casting the gel. For polyacrylamide gel, add APS and TEMED and cast the gel according to regular protocol.
- Mix samples or DNA marker with SafeRed dye at a 1:5 (dye : sample) dilution rate.
- Following electrophoresis, view the results under UV.
