The XL 1kb Plus DNA Ladder is a ready-to-use DNA ladder, which is pre-mixed with loading dye for direct loading on gel. The DNA Ladder is composed of 14 individual DNA fragments: 25k, 10k, 8k, 6k, 5k, 4k, 3k, 2.5k, 2k, 1.5k, 1k, 750, 500, and 250 base pairs derived from a mixture of PCR products and specifically digested plasmid DNA. This product contains two enhanced bands (3 kb and 1 kb) for easy referencing. In addition, two tracking dyes, Xylene cyanol FF and Bromophenol blue which mimic the migration of 4,000 bp and 500 bp dsDNA during electrophoresis are also added for real time monitoring.
Source
Phenol extracted PCR products and dsDNA digested with specific restriction enzymes, equilibrated in 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA.
Range
XL 1kb Plus: 100 ~ 25,000 bp
Concentration
XL 1kb Plus: 51.6 µg/ 500 µl
Recommended loading volume
5 µl/ well
Storage
Protected from light
Room temperature ≥ 6 months
4°C ≥ 12 months
-20°C ≥ 36 months
