4x Tris-Glycine SDS Sample Buffer (50 ml)

Description

Tris-Glycine SDS Sample Buffer (4x) is used to prepare protein samples for denaturing gel electrophoresis using Tris-Glycine gels. It has a pH of 6.8 and contains bromophenol blue as a tracking dye.

To use: Heat the sample in a 1X dilution (reduced or non-reduced) at 85°C for 2–5 minutes for optimal results.

Note: Heating samples at 100°C in SDS-containing buffers results in proteolysis.

Composition: 250 mM Tris HCl, 8% SDS, 40% Glycerol, 0.01% Bromophenol blue (Non-reduced). Add ß-mercaptoethanol (8% V/V) before use (Reduced).  

MSDS

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Product form

SKU: SAB02-02

4x Tris-Glycine SDS Sample Buffer (50 ml)

Tris-Glycine SDS Sample Buffer (4x) is used to prepare protein samples for denaturing gel electrophoresis using Tris-Glycine gels. It has... Read more

$30.00 Excl. VAT

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    Description

    Tris-Glycine SDS Sample Buffer (4x) is used to prepare protein samples for denaturing gel electrophoresis using Tris-Glycine gels. It has a pH of 6.8 and contains bromophenol blue as a tracking dye.

    To use: Heat the sample in a 1X dilution (reduced or non-reduced) at 85°C for 2–5 minutes for optimal results.

    Note: Heating samples at 100°C in SDS-containing buffers results in proteolysis.

    Composition: 250 mM Tris HCl, 8% SDS, 40% Glycerol, 0.01% Bromophenol blue (Non-reduced). Add ß-mercaptoethanol (8% V/V) before use (Reduced).  

    MSDS

    Read more

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