4x Tris Glycine Native Sample Buffer (25 ml)

Description

Tris-Glycine Native Sample Buffer (4x) is used to prepare protein samples for native gel electrophoresis using Tris-Glycine gels or Tris-Acetate gels. It has a pH of 6.8 and contains bromophenol blue as a tracking dye.

To use: Heat the sample in a 1x dilution (reduced or non-reduced) at 85°C for 2–5 minutes for optimal results.

Note: Heating samples at 100°C in SDS-containing buffers results in proteolysis.

Composition: 400 mM Tris HCl, 40% Glycerol, 0.02% Bromophenol blue (Non-reduced). Add ß-mercaptoethanol (8% V/V) before use (Reduced). 

MSDS

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Product form

SKU: SABN02-01

4x Tris Glycine Native Sample Buffer (25 ml)

Tris-Glycine Native Sample Buffer (4x) is used to prepare protein samples for native gel electrophoresis using Tris-Glycine gels or Tris-Acetate... Read more

$25.00 Excl. VAT

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    Description

    Tris-Glycine Native Sample Buffer (4x) is used to prepare protein samples for native gel electrophoresis using Tris-Glycine gels or Tris-Acetate gels. It has a pH of 6.8 and contains bromophenol blue as a tracking dye.

    To use: Heat the sample in a 1x dilution (reduced or non-reduced) at 85°C for 2–5 minutes for optimal results.

    Note: Heating samples at 100°C in SDS-containing buffers results in proteolysis.

    Composition: 400 mM Tris HCl, 40% Glycerol, 0.02% Bromophenol blue (Non-reduced). Add ß-mercaptoethanol (8% V/V) before use (Reduced). 

    MSDS

    Read more

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