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2xqPCR Master Mix (No ROX)

    Description

    SYBR Green I-based qPCR reagent:

    • It contains PowerPCR™ Hot Start Taq DNA polymerase, PCR buffer, dNTPs, SYBR Green I fluorescent dyes and Mg2+.
    • It can be applied to the target sequence detection of genomic DNA and cDNA. The SYBR Green I dye binds to double-stranded (ds) DNA without using sequence-specific probes.
    • The chemically modified PowerPCR™ Hot Start Taq DNA polymerase is inactive at room temperature which effectively avoids the nonspecific amplification caused by primer dimers or the nonspecific binding of prime and template. The enzyme should be activated by incubation at 95°C for 10 minutes.
    • The unique PCR buffer components and the hot start enzyme ensure PCR specificity and sensitivity.
    • It has a higher sensitivity and a wider linear range which can exactly quantitate the target gene or the low copy template.

    Read more

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    SKU: QP01-00

    2xqPCR Master Mix (No ROX)

      SYBR Green I-based qPCR reagent: It contains PowerPCR™ Hot Start Taq DNA polymerase, PCR buffer, dNTPs, SYBR Green I fluorescent... Read more

      $50.00

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          Description

          SYBR Green I-based qPCR reagent:

          • It contains PowerPCR™ Hot Start Taq DNA polymerase, PCR buffer, dNTPs, SYBR Green I fluorescent dyes and Mg2+.
          • It can be applied to the target sequence detection of genomic DNA and cDNA. The SYBR Green I dye binds to double-stranded (ds) DNA without using sequence-specific probes.
          • The chemically modified PowerPCR™ Hot Start Taq DNA polymerase is inactive at room temperature which effectively avoids the nonspecific amplification caused by primer dimers or the nonspecific binding of prime and template. The enzyme should be activated by incubation at 95°C for 10 minutes.
          • The unique PCR buffer components and the hot start enzyme ensure PCR specificity and sensitivity.
          • It has a higher sensitivity and a wider linear range which can exactly quantitate the target gene or the low copy template.

          Read more

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