2xqPCR Master Mix (High ROX)

Description

SYBR Green I-based qPCR reagent:

  • It contains PowerPCR™ Hot Start Taq DNA polymerase, PCR buffer, dNTPs, SYBR Green I fluorescent dyes, Mg2+ and ROX calibration dye.
  • It can be applied to the target sequence detection of genomic DNA and cDNA. The SYBR Green I dye binds to dsDNA without using sequence-specific probes.
  • The chemically modified PowerPCR™ Hot Start Taq DNA polymerase is inactive at room temperature which effectively avoids the non-specific amplification caused by primer dimers or the nonspecific binding of prime and template. The enzyme should be activated by incubation at 95°C for 10 minutes.
  • The unique PCR buffer components and the Hot start enzyme ensure PCR specificity and sensitivity.
  • The ROX is a dye molecule that can be used to normalize the well-to-well fluorescent fluctuations and can be applied to all qPCR instruments using ROX as calibration dye.
  • It has a higher sensitivity and a wider linear range which can exactly quantitate the target gene or low copy template.

Suitable Real-Time PCR detection systems:

  • ABI Prism7000/7300/7700/7900
  • ABI Step One/StepOne Plus
  • Eppendorf
  • systems need high ROX signals

Read more

Product form

SKU: QP03-00

2xqPCR Master Mix (High ROX)

SYBR Green I-based qPCR reagent: It contains PowerPCR™ Hot Start Taq DNA polymerase, PCR buffer, dNTPs, SYBR Green I fluorescent... Read more

$50.00 Excl. VAT

    • Shipped today? Order within: Sep 15, 2025 15:00:00 -0700
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    Description

    SYBR Green I-based qPCR reagent:

    • It contains PowerPCR™ Hot Start Taq DNA polymerase, PCR buffer, dNTPs, SYBR Green I fluorescent dyes, Mg2+ and ROX calibration dye.
    • It can be applied to the target sequence detection of genomic DNA and cDNA. The SYBR Green I dye binds to dsDNA without using sequence-specific probes.
    • The chemically modified PowerPCR™ Hot Start Taq DNA polymerase is inactive at room temperature which effectively avoids the non-specific amplification caused by primer dimers or the nonspecific binding of prime and template. The enzyme should be activated by incubation at 95°C for 10 minutes.
    • The unique PCR buffer components and the Hot start enzyme ensure PCR specificity and sensitivity.
    • The ROX is a dye molecule that can be used to normalize the well-to-well fluorescent fluctuations and can be applied to all qPCR instruments using ROX as calibration dye.
    • It has a higher sensitivity and a wider linear range which can exactly quantitate the target gene or low copy template.

    Suitable Real-Time PCR detection systems:

    • ABI Prism7000/7300/7700/7900
    • ABI Step One/StepOne Plus
    • Eppendorf
    • systems need high ROX signals

    Read more

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