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2xqPCR Master Mix (High ROX)

    Description

    SYBR Green I-based qPCR reagent:

    • It contains PowerPCR™ Hot Start Taq DNA polymerase, PCR buffer, dNTPs, SYBR Green I fluorescent dyes, Mg2+ and ROX calibration dye.
    • It can be applied to the target sequence detection of genomic DNA and cDNA. The SYBR Green I dye binds to dsDNA without using sequence-specific probes.
    • The chemically modified PowerPCR™ Hot Start Taq DNA polymerase is inactive at room temperature which effectively avoids the non-specific amplification caused by primer dimers or the nonspecific binding of prime and template. The enzyme should be activated by incubation at 95°C for 10 minutes.
    • The unique PCR buffer components and the Hot start enzyme ensure PCR specificity and sensitivity.
    • The ROX is a dye molecule that can be used to normalize the well-to-well fluorescent fluctuations and can be applied to all qPCR instruments using ROX as calibration dye.
    • It has a higher sensitivity and a wider linear range which can exactly quantitate the target gene or low copy template.

    Suitable Real-Time PCR detection systems:

    • ABI Prism7000/7300/7700/7900
    • ABI Step One/StepOne Plus
    • Eppendorf
    • systems need high ROX signals

    Read more

    Product form

    SKU: QP03-00

    2xqPCR Master Mix (High ROX)

      SYBR Green I-based qPCR reagent: It contains PowerPCR™ Hot Start Taq DNA polymerase, PCR buffer, dNTPs, SYBR Green I fluorescent... Read more

      $50.00

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          Description

          SYBR Green I-based qPCR reagent:

          • It contains PowerPCR™ Hot Start Taq DNA polymerase, PCR buffer, dNTPs, SYBR Green I fluorescent dyes, Mg2+ and ROX calibration dye.
          • It can be applied to the target sequence detection of genomic DNA and cDNA. The SYBR Green I dye binds to dsDNA without using sequence-specific probes.
          • The chemically modified PowerPCR™ Hot Start Taq DNA polymerase is inactive at room temperature which effectively avoids the non-specific amplification caused by primer dimers or the nonspecific binding of prime and template. The enzyme should be activated by incubation at 95°C for 10 minutes.
          • The unique PCR buffer components and the Hot start enzyme ensure PCR specificity and sensitivity.
          • The ROX is a dye molecule that can be used to normalize the well-to-well fluorescent fluctuations and can be applied to all qPCR instruments using ROX as calibration dye.
          • It has a higher sensitivity and a wider linear range which can exactly quantitate the target gene or low copy template.

          Suitable Real-Time PCR detection systems:

          • ABI Prism7000/7300/7700/7900
          • ABI Step One/StepOne Plus
          • Eppendorf
          • systems need high ROX signals

          Read more

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