1xTG: Tris 25 mM, Glycine 192 mM, pH 8.3, with 20% methanol. Western blot transfer buffer
Application:
For Western blotting and gel electrophoresis. Tris-glycine buffer is used to make a Tris-glycine-methanol transfer buffer, which is the most common protein transfer buffer for wet blot transfers. The methanol prevents the gel from swelling during the transfer and enhances the protein binding to membranes.
Usage Recommendations:
- To prepare 1X TG transfer buffer: add 100 ml of 10X TG buffer to 700 ml of deionized water, then add 200 ml methanol and mix well.
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Use fresh 1X TG transfer buffer. A sufficient amount of transfer buffer should be made to cover the electrode wires in the wet blot transfer unit and to soak the gel, membrane and blotting paper.
MSDS
