10xTris-Glycine Transfer Buffer (4 L)

    Description

    1xTG: Tris 25 mM, Glycine 192 mM, pH 8.3, with 20% methanol. Western blot transfer buffer

    Application:

    For Western blotting and gel electrophoresis. Tris-glycine buffer is used to make a Tris-glycine-methanol transfer buffer, which is the most common protein transfer buffer for wet blot transfers. The methanol prevents the gel from swelling during the transfer and enhances the protein binding to membranes.

    Usage Recommendations:

    • To prepare 1X TG transfer buffer:  add 100 ml of 10X TG buffer to 700 ml of deionized water, then add 200 ml methanol and mix well. 
    • Use fresh 1X TG transfer buffer. A sufficient amount of transfer buffer should be made to cover the electrode wires in the wet blot transfer unit and to soak the gel, membrane and blotting paper.

    MSDS

    Read more

    Product form

    SKU: TG01-4L

    10xTris-Glycine Transfer Buffer (4 L)

      1xTG: Tris 25 mM, Glycine 192 mM, pH 8.3, with 20% methanol. Western blot transfer buffer Application: For Western blotting and... Read more

      $68.20

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          MSDS

        Description

        1xTG: Tris 25 mM, Glycine 192 mM, pH 8.3, with 20% methanol. Western blot transfer buffer

        Application:

        For Western blotting and gel electrophoresis. Tris-glycine buffer is used to make a Tris-glycine-methanol transfer buffer, which is the most common protein transfer buffer for wet blot transfers. The methanol prevents the gel from swelling during the transfer and enhances the protein binding to membranes.

        Usage Recommendations:

        • To prepare 1X TG transfer buffer:  add 100 ml of 10X TG buffer to 700 ml of deionized water, then add 200 ml methanol and mix well. 
        • Use fresh 1X TG transfer buffer. A sufficient amount of transfer buffer should be made to cover the electrode wires in the wet blot transfer unit and to soak the gel, membrane and blotting paper.

        MSDS

        Read more

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