10x Tris-Glycine SDS Running Buffer (10 L)

Description

10xTris-Glycine SDS Running Buffer is formulated for separation of proteins in their denatured state on Tris-Glycine gels. Tris-Glycine gels provide reproducible separation of a wide range of proteins into well-resolved bands.

Composition:

10xTGS: Tris 250 mM, Glycine 1.92 M, 1%SDS, pH 8.3, Western blot running buffer

Application:

Tris-glycine-SDS (TGS) running buffer is the most commonly used buffer for sodium dodecyl sulfate – polyacrylamide gel electrophoresis (SDS-PAGE) of proteins. TGS is usually used for both the anode buffer and the cathode buffer. Recommended running conditions is 150 volts for mini vertical gel electrophoresis units.

Usage Recommendations

To prepare 1X TGS buffer, add 100 ml of 10X TGS buffer to 900 ml of deionized water and mix well. 

MSDS

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Product form

SKU: TGS01-10L

10x Tris-Glycine SDS Running Buffer (10 L)

10xTris-Glycine SDS Running Buffer is formulated for separation of proteins in their denatured state on Tris-Glycine gels. Tris-Glycine gels provide reproducible... Read more

$129.00 Excl. VAT

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    Description

    10xTris-Glycine SDS Running Buffer is formulated for separation of proteins in their denatured state on Tris-Glycine gels. Tris-Glycine gels provide reproducible separation of a wide range of proteins into well-resolved bands.

    Composition:

    10xTGS: Tris 250 mM, Glycine 1.92 M, 1%SDS, pH 8.3, Western blot running buffer

    Application:

    Tris-glycine-SDS (TGS) running buffer is the most commonly used buffer for sodium dodecyl sulfate – polyacrylamide gel electrophoresis (SDS-PAGE) of proteins. TGS is usually used for both the anode buffer and the cathode buffer. Recommended running conditions is 150 volts for mini vertical gel electrophoresis units.

    Usage Recommendations

    To prepare 1X TGS buffer, add 100 ml of 10X TGS buffer to 900 ml of deionized water and mix well. 

    MSDS

    Read more

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