| Name | 1x Composition | Applications |
| 10x PBS | Phosphate buffer 10 mM, KCl 2.7 mM, NaCl 137 mM, pH 7.4 | Most widely used washing buffer for Western blot, ELISA, Immuno-Histochemistry staining et al. |
| 10x PBST | Phosphate buffer 10 mM, KCl 2.7 mM, NaCl 137 mM, Tween-20 0.05%, pH 7.4 | Western blot, ELISA, Immuno-Histochemical staining et. al. |
| 10x TBS | TBS01: Tris 50 mM, NaCl 150 mM, pH 7.4 | Western blot, ELISA, Immuno-Histochemistry |
| 20x TBS | TBS03, TBS04: Tris 25 mM, NaCl 137 mM, KCl 2.7 mM, pH 7.6 | Western blot, ELISA, Immuno-Histochemistry |
| 10x TBST | TBST01: Tris 50 mM, NaCl 150 mM, Tween-20 0.05%, pH 7.4 | Western blot, ELISA, Immuno-Histochemistry |
| 20x TBST | TBST03, TBST04: Tris 25mM, NaCl 137mM, KCl 2.7mM, Tween 0.05%, pH 7.6 | Western blot, ELISA, Immuno-Histochemistry |
Washing Buffers
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10xTBST Buffer (1 L)
SKU: TBST01-1LTRIS-buffered saline with Tween-20 (10x TBST) is used as a washing buffer for Western blot and other immunoassays, such as ELISA and dot blot assay. It acts as a pH stabilizer, which enable washing without disruption of antibody-antigen binding interactions. Tween 20 reduces nonspecific binding and protein: protein interactions. Each 10X TBST solution is ready to use upon dilution to the desired concentration. Product Specification Appearance Clear, Colorless Form Liquid pH 7.4 ± 0.2 Composition (1x) 50mM Tris, 150mM NaCl, 0.05% Tween-20 Comment All components except Tween-20 are dissolved in ultrapure (Type I) water, followed by pH adjustment and filtered through 0.45 micron filter. Tween-20 is added and mixed before shipment. MSDS
$25.90
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10xTBST Buffer (4 L)
SKU: TBST01-4LTRIS-buffered saline with Tween-20 (10x TBST) is used as a washing buffer for Western blot and other immunoassays, such as ELISA and dot blot assay. It acts as a pH stabilizer, which enable washing without disruption of antibody-antigen binding interactions. Tween 20 reduces nonspecific binding and protein: protein interactions. Each 10X TBST solution is ready to use upon dilution to the desired concentration. Product Specification Appearance Clear, Colorless Form Liquid pH 7.4 ± 0.2 Composition (1x) 50mM Tris, 150mM NaCl, 0.05% Tween-20 Comment All components except Tween-20 are dissolved in ultrapure (Type I) water, followed by pH adjustment and filtered through 0.45 micron filter. Tween-20 is added and mixed before shipment. MSDS
$80.00
