20X SSPE is a prepared buffer concentrate for use in nucleic acid hybridizations and blot transfer applications. Specifications Form: Liquid Concentration: 20 X Application: Nucleic Acid Gel Electrophoresis, Blotting Shipping Condition: Room Temperature Composition 3.0 M NaCl 0.2 M NaH2PO4 0.02 M EDTA pH 7.4. Storage: Room Temperature MSDS

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20X SSPE is a prepared buffer concentrate for use in nucleic acid hybridizations and blot transfer applications. Specifications Form: Liquid Concentration: 20 X Application: Nucleic Acid Gel Electrophoresis, Blotting Shipping Condition: Room Temperature Composition 3.0 M NaCl 0.2 M NaH2PO4 0.02 M EDTA pH 7.4 Storage: Room temperature MSDS

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TRIS-buffered saline (20x TBS) is used as a washing buffer for Western blot and other alkaline phosphatase and peroxidase conjugates assay, such as ELISA and dot blot assay. It acts as a pH stabilizer, which enable washing without disruption of antibody-antigen binding interactions. Each 20x TBS solution is ready to use upon dilution to the desired concentration.  Product Specification Appearance Clear, Colorless Form Liquid pH 7.6 ± 0.2 Composition (1x) 25 mM Tris, 137 mM NaCl, 2.7 mM KCl Comment All components are dissolved in ultrapure (Type I) water, followed by pH adjustment and filtered through 0.45 micron filter.  MSDS

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TRIS-buffered saline (20x TBS) is used as a washing buffer for Western blot and other alkaline phosphatase and peroxidase conjugates assay, such as ELISA and dot blot assay. It acts as a pH stabilizer, which enable washing without disruption of antibody-antigen binding interactions. Each 20x TBS solution is ready to use upon dilution to the desired concentration.  Product Specification Appearance Clear, Colorless Form Liquid pH 7.6 ± 0.2 Composition (1x) 25 mM Tris, 137 mM NaCl, 2.7 mM KCl Comment All components are dissolved in ultrapure (Type I) water, followed by pH adjustment and filtered through 0.45 micron filter.  MSDS

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TRIS-buffered saline (20x TBS) is used as a washing buffer for Western blot and other alkaline phosphatase and peroxidase conjugates assay, such as ELISA and dot blot assay. It acts as a pH stabilizer, which enable washing without disruption of antibody-antigen binding interactions. Each 20x TBS solution is ready to use upon dilution to the desired concentration.  Product Specification Appearance Clear, Colorless Form Liquid pH 7.6 ± 0.2 Composition (1x) 25 mM Tris, 137 mM NaCl, 2.7 mM KCl Comment All components are dissolved in ultrapure (Type I) water, followed by pH adjustment and filtered through 0.45 micron filter.  MSDS

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20x Tris-Acetate SDS Running Buffer is formulated for separation of proteins in their denatured state on Tris-Acetate gels. Tris-Acetate gels provide excellent separation of large molecular weight proteins when used with Tris-Acetate SDS Running Buffer, and LDS Sample Buffer. Tris-Acetate gels can also be run with Tris-Glycine Native Running Buffer and sample buffer to resolve native proteins more effectively than with the Tris-Glycine gel system. Composition 1x: Tris 50 mM, Tricine 50 mM, SDS 0.1%, pH 8.24 Storage 4°C for 1 year MSDS

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20x Tris-Acetate SDS Running Buffer is formulated for separation of proteins in their denatured state on Tris-Acetate gels. Tris-Acetate gels provide excellent separation of large molecular weight proteins when used with Tris-Acetate SDS Running Buffer, and LDS Sample Buffer. Tris-Acetate gels can also be run with Tris-Glycine Native Running Buffer and sample buffer to resolve native proteins more effectively than with the Tris-Glycine gel system. Composition 1x: Tris 50 mM, Tricine 50 mM, SDS 0.1%, pH 8.24 Storage 4°C for 1 year MSDS

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20x Tris-Acetate SDS Running Buffer is formulated for separation of proteins in their denatured state on Tris-Acetate gels. Tris-Acetate gels provide excellent separation of large molecular weight proteins when used with Tris-Acetate SDS Running Buffer, and LDS Sample Buffer. Tris-Acetate gels can also be run with Tris-Glycine Native Running Buffer and sample buffer to resolve native proteins more effectively than with the Tris-Glycine gel system. Composition 1x: Tris 50 mM, Tricine 50 mM, SDS 0.1%, pH 8.24 Storage 4°C for 1 year MSDS

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Current DNA electrophoresis buffers are mostly restricted to Tris-acetic acid-disodium EDTA (TAE) and Trisboric acid-disodium EDTA (TBE). The main problem of these buffers is that it generates excessive heat, limiting the voltage and speed of electrophoresis. Fast DNA Electrophoresis buffer overcomes this problem. The DNA gels can be run at high voltage (350V for 10cm gels in length) and are ready to be read in 15 minutes. The DNA extracted from the gel can be used for cloning. Usage Dilute in ddH2O to 1x Use diluted buffer to make gel and run the gel Voltage: 10-35V/cm, length of the gel Figure: Gel comprised 1.2% agarose. The gel was run fast (350 V) for 16 min in 1xFEB or 1xTBE. The run used a horizontal gel box,  700 mL total volume of buffers in the reservoirs and gels of 10 cm length. M, DNA marker (1 Kb Plus). PCR, 280-bp PCR fragment.

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Current DNA electrophoresis buffers are mostly restricted to Tris-acetic acid-disodium EDTA (TAE) and Tris-boric acid-disodium EDTA (TBE). The main problem of these buffers is that it generates excessive heat, limiting the voltage and speed of electrophoresis. Fast DNA Electrophoresis buffer overcomes this problem. The DNA gels can be run at high voltage (350V for 10cm gels in length) and are ready to be read in 15 minutes. The DNA extracted from the gel can be used for cloning. Usage Dilute in ddH2O to 1x Use diluted buffer to make gel and run the gel Voltage: 10-35V/cm, length of the gel Figure: Gel comprised 1.2% agarose. The gel was run fast (350 V) for 16 min in 1xFEB or 1xTBE. The run used a horizontal gel box,  700 mL total volume of buffers in the reservoirs and gels of 10 cm length. M, DNA marker (1 Kb Plus). PCR, 280-bp PCR fragment.

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Current DNA electrophoresis buffers are mostly restricte to Tris-acetic acid-disodium EDTA (TAE) and Trisboric acid-disodium EDTA (TBE). The main problem of these buffers is that it generates excessive heat, limiting the voltage and speed of electrophoresis. Fast DNA Electrophoresis buffer overcomes this problem. The DNA gels can be run at high voltage (350V for 10cm gels in length) and are ready to be read in 15 minutes. The DNA extracted from the gel can be used for cloning. Usage Dilute in ddH2O to 1x Use diluted buffer to make gel and run the gel Voltage: 10-35V/cm, length of the gel Figure: Gel comprised 1.2% agarose. The gel was run fast (350 V) for 16 min in 1xFEB or 1xTBE. The run used a horizontal gel box,  700 mL total volume of buffers in the reservoirs and gels of 10 cm length. M, DNA marker (1 Kb Plus). PCR, 280-bp PCR fragment.

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TRIS-buffered saline with Tween-20 (20x TBST) is used as a washing buffer for Western blot and other immunoassays, such as ELISA and dot blot assay. It acts as a pH stabilizer, which enable washing without disruption of antibody-antigen binding interactions. Tween 20 reduces nonspecific binding and protein: protein interactions. Each 10X TBST solution is ready to use upon dilution to the desired concentration.  Product Specification Appearance Clear, Colorless Form Liquid pH 7.6 ± 0.2 Composition (1x) 25mM Tris, 137mM NaCl, 2.7mM KCl, 0.05% Tween-20 Comment All components except Tween-20 are dissolved in ultrapure (Type I) water, followed by pH adjustment and filtered through 0.45 micron filter. Tween-20 is added and mixed before shipment. MSDS

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