Formulated for running proteins on Bis-Tris gels, including Invitrogen's NuPAGE® and Bolt Bis-Tris gel. MOPS/SDS Running Buffer is preferred for separating medium- to large-sized proteins. Both MES SDS Running Buffer and MOPS SDS Running Buffer can be used with Bis-Tris gels. MES has a lower pKa than MOPS, making the MES SDS Running Buffer faster than the MOPS SDS running buffers. The difference in ion migration affects stacking and results in a difference in protein separation range between these buffers. Use of MOPS buffer allows proteins to run slower than when using MES buffer. MOPS buffer is preferred for separating medium- to large-sized proteins. MES buffer is preferred for separating small- to medium-sized proteins.Composition: Tris 50 mM, MOPS 50 mM, SDS 0.1%, EDTA 1 mM, pH 7.7. (Exact same composition as NuPage and Bolt 20xMOPS Running Buffers) Compatibility: Bis-Tris precast gels, including Bolt gels and NuPAGE gel Storage: 4°C recommended. Shelf-life: 6 months, when stored properly. MSDS

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Formulated for running proteins on Bis-Tris gels, including Invitrogen's NuPAGE® and Bolt Bis-Tris gel. MOPS/SDS Running Buffer is preferred for separating medium- to large-sized proteins. Both MES SDS Running Buffer and MOPS SDS Running Buffer can be used with Bis-Tris gels. MES has a lower pKa than MOPS, making the MES SDS Running Buffer faster than the MOPS SDS running buffers. The difference in ion migration affects stacking and results in a difference in protein separation range between these buffers. Use of MOPS buffer allows proteins to run slower than when using MES buffer. MOPS buffer is preferred for separating medium- to large-sized proteins. MES buffer is preferred for separating small- to medium-sized proteins.Composition: Tris 50 mM, MOPS 50 mM, SDS 0.1%, EDTA 1 mM, pH 7.7. (Exact same composition as NuPage and Bolt 20xMOPS Running Buffers) Compatibility: Bis-Tris precast gels, including Bolt gels and NuPAGE gel Storage: 4°C recommended. Shelf-life: 6 months, when stored properly. MSDS

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Saline-Sodium Citrate Buffer (SSC buffer) is suitable for preparing nucleic acids for hybridization, hybridization buffer for northern and southern blots, washing buffer for northern and southern blots. SSC buffer is also used as a hybridization buffer in immunoblotting and DNA Microarray protocols. Appearance: Clear liquid pH: 7.0 ± 0.15 Method: Prepared in ultrapure (type I) water and filtered through 0.45 micron filter.  Composition: Sodium chloride:  3.0 M Sodium citrate dehydrate: 300 mM Storage: Room temperature Shelf life: 2 years MSDS

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Saline-Sodium Citrate Buffer (SSC buffer) is suitable for preparing nucleic acids for hybridization, hybridization buffer for northern and southern blots, washing buffer for northern and southern blots. SSC buffer is also used as a hybridization buffer in immunoblotting and DNA Microarray protocols. Appearance: Clear liquid pH: 7.0 ± 0.15 Method: Prepared in ultrapure (type I) water and filtered through 0.45 micron filter.  Composition: Sodium chloride:  3.0 M Sodium citrate dehydrate: 300 mM Storage: Room temperature Shelf life: 2 years MSDS

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20X SSPE is a prepared buffer concentrate for use in nucleic acid hybridizations and blot transfer applications. Specifications Form: Liquid Concentration: 20 X Application: Nucleic Acid Gel Electrophoresis, Blotting Shipping Condition: Room Temperature Composition 3.0 M NaCl 0.2 M NaH2PO4 0.02 M EDTA pH 7.4. Storage: Room Temperature MSDS

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20X SSPE is a prepared buffer concentrate for use in nucleic acid hybridizations and blot transfer applications. Specifications Form: Liquid Concentration: 20 X Application: Nucleic Acid Gel Electrophoresis, Blotting Shipping Condition: Room Temperature Composition 3.0 M NaCl 0.2 M NaH2PO4 0.02 M EDTA pH 7.4 Storage: Room temperature MSDS

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TRIS-buffered saline (20x TBS) is used as a washing buffer for Western blot and other alkaline phosphatase and peroxidase conjugates assay, such as ELISA and dot blot assay. It acts as a pH stabilizer, which enable washing without disruption of antibody-antigen binding interactions. Each 20x TBS solution is ready to use upon dilution to the desired concentration.  Product Specification Appearance Clear, Colorless Form Liquid pH 7.6 ± 0.2 Composition (1x) 25 mM Tris, 137 mM NaCl, 2.7 mM KCl Comment All components are dissolved in ultrapure (Type I) water, followed by pH adjustment and filtered through 0.45 micron filter.  MSDS

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TRIS-buffered saline (20x TBS) is used as a washing buffer for Western blot and other alkaline phosphatase and peroxidase conjugates assay, such as ELISA and dot blot assay. It acts as a pH stabilizer, which enable washing without disruption of antibody-antigen binding interactions. Each 20x TBS solution is ready to use upon dilution to the desired concentration.  Product Specification Appearance Clear, Colorless Form Liquid pH 7.6 ± 0.2 Composition (1x) 25 mM Tris, 137 mM NaCl, 2.7 mM KCl Comment All components are dissolved in ultrapure (Type I) water, followed by pH adjustment and filtered through 0.45 micron filter.  MSDS

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TRIS-buffered saline (20x TBS) is used as a washing buffer for Western blot and other alkaline phosphatase and peroxidase conjugates assay, such as ELISA and dot blot assay. It acts as a pH stabilizer, which enable washing without disruption of antibody-antigen binding interactions. Each 20x TBS solution is ready to use upon dilution to the desired concentration.  Product Specification Appearance Clear, Colorless Form Liquid pH 7.6 ± 0.2 Composition (1x) 25 mM Tris, 137 mM NaCl, 2.7 mM KCl Comment All components are dissolved in ultrapure (Type I) water, followed by pH adjustment and filtered through 0.45 micron filter.  MSDS

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20x Tris-Acetate SDS Running Buffer is formulated for separation of proteins in their denatured state on Tris-Acetate gels. Tris-Acetate gels provide excellent separation of large molecular weight proteins when used with Tris-Acetate SDS Running Buffer, and LDS Sample Buffer. Tris-Acetate gels can also be run with Tris-Glycine Native Running Buffer and sample buffer to resolve native proteins more effectively than with the Tris-Glycine gel system. Composition 1x: Tris 50 mM, Tricine 50 mM, SDS 0.1%, pH 8.24 Storage 4°C for 1 year MSDS

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20x Tris-Acetate SDS Running Buffer is formulated for separation of proteins in their denatured state on Tris-Acetate gels. Tris-Acetate gels provide excellent separation of large molecular weight proteins when used with Tris-Acetate SDS Running Buffer, and LDS Sample Buffer. Tris-Acetate gels can also be run with Tris-Glycine Native Running Buffer and sample buffer to resolve native proteins more effectively than with the Tris-Glycine gel system. Composition 1x: Tris 50 mM, Tricine 50 mM, SDS 0.1%, pH 8.24 Storage 4°C for 1 year MSDS

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20x Tris-Acetate SDS Running Buffer is formulated for separation of proteins in their denatured state on Tris-Acetate gels. Tris-Acetate gels provide excellent separation of large molecular weight proteins when used with Tris-Acetate SDS Running Buffer, and LDS Sample Buffer. Tris-Acetate gels can also be run with Tris-Glycine Native Running Buffer and sample buffer to resolve native proteins more effectively than with the Tris-Glycine gel system. Composition 1x: Tris 50 mM, Tricine 50 mM, SDS 0.1%, pH 8.24 Storage 4°C for 1 year MSDS

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