Grade: ULTRA PURE CAS Number: 77-86-1 Synonym(s): tris base; trizma; trizma base; 2-Amino-2-(hydroxymethyl)-1,3-propanediol; Tris(hydroxymethyl)aminomethane; Tris(hydroxymethyl)methylamine; THAM Molecular Formula: C4H11NO3 Molecular Weight: 121.14 g/mol Storage Condition: Room temperature Specification Result Appearance White crystalline Purity(By Titration 0.1 N HCL / Dried basis) 99.9% Melting Point (°C) 168.6-171.7 Moisture(By Karl Fischer) 0.12% pH (5.0% aqueous ) 10.7 Solubility (40% aqueous) Clear, colorless solution Ultraviolet Absorbance (40% aqueous) A (280 nm): 0.026A (260 nm): 0.002 Insoluble Matter in water ≤0.005%, Pass Ignition Residue 0.018% Fe ≤1ppm, Pass Heavy Metal ≤2ppm, Pass Chloride ≤1ppm, Pass Safety Data Sheet MSDS

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Grade: ULTRA PURE CAS Number: 77-86-1 Synonym(s): tris base; trizma; trizma base; 2-Amino-2-(hydroxymethyl)-1,3-propanediol; Tris(hydroxymethyl)aminomethane; Tris(hydroxymethyl)methylamine; THAM Molecular Formula: C4H11NO3 Molecular Weight: 121.14 g/mol Storage Condition: Room temperature Specification Result Appearance White crystalline Purity(By Titration 0.1 N HCL / Dried basis) 99.9% Melting Point (°C) 168.6-171.7 Moisture(By Karl Fischer) 0.12% pH (5.0% aqueous ) 10.7 Solubility (40% aqueous) Clear, colorless solution Ultraviolet Absorbance (40% aqueous) A (280 nm): 0.026A (260 nm): 0.002 Insoluble Matter in water ≤0.005%, Pass Ignition Residue 0.018% Fe ≤1ppm, Pass Heavy Metal ≤2ppm, Pass Chloride ≤1ppm, Pass Safety Data Sheet MSDS

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Q-PAGE™ TGN (Tris-Glycine Novel) Precast Gels are ready-to-use acrylamide gels for SDS-PAGE running in Tris-Glycine buffer system. With unique formula, Q-PAGE™ TGN Precast Gels perform enhanced speed, better separation, and longer shelf life as compared with conventional Laemmli Tris-HCl gels. The protein migration patterns in Q-PAGE™ TGN series, however, are similar with typical Laemmli Tris-HCl gels, and thus Q-PAGE™ TGN Precast Gels are compatible to traditional SDS-PAGE and subsequent analyses.  Suitable for Bio-Rad® and other systems. 12 wells/gel; 35ul loading capacity Key Features User-friendly gel cassette: Numbered and framed wells for sample loading Labeled warning sign and green tape as reminder Enhanced gel performance:  Enhanced gel electrophoresis speed  Better band separation  Stable for shipping at ambient temperature Easy compatibility:  Available as homogeneous and adjusted gradient gels for a wide range of protein separation. Compatible with most popular protein electrophoresis systems  Storage and stability Store Q-PAGE™ Precast Gels at 4°C for periods up to 12 months.

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Description  Q-PAGE TGN (Tris-Glycine Novel) Precast Gels are ready-to-use acrylamide gels for SDS-PAGE running in Tris-Glycine buffer system. With unique formula, Q-PAGE TGN Precast Gels perform enhanced speed, better separation, and longer shelf life as compared with conventional Laemmli Tris-HCl gels. The protein migration patterns in Q-PAGEâ„¢ TGN series, however, are similar with typical Laemmli Tris-HCl gels, and thus Q-PAGE  TGN Precast Gels are compatible to traditional SDS-PAGE and subsequent analyses.  Suitable for Invitrogen® XCell SureLock® Mini-Cell, Invitrogen®, Novex® Mini Gel Tank, Hoefer SE260, and other systems.  Key Features User-friendly gel cassette:  Numbered and framed wells for sample loading Labeled warning sign and green tape as reminder Enhanced gel performance:   Enhanced gel electrophoresis speed  Better band separation  Stable for shipping at ambient temperature Easy compatibility:   Available as homogeneous and adjusted gradient gels for a wide range of protein separation. Compatible with most popular protein electrophoresis systems  Storage and stability   Store Q-PAGE Precast Gels at 4°C for periods up to 12 months. Instruction  

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Description  Q-PAGE TGN (Tris-Glycine Novel) Precast Gels are ready-to-use acrylamide gels for SDS-PAGE running in Tris-Glycine buffer system. With unique formula, Q-PAGE TGN Precast Gels perform enhanced speed, better separation, and longer shelf life as compared with conventional Laemmli Tris-HCl gels. The protein migration patterns in Q-PAGEâ„¢ TGN series, however, are similar with typical Laemmli Tris-HCl gels, and thus Q-PAGE  TGN Precast Gels are compatible to traditional SDS-PAGE and subsequent analyses.  Suitable for Invitrogen® XCell SureLock® Mini-Cell, Invitrogen®, Novex® Mini Gel Tank, Hoefer SE260, and other systems.  Key Features User-friendly gel cassette:  Numbered and framed wells for sample loading Labeled warning sign and green tape as reminder Enhanced gel performance:   Enhanced gel electrophoresis speed  Better band separation  Stable for shipping at ambient temperature Easy compatibility:   Available as homogeneous and adjusted gradient gels for a wide range of protein separation. Compatible with most popular protein electrophoresis systems  Storage and stability   Store Q-PAGE Precast Gels at 4°C for periods up to 12 months. Instruction  

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Q-PAGE™ TGN (Tris-Glycine Novel) Precast Gels are ready-to-use acrylamide gels for SDS-PAGE running in Tris-Glycine buffer system. With unique formula, Q-PAGE™ TGN Precast Gels perform enhanced speed, better separation, and longer shelf life as compared with conventional Laemmli Tris-HCl gels. The protein migration patterns in Q-PAGE™ TGN series, however, are similar with typical Laemmli Tris-HCl gels, and thus Q-PAGE™ TGN Precast Gels are compatible to traditional SDS-PAGE and subsequent analyses.  Suitable for Bio-Rad® and other systems.  15 wells/gel; 22ul loading capacity Key Features User-friendly gel cassette: Numbered and framed wells for sample loading Labeled warning sign and green tape as reminder Enhanced gel performance:  Enhanced gel electrophoresis speed  Better band separation  Stable for shipping at ambient temperature Easy compatibility:  Available as homogeneous and adjusted gradient gels for a wide range of protein separation. Compatible with most popular protein electrophoresis systems  Storage and stability Store Q-PAGE™ Precast Gels at 4°C for periods up to 12 months.

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Grade: ULTRA PURE CAS Number: 1185-53-1 Synonym(s): Tris HCl, 2-Amino-2-hydroxymethyl-1 3-propanediol hydrochloride,Tris(hydroxymethyl) aminomethane hydrochloride Molecular Weight: 157.64 Molecular Formula: C4H11NO3.HCl Storage Condition: Room temperature Specification Result Appearance White crystalline Infrafed Pass Purity (By Titration 0.1N NaOH/Dried basis) 99.7% Melt point 150.2-152.8°C Water Content (By Karl Fischer) 0.35% Loss on drying 0.12% pH (1.0% aqueous ) 4.8 Ultraviolet Absorbance (1.0M aqueous) A(260 nm): 0.022A(280 nm): 0.020 Solubility (1.0M aqueous) Clear, colorless solution Heavy metal ≤5ppm Fe ≤5ppm RNase (P/F) None DNase (P/F) None Safety Data Sheet MSDS

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Grade: ULTRA PURE CAS Number: 1185-53-1 Synonym(s): Tris HCl, 2-Amino-2-hydroxymethyl-1 3-propanediol hydrochloride,Tris(hydroxymethyl) aminomethane hydrochloride Molecular Weight: 157.64 Molecular Formula: C4H11NO3.HCl Storage Condition: Room temperature Specification Result Appearance White crystalline Infrafed Pass Purity (By Titration 0.1N NaOH/Dried basis) 99.7% Melt point 150.2-152.8°C Water Content (By Karl Fischer) 0.35% Loss on drying 0.12% pH (1.0% aqueous ) 4.8 Ultraviolet Absorbance (1.0M aqueous) A(260 nm): 0.022A(280 nm): 0.020 Solubility (1.0M aqueous) Clear, colorless solution Heavy metal ≤5ppm Fe ≤5ppm RNase (P/F) None DNase (P/F) None Safety Data Sheet MSDS

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Two Step RT-PCR Kit consists of two main components: PowerScript™ Plus cDNA Synthesis MasterMix for reverse transcription 2XTaqPlus PCR MasterMix for subsequent PCR Composition: PowerScript™ Plus Reverse Transcriptase (200U/µl, 30µl) 2xReaction mix contains Ribonuclease Inhibitor, dNTPs, Oligo(dT)s and Random Primers, 350µl Nuclease-free H2O, 1ml  2XTaqPlus PCR MasterMix, 1ml Applications Gene-expression analysis Transcription analysis Gene cloning Multiplex RT-PCR High throughput applications Virus detection and quantification

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