Current DNA electrophoresis buffers are mostly restricte to Tris-acetic acid-disodium EDTA (TAE) and Trisboric acid-disodium EDTA (TBE). The main problem of these buffers is that it generates excessive heat, limiting the voltage and speed of electrophoresis. Fast DNA Electrophoresis buffer overcomes this problem. The DNA gels can be run at high voltage (350V for 10cm gels in length) and are ready to be read in 15 minutes. The DNA extracted from the gel can be used for cloning.
Usage
- Dilute in ddH2O to 1x
- Use diluted buffer to make gel and run the gel
- Voltage: 10-35V/cm, length of the gel
Figure: Gel comprised 1.2% agarose. The gel was run fast (350 V) for 16 min in 1xFEB or 1xTBE. The run used a horizontal gel box, 700 mL total volume of buffers in the reservoirs and gels of 10 cm length. M, DNA marker (1 Kb Plus). PCR, 280-bp PCR fragment.
