10X TBE Buffer (Tris-borate-EDTA) is the most commonly used buffer for DNA and RNA polyacrylamide gel electrophoresis. It is also routinely used for DNA automated sequencing gel. TBE can also be used for agarose gels, but is not recommended for preparative gels for recovery of nucleic acids. Since borate in TBE buffer is a strong inhibitor for many enzymes, TAE buffer is recommended when looking at enzymatic applications for the DNA sample.
Applications
- Electrophoresis of nucleic acids in agarose and polyacrylamide gels
- Used both as a running buffer and as a gel preparation buffer
- Filtered through a 0.45 μm membrane
- Recommended for electrophoresis of RNA and DNA fragments smaller than 1500 bp
Composition
10x: Tris 890 mM, Boric acid 890 mM, EDTA 20 mM, pH 8.3
MSDS
