| Name | 1x Composition | Applications |
| 10x PBS | Phosphate buffer 10 mM, KCl 2.7 mM, NaCl 137 mM, pH 7.4 | Most widely used washing buffer for Western blot, ELISA, Immuno-Histochemistry staining et al. |
| 10x PBST | Phosphate buffer 10 mM, KCl 2.7 mM, NaCl 137 mM, Tween-20 0.05%, pH 7.4 | Western blot, ELISA, Immuno-Histochemical staining et. al. |
| 10x TBS | TBS01: Tris 50 mM, NaCl 150 mM, pH 7.4 | Western blot, ELISA, Immuno-Histochemistry |
| 20x TBS | TBS03, TBS04: Tris 25 mM, NaCl 137 mM, KCl 2.7 mM, pH 7.6 | Western blot, ELISA, Immuno-Histochemistry |
| 10x TBST | TBST01: Tris 50 mM, NaCl 150 mM, Tween-20 0.05%, pH 7.4 | Western blot, ELISA, Immuno-Histochemistry |
| 20x TBST | TBST03, TBST04: Tris 25mM, NaCl 137mM, KCl 2.7mM, Tween 0.05%, pH 7.6 | Western blot, ELISA, Immuno-Histochemistry |
Washing Buffers
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20x TBS Buffer (10 L)
TRIS-buffered saline (20x TBS) is used as a washing buffer for Western blot and other alkaline phosphatase and peroxidase conjugates assay, such as ELISA and dot blot assay. It acts as a pH stabilizer, which enable washing without disruption of antibody-antigen binding interactions. Each 20x TBS solution is ready to use upon dilution to the desired concentration. Product Specification Appearance Clear, Colorless Form Liquid pH 7.6 ± 0.2 Composition (1x) 25 mM Tris, 137 mM NaCl, 2.7 mM KCl Comment All components are dissolved in ultrapure (Type I) water, followed by pH adjustment and filtered through 0.45 micron filter. MSDS
$240.00
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20xTBST Buffer (10 L)
TRIS-buffered saline with Tween-20 (20x TBST) is used as a washing buffer for Western blot and other immunoassays, such as ELISA and dot blot assay. It acts as a pH stabilizer, which enable washing without disruption of antibody-antigen binding interactions. Tween 20 reduces nonspecific binding and protein: protein interactions. Each 10X TBST solution is ready to use upon dilution to the desired concentration. Product Specification Appearance Clear, Colorless Form Liquid pH 7.6 ± 0.2 Composition (1x) 25mM Tris, 137mM NaCl, 2.7mM KCl, 0.05% Tween-20 Comment All components except Tween-20 are dissolved in ultrapure (Type I) water, followed by pH adjustment and filtered through 0.45 micron filter. Tween-20 is added and mixed before shipment. MSDS
$250.00
