The EasyPrep™ Plasmid Miniprep Kit provides a simple, fast and cost effective method to purify plasmid DNAs (10-30 µg) from small volumes of bacterial culture (1-5 ml), which is typical in bacterial colony screening during plasmid construction. The kit applies DNA-binding specific matrix, which allows the high efficient binding of DNA, while proteins and other cellular components are easily removed during washing. Plasmid DNAs are easily eluted with Tris buffer or pure water. Plasmid DNAs purified from EasyPrep™ plasmid miniprep kit can be used directly for downstream applications such as transfection, restriction mapping, library screening, sequencing, as well as gene therapy and genetic vaccinations. Kit's Contents SKU PD01-01 PD01-02 Preps 50 250 DNA Columns 50 250 Buffer A1 20 ml 80 ml Buffer A2 20 ml 80 ml Buffer N1 25 ml 110 ml DNA Wash Buffer* 12 ml 50 ml Elution Buffer 10 ml 20 ml Column Buffer 10 ml 30 ml RNase A 70 µl 280 µl Manual 1 1 * Add ethanol to washing buffer before use

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The EasyPrep™ Plasmid Miniprep-II Kit provides a simple, fast and cost effective method to purify plasmid DNAs from bacterial culture, especially useful for low-copy-number plasmid miniprep or applications  that amount of DNA needed is about 30-50 µg. The kit applies DNA-binding-specific matrix, which allows the high efficient binding of DNA, while proteins and other cellular components are easily removed during washing. Plasmid DNAs are easily eluted with Tris buffer or pure water. Plasmid DNAs purified from EasyPrep™ plasmid Miniprep-II kit can be used directly for downstream applications such as transfection, restriction mapping, library screening, sequencing, as well as gene therapy and genetic vaccinations. Kit's Contents SKU PD02-01 PD02-02 Miniprep-II DNA Columns 50 250 Buffer A1 30 ml 130 ml Buffer A2 30 ml 130 ml Buffer N1 45 ml 180 ml DNA Wash Buffer Concentrate* 20 ml 54 ml Elution Buffer 10 ml 30 ml Column Buffer  10 ml 30 ml RNase A 110 µl 440 µl 2ml Collection tubes 50 250 Manual 1 1 * Add ethanol to washing buffer before use  

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Key to the kit is our proprietary DNA binding systems that allow the high efficient reversible binding of DNA to the mini column while proteins and other impurities are removed by wash buffer. Nucleic acids are then eluted with sterile water or elution buffer. Plasmid isolated with traditional protocol normally contains high level of endotoxins (lipopolysaccharides or LPS). For transfection of endotoxin sensitive cell lines or microinjection, the endotoxins should be removed before the applications. The EasyPrep™ endofree system uses a specially formulated buffer that extracts the endotoxin from the plasmid DNA. Two rounds of extraction will reduce the endotoxin level to 0.1 EU (Endotoxin) per µg of plasmid DNA. The endofree plasmid miniprep kit provides an efficient endotoxin removal step into the traditional purification procedure to produce transfection grade plasmid DNA. This kit is designed for fast and efficient purification of plasmid DNA from 3 to 10 mL of E. coli culture. The mini column II has a DNA binding capacity of 80 µg. The purified endofree DNA is ready for downstream applications such as transfection of endotoxin-sensitive cell lines, primary cultured cells or microinjection. Kit's Contents SKU PD02-11 PD02-12 Preps 50 250 DNA Miniprep-II Columns 50 250 Buffer A1 25 mL 125 mL Buffer A2 25 mL 125 mL Buffer N3 5 mL 25 mL Buffer KB 30 mL 135 mL Buffer RET 50 mL 250 mL DNA Wash Buffer* 20 mL 3 x 24 mL EndoFree Elution Buffer 10 mL 30 mL RNase A 175 μL 625 μL User Manual 1 1 * Add ethanol to washing buffer before use  

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The EasyPrep 96-well Plasmid Miniprep kit provides an easy and fast method for isolating high quality plasmid DNA in a high through put format. The key to this system is that the DNA columns avidly but reversibly binds DNA under optimized buffer conditions, while proteins and other unwanted cellular components are removed by wash buffer. High quality plasmid DNAs are collected for downstream applications. By using the 96-well kit, up to 96 samples can be simultaneously processed in less than 90 min. The lysate clearance plate obviates the time-consuming centrifugation step and increases the DNA recovery up to 20%. Kit's Contents SKU PD96-01 PD96-11 PD96-02 PD96-12 Preps 4 20 96- Well 2.2 mL Plate 4 20 96- Well 1.6 mL Plate 2 2 96-Well DNA Plate 4 20 96-well lysate clearance plate 4 (PD96-01) 20 (PD96-02) 96-Well Collection Plate 4 20 Ventilating Film 4 20 Sealing Film 16 80 Buffer A1 110 mL 550 mL Buffer A2 110 mL 550 mL Buffer N1 160 mL 2x400 mL DNA Wash Buffer 2x125 mL 7x125mL Buffer KB 240 mL 1200 mL Elution Buffer 100 mL 500 mL RNase A 600 μL 3 mL User Manual 1 1

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Used for plasmid or genomic DNA miniprep purification. Also can be used for gel extraction and PCR cleaning Column binding capacity: 30 ug. Column Buffer included. Column Buffer is used for column equilibration, providing more consistent DNA yield when the columns stored for a while.  Compatible with all other commercial kit buffers, including Invitrogen, Qiagen, Promega. Collection tubes included

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The columns provide as much as 80 ug DNA binding capacity. If more DNA needed for testing, such as EndoFree DNA purification or use plasmid for transfection, the column is a good option.  Compatible with all other commercial kit buffers, including Invitrogen, Qiagen, Promega.

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