EasyPrep™ Plasmid Midiprep-II Kit provides a simple, fast and cost effective method to purify plasmid DNAs from bacterial culture, when amount of the plasmid DNA needed is about 400-500 µg. The kit is especially convenient to use when multiple plasmids need to be purified at the same time, as the use of traditional commercial kits is time-consuming. EasyPrepTM Plasmid Midiprep-II Kit applies DNA-binding-specific matrix, which allows the high efficient binding of DNA, while proteins and other cellular components are easily removed during washing. Plasmid DNAs are easily eluted with Tris buffer or pure water. Plasmid DNAs purified from EasyPrep™ Plasmid Midiprep-II Kit can be used directly for downstream applications such as transfection, restriction mapping, library screening, sequencing, as well as gene therapy and genetic vaccinations. Kit's Contents SKU PD04-01PD04-11 PD04-02PD04-12 Midiprep DNA Columns 10 25 EasyFilter 10 (PD04-11) 25 (PD04-12) Buffer A1 60 ml 150 ml Buffer A2 60 ml 150 ml Buffer A3 75 ml 180 ml Elution Buffer 10 ml 20 ml Column Buffer 35 ml 80 ml RNase A 210 µl 520 µl 50ml conical tubes 10 25 Manual 1 1  

Read more less

EasyPrep™ Plasmid Midiprep-II Kit provides a simple, fast and cost effective method to purify plasmid DNAs from bacterial culture, when amount of the plasmid DNA needed is about 400-500 µg. The kit is especially convenient to use when multiple plasmids need to be purified at the same time, as the use of traditional commercial kits is time-consuming. EasyPrepTM Plasmid Midiprep-II Kit applies DNA-binding-specific matrix, which allows the high efficient binding of DNA, while proteins and other cellular components are easily removed during washing. Plasmid DNAs are easily eluted with Tris buffer or pure water. Plasmid DNAs purified from EasyPrep™ Plasmid Midiprep-II Kit can be used directly for downstream applications such as transfection, restriction mapping, library screening, sequencing, as well as gene therapy and genetic vaccinations. Kit's Contents SKU PD04-01PD04-11 PD04-02PD04-12 Midiprep DNA Columns 10 25 EasyFilter 10 (PD04-11) 25 (PD04-12) Buffer A1 60 ml 150 ml Buffer A2 60 ml 150 ml Buffer A3 75 ml 180 ml Elution Buffer 10 ml 20 ml Column Buffer 35 ml 80 ml RNase A 210 µl 520 µl 50ml conical tubes 10 25 Manual 1 1  

Read more less

EasyPrep™ EndoFree Plasmid Midiprep-II Kit provides a simple, fast and cost effective method to purify plasmid DNAs from bacterial culture, when amount of the plasmid DNA needed is about 500 µg. The kit is especially convenient to use when multiple plasmids need to be purified at the same time, as the use of traditional commercial kits is time-consuming. Key to the kit is our proprietary DNA binding systems that allow the high efficient DNA binding  while proteins and other impurities are removed by wash buffer. Nucleic acids are easily eluted with Elution Buffer. Our proprietary endotoxin removal buffer, Buffer RET, is designed to remove endotoxin by a single washing step without tedious phase partitioning steps. The purified plasmid DNA, with endotoxin level less than 0.1 EU per µg of DNA, is ready for transfection of endotoxin sensitive cell lines and microinjections. Kit's Content SKU PD04-21PD04-31 PD04-22PD04-32 Midiprep-II DNA Columns 10 25 ezFilter 10(PD04-31) 25(PD04-32) Buffer A1 60 ml 150 ml Buffer B1 60 ml 150 ml Buffer N3 30 ml 75 ml Buffer RET 60 ml 150 ml DNA Wash Buffer (5x)* 54 ml 54 ml EndoFree Elution Buffer (H2O) 15 ml 30 ml RNase A 220 µl 520 µl Manual 1 1 * Add ethanol to washing buffer before use

Read more less

EasyPrep™ EndoFree Plasmid Midiprep-II Kit provides a simple, fast and cost effective method to purify plasmid DNAs from bacterial culture, when amount of the plasmid DNA needed is about 500 µg. The kit is especially convenient to use when multiple plasmids need to be purified at the same time, as the use of traditional commercial kits is time-consuming. Key to the kit is our proprietary DNA binding systems that allow the high efficient DNA binding  while proteins and other impurities are removed by wash buffer. Nucleic acids are easily eluted with Elution Buffer. Our proprietary endotoxin removal buffer, Buffer RET, is designed to remove endotoxin by a single washing step without tedious phase partitioning steps. The purified plasmid DNA, with endotoxin level less than 0.1 EU per µg of DNA, is ready for transfection of endotoxin sensitive cell lines and microinjections. Kit's Content SKU PD04-21PD04-31 PD04-22PD04-32 Midiprep-II DNA Columns 10 25 ezFilter 10(PD04-31) 25(PD04-32) Buffer A1 60 ml 150 ml Buffer B1 60 ml 150 ml Buffer N3 30 ml 75 ml Buffer RET 60 ml 150 ml DNA Wash Buffer (5x)* 54 ml 54 ml EndoFree Elution Buffer (H2O) 15 ml 30 ml RNase A 220 µl 520 µl Manual 1 1 * Add ethanol to washing buffer before use

Read more less

EasyPrep™ EndoFree Plasmid Midiprep-II Kit provides a simple, fast and cost effective method to purify plasmid DNAs from bacterial culture, when amount of the plasmid DNA needed is about 500 µg. The kit is especially convenient to use when multiple plasmids need to be purified at the same time, as the use of traditional commercial kits is time-consuming. Key to the kit is our proprietary DNA binding systems that allow the high efficient DNA binding  while proteins and other impurities are removed by wash buffer. Nucleic acids are easily eluted with Elution Buffer. Our proprietary endotoxin removal buffer, Buffer RET, is designed to remove endotoxin by a single washing step without tedious phase partitioning steps. The purified plasmid DNA, with endotoxin level less than 0.1 EU per µg of DNA, is ready for transfection of endotoxin sensitive cell lines and microinjections. Kit's Content SKU PD04-21PD04-31 PD04-22PD04-32 Midiprep-II DNA Columns 10 25 ezFilter 10(PD04-31) 25(PD04-32) Buffer A1 60 ml 150 ml Buffer B1 60 ml 150 ml Buffer N3 30 ml 75 ml Buffer RET 60 ml 150 ml DNA Wash Buffer (5x)* 54 ml 54 ml EndoFree Elution Buffer (H2O) 15 ml 30 ml RNase A 220 µl 520 µl Manual 1 1 * Add ethanol to washing buffer before use

Read more less

EasyPrep™ EndoFree Plasmid Midiprep-II Kit provides a simple, fast and cost effective method to purify plasmid DNAs from bacterial culture, when amount of the plasmid DNA needed is about 500 µg. The kit is especially convenient to use when multiple plasmids need to be purified at the same time, as the use of traditional commercial kits is time-consuming. Key to the kit is our proprietary DNA binding systems that allow the high efficient DNA binding  while proteins and other impurities are removed by wash buffer. Nucleic acids are easily eluted with Elution Buffer. Our proprietary endotoxin removal buffer, Buffer RET, is designed to remove endotoxin by a single washing step without tedious phase partitioning steps. The purified plasmid DNA, with endotoxin level less than 0.1 EU per µg of DNA, is ready for transfection of endotoxin sensitive cell lines and microinjections. Kit's Content SKU PD04-21PD04-31 PD04-22PD04-32 Midiprep-II DNA Columns 10 25 ezFilter 10(PD04-31) 25(PD04-32) Buffer A1 60 ml 150 ml Buffer B1 60 ml 150 ml Buffer N3 30 ml 75 ml Buffer RET 60 ml 150 ml DNA Wash Buffer (5x)* 54 ml 54 ml EndoFree Elution Buffer (H2O) 15 ml 30 ml RNase A 220 µl 520 µl Manual 1 1 * Add ethanol to washing buffer before use

Read more less